公开(公告)号：US07517690B2

公开(公告)日：2009-04-14

申请号：US11291053

申请日：2005-11-30

申请人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

发明人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

IPC分类号： G01N33/48

CPC分类号： C12N1/06 ,  Y10T436/107497 ,  Y10T436/206664 ,  Y10T436/25 ,  Y10T436/2575

摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).

摘要翻译： 提供了使用自由基裂解细胞或病毒的方法。 该方法包括：向金属离子，过氧化物和细胞或病毒溶液的混合物施加电场以增加自由基产生，从而裂解细胞或病毒。 在本方法中，可以使用低电能（几mV至几V）有效地进行细胞裂解。 当本方法应用于微系统时，细胞裂解可以通过控制电能在期望的时间和期望的空间中发生，从而适于实现芯片上的实验室（LOC）。

公开(公告)号：US20060262642A1

公开(公告)日：2006-11-23

申请号：US11433594

申请日：2006-05-12

申请人： Chin-sung Park ,  Joon-ho Kim ,  Kak Namkoong ,  Kwang-wook Oh

发明人： Chin-sung Park ,  Joon-ho Kim ,  Kak Namkoong ,  Kwang-wook Oh

IPC分类号： B01F5/06

CPC分类号： B01F13/1013 ,  B01F5/0475 ,  B01F5/0478 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0059 ,  B01F13/1019 ,  B01F15/0203 ,  B01F15/0404 ,  B01F2015/0221 ,  G01N1/38

摘要： Provided is a fluid mixing device which produces a series of solutions with a concentration gradient. The fluid mixing device includes: a plurality of first channels disposed parallel to each other on a layer, and into which an equal amount of diluent flows from its upstream; a plurality of second channels formed perpendicular to the first channels on an adjacent layer to the layer on which the first channels are formed, and into which an equal amount of sample solution flows from its upstream; and via holes formed at at least one intersection between each of the first channel and a plurality of second channels so that a predetermined amount of sample solution flows from the second channels into corresponding first channels, wherein a series of solutions with different concentrations is produced in the first channels depending on the amount of sample solution that flows into the first channels through the via holes. Thus, a series of solutions with different concentrations is output from the first channels.

摘要翻译： 提供了一种流体混合装置，其产生具有浓度梯度的一系列溶液。 流体混合装置包括：在层上彼此平行设置的多个第一通道，等量的稀释剂从其上游流入其中; 多个第二通道，其垂直于与形成有第一通道的层相邻的第一通道形成，并且等量的样品溶液从其上游流入其中; 以及形成在每个第一通道和多个第二通道之间的至少一个相交处的通孔，使得预定量的样品溶液从第二通道流入相应的第一通道，其中一系列具有不同浓度的溶液在 第一通道取决于通过通孔流入第一通道的样品溶液的量。 因此，从第一通道输出一系列具有不同浓度的溶液。

公开(公告)号：US20060115902A1

公开(公告)日：2006-06-01

申请号：US11291053

申请日：2005-11-30

申请人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

发明人： Jung-im Han ,  Young-sun Lee ,  Kak Namkoong ,  Kwang-wook Oh

IPC分类号： G01N1/10

CPC分类号： C12N1/06 ,  Y10T436/107497 ,  Y10T436/206664 ,  Y10T436/25 ,  Y10T436/2575

摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).

公开(公告)号：US20060050277A1

公开(公告)日：2006-03-09

申请号：US11221055

申请日：2005-09-07

申请人： Gyeong-sik Ok ,  Jin-tae Kim ,  Kwang-wook Oh ,  Sang-hyo Kim

发明人： Gyeong-sik Ok ,  Jin-tae Kim ,  Kwang-wook Oh ,  Sang-hyo Kim

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01J3/51 ,  G01N2021/6421 ,  G01N2021/6471

摘要： Provided are an optical detection apparatus which can measure multi-channel samples at high speed and various wavelengths using an optical detector and a multi-channel sample analyzer employing the same. The optical detection apparatus includes an optical detector; a filter wheel having at least two color filters connected to each other in the shape of a disk; a plurality of optical channels through which a plurality of beams of light enter the filter wheel; and a mirror unit including a plurality of mirrors for sequentially reflecting the plurality of beams of light transmitted through the filter wheel to the optical detector, wherein the mirror unit rotates together with the filter wheel.

