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公开(公告)号：US10167509B2
公开(公告)日：2019-01-01
申请号：US14848311
申请日：2015-09-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/68 , C07H21/00 , C12Q1/6883 , C12Q1/683 , C12Q1/6858
Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.
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公开(公告)号：US09921154B2
公开(公告)日：2018-03-20
申请号：US14099750
申请日：2013-12-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Yann Jouvenot , Serge Saxonov , Simant Dube , John Frederick Regan
CPC classification number: G01N21/64 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G06F19/12 , G06F19/18
Abstract: System, including methods and apparatus, for performing a multiplexed digital assay.
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公开(公告)号：US20160108464A1
公开(公告)日：2016-04-21
申请号：US14981134
申请日：2015-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Serge Saxonov , Simant Dube , Benjamin J. Hindson , Adam M. McCoy
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6851 , G06F17/10 , G06F17/11 , G06F17/12 , G16B5/00 , G16B20/00 , G16B25/00 , C12Q2537/143 , C12Q2537/165 , C12Q2563/159
Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.
Abstract translation: 用于通过组合使用信号对更多数量目标进行多路复用数字测定的系统，包括方法，装置和组合物。
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公开(公告)号：US20140171341A1
公开(公告)日：2014-06-19
申请号：US14099750
申请日：2013-12-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Yann Jouvenot , Serge Saxonov , Simant Dube , John Frederick Regan
IPC: G01N21/64
CPC classification number: G01N21/64 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G06F19/12 , G06F19/18
Abstract: System, including methods and apparatus, for performing a multiplexed digital assay.
Abstract translation: 用于执行多路复用数字测定的系统，包括方法和装置。
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公开(公告)号：US20250091048A1
公开(公告)日：2025-03-20
申请号：US18888785
申请日：2024-09-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Serge Saxonov , Phillip Belgrader , Kevin D. Ness , Michael Y. Lucero , Billy W. Colston, JR. , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
IPC: B01L3/00 , B01F23/41 , B01F25/00 , B01F25/23 , B01F33/3011 , B01F33/302 , B01F33/81 , B01L9/00 , C12Q1/686 , G01N35/08
Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
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Patent Agency Ranking
公开(公告)号：US12097495B2
公开(公告)日：2024-09-24
申请号：US17750195
申请日：2022-05-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. Hindson , Serge Saxonov , Phillip Belgrader , Kevin D. Ness , Michael Y. Lucero , Billy W. Colston, Jr. , Shawn Paul Hodges , Nicholas J. Heredia , Jeffrey Clark Mellen , Camille Bodley Troup , Paul Wyatt
IPC: B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC classification number: B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
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公开(公告)号：US20180147573A1
公开(公告)日：2018-05-31
申请号：US15707908
申请日：2017-09-18
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, Jr. , Erin R. Chia , Billy W. Colston, Jr. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
IPC: B01L3/00 , B01L9/00 , B01F13/00 , C12Q1/686 , B01F13/10 , B01F3/08 , B01F5/02 , B01F5/00 , G01N35/08
CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08
Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.
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公开(公告)号：US09885034B2
公开(公告)日：2018-02-06
申请号：US14493268
申请日：2014-09-22
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Serge Saxonov
CPC classification number: C12N15/1065 , C12N15/10 , C12N15/1075 , C12Q1/6876 , C12Q2525/191 , C12Q2563/179
Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
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公开(公告)号：US20150031034A1
公开(公告)日：2015-01-29
申请号：US14493264
申请日：2014-09-22
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin Hindson , Serge Saxonov , Philip Belgrader , Kevin Ness , Michael Lucero , Billy Colston
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , C12Q1/6827 , C12Q2537/157 , C12Q2563/161 , C12Q2533/107 , C12Q2521/319
Abstract: This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
Abstract translation: 本发明提供了用于检测靶多核苷酸拷贝数差异的组合物和方法。 在一些情况下，当起始样品是母体组织（例如血液，血浆）时，本文提供的方法和组合物可用于诊断胎儿遗传异常。 描述的方法和材料适用于允许检测多核苷酸拷贝数的小但统计学上显着的差异的技术。
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公开(公告)号：US20230372935A1
公开(公告)日：2023-11-23
申请号：US18362530
申请日：2023-07-31
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz , Erin R. Chia , Billy W. Colston , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC classification number: B01L3/50273 , B01F23/41 , B01F33/813 , B01F33/3011 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/23
Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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