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公开(公告)号：US11982620B2
公开(公告)日：2024-05-14
申请号：US17441258
申请日：2020-03-17
发明人： Shaohong Wang
CPC分类号： G01N21/645 , G01N21/33 , G01N2021/174 , G01N2021/6463
摘要： An optical detection system for a capillary electrophoresis instrument is disclosed. The system includes an ultraviolet (UV) source and an absorption measurement optical path. In an embodiment, the optical path comprises a first plurality of optical elements arranged to obtain a plurality of respective UV beamlets from a UV beam emitted by the UV source and to direct the respective UV beamlets transversely through respective capillaries of a plurality of capillaries and to an absorption detector positioned to detect respective signals for use in obtaining respective UV absorption measurements corresponding to the respective capillaries.
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公开(公告)号：US11959074B2
公开(公告)日：2024-04-16
申请号：US17525737
申请日：2021-11-12
IPC分类号： C12N15/10 , C12Q1/6825 , C12Q1/6874 , G01N35/10
CPC分类号： C12N15/1065 , C12Q1/6825 , C12Q1/6874 , G01N35/1081
摘要： A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.
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公开(公告)号：US20240102003A1
公开(公告)日：2024-03-28
申请号：US18345856
申请日：2023-06-30
发明人： Arvydas Lubys , Inga Cikotiene , Zana Kapustina , Arturas Berezniakovas , Justina Sulgaite , Simona Zeimyte , Mark Andersen , Michael Allen , Sihong Chen
IPC分类号： C12N15/10 , C12N15/115 , C12Q1/6806
CPC分类号： C12N15/1065 , C12N15/115 , C12Q1/6806 , C12N2310/3517
摘要： The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligo-nucleotides can be used in a variety of nucleic acid sequencing methods.
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公开(公告)号：US20240101980A1
公开(公告)日：2024-03-28
申请号：US18521466
申请日：2023-11-28
发明人： Daniel MAZUR , Sihong CHEN , Peter VANDER HORN , Eileen TOZER , Guobin LUO , Joshua SHIRLEY , Kevin HEINEMANN
CPC分类号： C12N9/1252 , C12Q1/686 , C12Y207/07007
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.
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25.发明公开公开(公告)号：US20240094110A1
公开(公告)日：2024-03-21
申请号：US18240441
申请日：2023-08-31
IPC分类号： G01N15/14
CPC分类号： G01N15/1436 , G01N2015/0065
摘要： Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.
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公开(公告)号：US20240067939A1
公开(公告)日：2024-02-29
申请号：US18221099
申请日：2023-07-12
发明人： Chieh-Yuan LI , David RUFF , Shiaw-Min CHEN , Jennifer O'NEIL , Rachel KASINSKAS , Jonathan ROTHBERG , Bin LI , Kai Qin LAO
IPC分类号： C12N9/12 , C12Q1/6844 , C12Q1/6853 , C12Q1/6855 , C12Q1/686
CPC分类号： C12N9/1252 , C12Q1/6846 , C12Q1/6853 , C12Q1/6855 , C12Q1/686 , C12Q1/6874
摘要： The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.
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公开(公告)号：US11890557B2
公开(公告)日：2024-02-06
申请号：US17740710
申请日：2022-05-10
发明人： Charles R. Rhodes
IPC分类号： B01D29/27 , C12M1/12 , C12M1/00 , C12M1/26 , B01D35/027
CPC分类号： B01D29/27 , C12M23/14 , C12M23/28 , C12M25/16 , C12M29/04 , C12M33/14 , C12M47/02 , B01D35/027
摘要： A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.
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公开(公告)号：US11879156B2
公开(公告)日：2024-01-23
申请号：US17818869
申请日：2022-08-10
发明人： Phil Waggoner , James A. Ball , Wolfgang Hinz , Michael L. Minto , Scott Parker , David M. Cox , Alexander Mastroianni , Jeremy Gray , Marc Glazer , Kimberly Gorrell
IPC分类号： G01N27/414 , C12Q1/6869
CPC分类号： C12Q1/6869 , G01N27/4145 , C12Q1/6869 , C12Q2527/119 , C12Q2527/125 , C12Q2565/607
摘要： A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
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公开(公告)号：US20240021272A1
公开(公告)日：2024-01-18
申请号：US18330737
申请日：2023-06-07
发明人： Fiona HYLAND , Eric TSUNG , Vasisht TADIGOTLA , Zheng ZHANG , Dumitru BRINZA , Onur SAKARYA , Xing XU
摘要： Systems and method for determining variants can receive mapped reads, and call variants. In embodiments, flow space information for the reads can be aligned to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be grouped and a score can be calculated for the variant. Based on the scores, a list of probable variants can be provided. In various embodiments, low frequency variants can be identified where multiple potential variants are present at a position.
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公开(公告)号：US11866740B2
公开(公告)日：2024-01-09
申请号：US17302621
申请日：2021-05-07
发明人： Daniel Mazur , Peter B. Vander Horn , Eileen Tozer , Sihong Chen , Guobin Luo , Joshua Shirley , Kevin Heinemann
CPC分类号： C12N9/1252 , C12Q1/686 , C12Y207/07007 , C12Q1/686 , C12Q2521/101
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.
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