摘要翻译： 提供了一种光学检测装置，其可以使用光学检测器和使用该多通道样本的多通道样本分析仪来测量高速和各种波长的多通道样本。 光检测装置包括光检测器; 具有至少两个滤色器轮的滤光轮，所述至少两个滤色器以盘的形状彼此连接; 多个光通道，多个光束通过该光通道进入滤光轮; 以及镜单元，其包括多个反射镜，用于将透过滤光轮的多个光束顺序地反射到光学检测器，其中反射镜单元与滤光轮一起旋转。

公开(公告)号：US08167265B2

公开(公告)日：2012-05-01

申请号：US12968752

申请日：2010-12-15

申请人： Kwang-wook Oh ,  Kak Namkoong ,  Chin-sung Park

发明人： Kwang-wook Oh ,  Kak Namkoong ,  Chin-sung Park

IPC分类号： F16K31/00 ,  F16K31/18

CPC分类号： F15C5/00 ,  F16K99/0001 ,  F16K99/0032 ,  F16K99/0044 ,  F16K99/0046 ,  F16K2099/0084 ,  F16K2099/0086 ,  F16K2099/0092 ,  Y10T137/0391 ,  Y10T137/2191 ,  Y10T137/2196 ,  Y10T137/2224 ,  Y10T137/4643

摘要： Provided is a microvalve having a magnetic wax plug which includes a micro fluidic structure having an inlet portion and an outlet portion, a magnetic wax plug provided at a predetermined section where the inlet portion and the outlet portion meet, existing in a solid state, melted at a temperature higher than a predetermined temperature, and reversibly moving along a magnetic field, so as to control flux of a fluid through the micro fluidic structure, a heating portion provided corresponding to the section and heating the magnetic wax plug to be melted, and a magnetic field application portion selectively applying a magnetic field to a position where the melted magnetic wax plug arrives.

摘要翻译： 提供一种具有磁性蜡塞的微型阀，其包括具有入口部分和出口部分的微流体结构，设置在入口部分和出口部分相遇的预定部分处的固体状态的熔融蜡 在高于预定温度的温度下，并且沿着磁场可逆地移动，以便控制通过微流体结构的流体的通量;对应于该部分设置的加热部分，并加热待熔化的磁性蜡塞;以及 选择性地将磁场施加到熔融的磁性蜡塞到达的位置的磁场施加部。

公开(公告)号：US07928408B2

公开(公告)日：2011-04-19

申请号：US11345262

申请日：2006-02-01

申请人： Gyeong-sik Ok ,  Su-hyeon Kim ,  Jin-tae Kim ,  Kwang-wook Oh

发明人： Gyeong-sik Ok ,  Su-hyeon Kim ,  Jin-tae Kim ,  Kwang-wook Oh

IPC分类号： G01N21/64

CPC分类号： G01J3/10 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/0346 ,  G01N2021/3174 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484 ,  G01N2201/0221 ,  G01N2201/0407 ,  G01N2201/0461 ,  G01N2201/0612 ,  G01N2201/0627 ,  G01N2201/0631 ,  G01N2201/0806 ,  G01N2201/0833

摘要： A multi-channel fluorescence measuring optical system and a multi-channel fluorescence sample analyzer using the optical system are provided. The multi-channel fluorescence measuring optical system, which irradiates light onto a plurality of sample channels and detecting fluorescence radiated from samples, includes: a light source; an integrator for giving the light irradiated from the light source a uniform intensity distribution; a sample holder having a plurality of sample channels on which the samples are mounted, wherein the samples are exited by the light emitted from the integrator; and a beam splitter between the integrator and the sample holder for dividing the incident light in a predetermined ratio. Since the light intensities of fluorescence images are detected using optical fiber bundles and photodiodes, the manufacturing cost can be greatly reduced, and the optical system can be miniaturized.

摘要翻译： 提供多通道荧光测量光学系统和使用该光学系统的多通道荧光样品分析仪。 多通道荧光测量光学系统，其将光照射到多个样品通道上并检测从样品辐射的荧光，包括：光源; 用于给从光源照射的光均匀的强度分布的积分器; 具有多个样品通道的样品架，样品安装在样品通道上，其中采样由积分器发出的光退出; 以及在积分器和样品保持器之间的分束器，用于以预定比例分割入射光。 由于使用光纤束和光电二极管检测荧光图像的光强度，因此可以大大降低制造成本，并且可以使光学系统小型化。

公开(公告)号：US07892490B2

公开(公告)日：2011-02-22

申请号：US11391850

申请日：2006-03-29

申请人： Kwang-wook Oh ,  Yu-jin Seo

发明人： Kwang-wook Oh ,  Yu-jin Seo

IPC分类号： G01N21/00 ,  G01N31/00 ,  C12M1/36 ,  C12M1/38

CPC分类号： B01L3/502715 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2400/065

摘要： Provided is an apparatus for performing a chemical reaction using a microchip having at least one micro-channel. The device, which is a semiautomatic operating device for a microchip on which at least one micro-channel with a reagent inlet is formed, includes: a base which accommodates the microchip; a slider with injection inlets corresponding to the reagent inlets that reciprocally move parallel to the base; and a slider moving unit which selectively moves the slider to a first location at which the microchip is opened, after the injection inlet of the slider and the reagent inlet are aligned, and to a second location where the microchip is sealed by a bottom surface of the slider covering the reagent inlet.

