公开(公告)号：US20230372926A1

公开(公告)日：2023-11-23

申请号：US18227218

申请日：2023-07-27

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Lorenzo Franco Teodoro Petrini ,  Iago Pereiro Pereiro ,  Robert Dean Lovchik ,  Anna Fomitcheva Khartchenko ,  Anel Zulji ,  Govind Kaigala ,  Cristina Boero ,  Olivier Michel Christian Noël ,  Christophe Guex ,  Stéphane Guillod ,  Aurélie Bolon

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L13/02 ,  B01L2300/14 ,  B01L2200/0642

Abstract: A method comprises providing a microfluidic device including a reservoir; an injection nozzle at the bottom of the reservoir, the injection nozzle being wider at its top than at its bottom; an injection channel below the injection nozzle; and a microfluidic channel below the injection channel. The method further comprises placing fluid in the reservoir, and allowing the fluid to passively flow through the injection nozzle and the injection channel.

公开(公告)号：US20230340627A1

公开(公告)日：2023-10-26

申请号：US18296357

申请日：2023-04-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Polina TSIGANKOV ,  Dalia SHEZIFI ,  Galina FIHMAN ,  Matt DUNN ,  Brigid CUNNION

IPC: C12Q1/70 ,  G01N33/569

CPC classification number: C12Q1/701 ,  G01N33/56983 ,  C12Q1/6851

Abstract: Methods, compositions, and kits for analyzing viral particles. In an exemplary method of analyzing viral particles, capsids of the viral particles may be tagged with a tag. Subsamples of a sample containing the viral particles may be formed. Each subsample of only a subset of the subsamples may include at least one of the viral particles. One or more targets may be amplified from a genome of the viral particles. Tag-related data, and amplification data for the one or more targets, may be collected from the subsamples. In some examples, a capsid occupancy of the viral particles may be determined in a calibration-free approach using the collected data without quantification of the viral particles or capsids thereof.

公开(公告)号：US20230323435A1

公开(公告)日：2023-10-12

申请号：US18185220

申请日：2023-03-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Vishal SHARMA ,  Ronald LEBOFSKY

IPC: C12Q1/6837 ,  C12N15/10

CPC classification number: C12Q1/6837 ,  C12N15/1065

Abstract: The methods allow for provision of a mixture of a plurality of beads, each bead linked to oligonucleotides, wherein the mixture can be treated as a bulk solution (prior to partitioning) to cleave a covalent bond linking the oligonucleotides to the beads while retaining a non-covalent linkage (via hybridization) between the beads and the oligonucleotides, allowing for distribution of the oligonucleotides and beads to partitions or 2D arrays prior to separation of the oligonucleotides from the beads, which occurs for example in the partitions.

公开(公告)号：US11759780B2

公开(公告)日：2023-09-19

申请号：US17153311

申请日：2021-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Joshua Ritchey ,  Yi Xue ,  Robert Meltzer

IPC: B01L3/00 ,  C09D183/04 ,  C09D183/00 ,  B81C1/00 ,  C12Q1/686

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502784 ,  B81C1/00206 ,  C09D183/00 ,  C09D183/04 ,  B01L2200/06 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/04 ,  C12Q1/686 ,  C09D183/04 ,  C08K3/36

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

公开(公告)号：US11759761B2

公开(公告)日：2023-09-19

申请号：US17381096

申请日：2021-07-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Nicholas Heredia

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q2525/131 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2523/319 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519 ,  C12Q1/6806 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

公开(公告)号：US11739307B2

公开(公告)日：2023-08-29

申请号：US17055801

申请日：2019-05-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US20230194517A1

公开(公告)日：2023-06-22

申请号：US18083131

申请日：2022-12-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Eli HEFNER

IPC: G01N33/543 ,  G01N21/64 ,  B01L3/00

CPC classification number: G01N33/54386 ,  G01N21/6428 ,  G01N21/6458 ,  B01L3/502761 ,  G01N2021/6439 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2200/0652

Abstract: Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.

公开(公告)号：US11666888B2

公开(公告)日：2023-06-06

申请号：US16967429

申请日：2019-02-05

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Christopher Belisle ,  Hong Chen ,  Yueping Xu ,  Jiali Liao ,  Xuemei He

IPC: B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/289 ,  B01J20/32 ,  C07K16/06 ,  B01J20/26

CPC classification number: B01J20/262 ,  B01D15/363 ,  B01J20/289 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3227 ,  B01J20/3248 ,  B01J20/3253 ,  B01J20/3285 ,  C07K16/065 ,  B01D15/327 ,  B01D15/3847

Abstract: Chromatography resins having mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US20230152011A1

公开(公告)日：2023-05-18

申请号：US17989938

申请日：2022-11-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir SADRI ,  Nenad KIRCANSKI ,  Thanh-Vi TRAN ,  Carl MARLOWE ,  Brian David WILSON

IPC: F25B21/04 ,  B01L7/00 ,  H10N10/10 ,  H10N10/80

CPC classification number: F25B21/04 ,  B01L7/00 ,  B01L7/52 ,  H10N10/10 ,  H10N10/80 ,  B01L2300/1805 ,  B01L2300/08 ,  B01L2300/123

Abstract: A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.

公开(公告)号：US11639933B2

公开(公告)日：2023-05-02

申请号：US16141069

申请日：2018-09-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Boaz Gildor ,  Dalia Shezifi

IPC: G01N33/543 ,  G01N33/541

Abstract: Methods of detecting a target in a sample are provided. Kits for performing the methods described herein are also provided.