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    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity
    34.
    发明授权
    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity 失效
    具有生物识别特异性的重组链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白

    公开(公告)号：US06391590B1

    公开(公告)日：2002-05-21

    申请号：US07780717

    申请日：1991-10-21

    Applicant: Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    Inventor： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    IPC: C12N1562

    CPC classification number: C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

    Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

    Abstract translation: 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

    Sequence directed DNA binding molecules compositions and methods
    35.
    发明授权
    Sequence directed DNA binding molecules compositions and methods 失效
    序列定向DNA结合分子的组成和方法

    公开(公告)号：US06384208B1

    公开(公告)日：2002-05-07

    申请号：US09354947

    申请日：1999-07-15

    Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

    Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

    IPC: C07H2104

    CPC classification number: C12Q1/701 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6811 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/705 ,  H01L2924/14 ,  C12Q2525/161 ,  C12Q2525/131 ,  C12Q2537/161 ,  C12Q2522/101 ,  C12Q2521/301

    Abstract: The present invention defines a DNA: protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

    Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

    Sequence-directed DNA-binding molecules compositions and methods
    36.
    发明授权
    Sequence-directed DNA-binding molecules compositions and methods 失效
    序列指导的DNA结合分子的组成和方法

    公开(公告)号：US5738990A

    公开(公告)日：1998-04-14

    申请号：US475221

    申请日：1995-06-07

    Applicant: Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

    Inventor： Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

    IPC: C07K14/47 ,  C12N15/09 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  C12P21/02 ,  C07H21/04

    CPC classification number: C12N15/67 ,  C07K14/47 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6883 ,  C12Q1/705

    Abstract: The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

    Abstract translation: 本发明定义了一种用于筛选合成或生物化合物文库以测定其结合特异性DNA测试序列的能力的测定法。 该测定还可用于确定DNA结合分子对于任何特定DNA序列的序列特异性和相对DNA结合亲和力。 本文还描述了测定的潜在应用，包括：1）通过大量筛选合成或生物化合物（即发酵液）文库来检测铅化合物或新药物; 2）由使用该测定法确定序列特异性的分子的同源或异源亚基组成的序列特异性DNA结合药物的设计; 和3）使用通过测定作为共价连接的部分确定序列特异性的分子，以有助于核酸或其它大分子聚合物与核酸序列的结合。

    Method of ordering sequence binding preferences of a DNA-binding molecule
    37.
    发明授权
    Method of ordering sequence binding preferences of a DNA-binding molecule 失效
    排序DNA结合分子的序列结合偏好的方法

    公开(公告)号：US5693463A

    公开(公告)日：1997-12-02

    申请号：US996783

    申请日：1992-12-23

    Applicant: Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

    Inventor： Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

    IPC: C07K14/47 ,  C12N15/09 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/02 ,  C12N15/00 ,  G01N33/574

    CPC classification number: C12N15/67 ,  C07K14/47 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6883 ,  C12Q1/705

    Abstract: The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

    Abstract translation: 本发明定义了一种用于筛选合成或生物化合物文库以测定其结合特异性DNA测试序列的能力的测定法。 该测定还可用于确定DNA结合分子对于任何特定DNA序列的序列特异性和相对DNA结合亲和力。 本文还描述了测定的潜在应用，包括：1）通过大量筛选合成或生物化合物（即发酵液）文库来检测铅化合物或新药物; 2）由使用该测定法确定序列特异性的分子的同源或异源亚基组成的序列特异性DNA结合药物的设计; 和3）使用通过测定作为共价连接的部分确定序列特异性的分子，以有助于核酸或其它大分子聚合物与核酸序列的结合。

    Immuno-polymerase chain reaction system for antigen detection
    39.
    发明授权
    Immuno-polymerase chain reaction system for antigen detection 失效
    抗原检测免疫聚合酶链反应体系

    公开(公告)号：US5665539A

    公开(公告)日：1997-09-09

    申请号：US131301

    申请日：1993-10-04

    Applicant: Takeshi Sano ,  Charles R. Cantor ,  Cassandra L. Smith

    Inventor： Takeshi Sano ,  Charles R. Cantor ,  Cassandra L. Smith

    IPC: A61K38/00 ,  A61K47/48 ,  C07K14/31 ,  C07K14/36 ,  C12Q1/68 ,  C12Q1/66

    CPC classification number: C07K14/31 ,  B82Y5/00 ,  C07K14/36 ,  A61K38/00

    Abstract: A novel system and method for sensitive antigen detection. The system utilizes immuno-polymerase chain reaction in which a specific biotinylated nucleic acid molecule is used as the marker. The biotinylated marker is attached to antigen-antibody complex through a streptavidin-protein A chimeric protein that possesses tight and specific binding affinity both for biotin and immunoglobulin G. A segment of the attached biotinylated marker is amplified by polymerase chain reactions with appropriate primers and the polymerase chain reaction products are detected by agarose gel electrophoresis. The method can detect any antigen and has a greater sensitivity than any existing antigen detection system.

    Abstract translation: 一种用于敏感抗原检测的新型系统和方法。 该系统利用免疫聚合酶链反应，其中使用特异性生物素化的核酸分子作为标记。 生物素标记通过链霉抗生物素蛋白 - 蛋白A嵌合蛋白连接到抗原 - 抗体复合物上，该嵌合蛋白对于生物素和免疫球蛋白G都具有紧密和特异性的结合亲和力。通过与适当引物的聚合酶链反应扩增连接的生物素标记的片段，并且 通过琼脂糖凝胶电泳检测聚合酶链反应产物。 该方法可以检测任何抗原并且比任何现有的抗原检测系统具有更高的灵敏度。

    Design and synthesis of bispecific DNA-antibody conjugates
    40.
    发明授权
    Design and synthesis of bispecific DNA-antibody conjugates 失效
    双特异性DNA抗体缀合物的设计与合成

    公开(公告)号：US5635602A

    公开(公告)日：1997-06-03

    申请号：US107186

    申请日：1993-08-13

    Applicant: Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    Inventor： Charles R. Cantor ,  Roy S. Chuck ,  Doris B. Tse

    IPC: C07K16/28 ,  C07K16/46 ,  C07K17/00 ,  C07K19/00

    CPC classification number: C07K16/2812 ,  C07K16/2809 ,  C07K16/468

    Abstract: The invention relates to bis-protein-DNA conjugates. A protein having an antigen specific binding activity is covalently linked to each end of a derivatized DNA molecule. The bis-protein-DNA conjugates can be used for immunoassays and measuring distances between proteins at up to 3.4 .ANG. resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be indirectly conjugated to an antibody or Fab' fragment, using a avidin/streptavidin-biotin linkage. The conjugates of the invention can be used in immunoassays and PCR assays.

    Abstract translation: 本发明涉及双蛋白-DNA缀合物。 具有抗原特异性结合活性的蛋白质与衍生的DNA分子的每个末端共价连接。 双蛋白-DNA缀合物可用于免疫测定，并以高达3.4 ANGSTROM分辨率测量蛋白质之间的距离。 本发明还涉及合成这些双蛋白-DNA缀合物的方法。 共轭物的合成需要衍生DNA寡核苷酸的5'或3'末端并共价连接该DNA与蛋白质。 可以使用抗生物素蛋白/链霉抗生物素蛋白 - 生物素连接，将DNA间接缀合至抗体或Fab'片段。 本发明的缀合物可用于免疫测定和PCR测定。

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