摘要翻译： 提供一种使用具有至少一个微通道的微芯片进行化学反应的装置。 该装置是其上形成有至少一个具有试剂入口的微通道的微芯片的半自动操作装置，包括：容纳微芯片的基座; 具有与所述试剂入口相对应的注射入口的滑块，所述试剂入口与所述底座平行地往复移动; 以及滑块移动单元，其在滑块和试剂入口的注入口对齐之后，将滑块选择性地移动到微芯片打开的第一位置，并且在第二位置，微芯片被底部表面密封 滑块覆盖试剂入口。

公开(公告)号：US07371530B2

公开(公告)日：2008-05-13

申请号：US11066910

申请日：2005-02-25

申请人： Jung-im Han ,  Kwang-wook Oh

发明人： Jung-im Han ,  Kwang-wook Oh

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/508 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0645 ,  C12Q2565/607 ,  C12Q2565/537 ,  C12Q2561/113 ,  C12Q2563/155

摘要： Provided is a micro PCR device. The micro PCR device includes a PCR chamber having electrodes. First primers of PCR primer sets are immobilized on the surfaces of the electrodes and second primers labeled with nanoparticles of the PCR primer sets are added to the PCR chamber.

摘要翻译： 提供了一种微型PCR装置。 微PCR装置包括具有电极的PCR室。 将PCR引物组的第一引物固定在电极的表面上，并将PCR引物组的纳米颗粒标记的第二引物加入到PCR室中。

公开(公告)号：US20060286582A1

公开(公告)日：2006-12-21

申请号：US11430741

申请日：2006-05-09

申请人： Kwang-wook Oh ,  Yu-jin Seo

发明人： Kwang-wook Oh ,  Yu-jin Seo

IPC分类号： C12Q1/68

CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/043 ,  B01L2300/0816 ,  B01L2400/0406 ,  G01N2035/00158

摘要： Provided is a microchip assembly including a base holder, a microchip installed on the base holder and having at least one input hole into which a reaction sample is input and at least one microchamber accommodating the input reaction sample, a flipper rotatably installed at the outside of the microchip on the base holder and opening and closing the input hole of the microchip while flipping up in a vertically erected state or flipping down in a horizontal state, and slider installed at the outside of the flipper on the base holder to be capable of sliding and supporting the flipper in the erected state and flipping down the flipper while moving inside.

摘要翻译： 提供了一种微芯片组件，其包括基座，安装在基座上的微芯片，并具有输入反应样品的至少一个输入孔和容纳该输入反应样品的至少一个微室， 该微型芯片位于基座上，并且在垂直竖立状态下翻转或以水平状态翻转时打开和关闭微芯片的输入孔，以及滑块安装在底架上的导板外侧以能够滑动 并且在竖立状态下支撑导板，并在向内移动的情况下翻转导板。

公开(公告)号：US20060160688A1

公开(公告)日：2006-07-20

申请号：US11333857

申请日：2006-01-17

申请人： Kak Namkoong ,  Young-sun Lee ,  Kwang-wook Oh ,  Christopher Ko ,  Jung-Im Han

发明人： Kak Namkoong ,  Young-sun Lee ,  Kwang-wook Oh ,  Christopher Ko ,  Jung-Im Han

IPC分类号： B04B5/02

CPC分类号： B01L3/5021 ,  B01L2300/043 ,  B01L2300/0681 ,  B04B5/0414 ,  B04B9/00 ,  B04B9/12

摘要： A handheld centrifuge is provided. In the handheld centrifuge, an inertia body has an axle to which one end of a string is connected and a pair of inertia wheels coupled to the axle, and at least one closed vessel is installed at the inertia body to contain a substance to be centrifuged.

摘要翻译： 提供手持离心机。 在手持式离心机中，惯性体具有一个轴的一端连接到该轴上，一对惯性轮联接到轴上，并且至少一个密封容器安装在惯性体上以容纳要离心的物质 。