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88 条记录 (耗时 0.051 秒)

    Capillary electrokinesis based cellular assays
    17.
    发明授权
    Capillary electrokinesis based cellular assays 有权
    基于毛细管电活动的细胞检测

    公开(公告)号：US06613211B1

    公开(公告)日：2003-09-02

    申请号：US09642607

    申请日：2000-08-17

    申请人： Randy M. McCormick ,  Gary J. Ciambrone ,  Ian Gibbons

    发明人： Randy M. McCormick ,  Gary J. Ciambrone ,  Ian Gibbons

    IPC分类号： C02F140

    CPC分类号： G01N33/5005 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/0418 ,  G01N27/44791

    摘要： Cell based assays are performed in a microfluidic device, where the cells are introduced into a reservoir and are contacted with one or more agents prior to or during their residence in the reservoir or in a capillary channel connected to the reservoir. The cells are moved by electrokinesis individually from the reservoir to a detector, where the status of the cells as a result of contacting said agents is determined. Conditions are provided for moving the cells electrophoretically or by electroosmotic force, where the cells may be viable or fixed, natural or genetically modified.

    摘要翻译： 在微流体装置中进行基于细胞的测定，其中将细胞引入储存器中并且在它们停留在储存器中或在与储存器连接的毛细通道中之前或期间与一种或多种试剂接触。 细胞通过电活动单独地从储存器移动到检测器，其中确定作为接触所述试剂的结果的细胞的状态。 提供用于电泳或通过电渗力移动细胞的条件，其中细胞可存活或固定，天然或遗传修饰。

    Dilution and mixing cartridge
    19.
    发明授权
    Dilution and mixing cartridge 失效
    稀释和混合盒

    公开(公告)号：US5104813A

    公开(公告)日：1992-04-14

    申请号：US337286

    申请日：1989-04-13

    申请人： Don Besemer ,  Michael Gorin ,  Ian Gibbons

    发明人： Don Besemer ,  Michael Gorin ,  Ian Gibbons

    IPC分类号： G01F13/00 ,  B01L3/00 ,  G01N1/00 ,  G01N1/38 ,  G01N33/04 ,  G01N33/48

    CPC分类号： G01N1/38 ,  B01L3/5027 ,  B01L3/502723 ,  G01N33/04 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： A dilution and mixing cartridge is provided that allows single (or multiple) dilutions of a sample with a diluent in a disposable cartridge in which a measurement, such as optical density, is made. Addition of sample to the device automatically measures the sample, and addition of diluent automatically causes a fixed ratio of sample and diluent to enter a receiving chamber, in which mixing and measurement can take place. A number of preferred embodiments are provided in which the diluent application site is located at a vertical height below the sample application site, flow directing chambers are provided to control flow of desired volumes of sample or diluted sample at the appropriate locations in the device and to direct excess sample and diluent to waste locations, or control mechanism are provided at flow directing chambers and other internal locations to verify correct operation of the device.

    Liquid single reagent for assays involving confining gels
    20.
    发明授权
    Liquid single reagent for assays involving confining gels 失效
    液体单一试剂用于测定凝胶

    公开(公告)号：US5068198A

    公开(公告)日：1991-11-26

    申请号：US298192

    申请日：1989-01-13

    申请人： Ian Gibbons ,  Edwin F. Ullman ,  Philip L. Felgner

    发明人： Ian Gibbons ,  Edwin F. Ullman ,  Philip L. Felgner

    IPC分类号： G01N33/543 ,  G01N33/549

    CPC分类号： G01N33/549 ,  G01N33/54313 ,  Y10S436/829

    摘要： Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured. Examples of the confining material are lipid bilayers, cells and gels.

    摘要翻译： 提供测定方法和组合物用于测定怀疑含有分析物的样品中的分析物。 组合物包含新颖的单液体试剂至少一种特异性结合对（sbp）构件及其互补构件，其中至少一个sbp构件可逆地限制在暂时使限制的sbp构件不能与其互补的sbp构件结合的材料中 。 sbp成员中的至少一个与能够产生与样品中分析物的量相关的可检测信号的信号产生系统的成员结合。 约束相反，添加信号产生系统的任何剩余成员，并且测量相对于分析物量产生的信号。 限制材料的实例是脂双层，细胞和凝胶。