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    UNIVERSAL PROBE ASSAY METHODS
    66.
    发明申请
    UNIVERSAL PROBE ASSAY METHODS 审中-公开
    通用检测方法

    公开(公告)号：US20160348149A1

    公开(公告)日：2016-12-01

    申请号：US15144529

    申请日：2016-05-02

    Applicant: Fluidigm Corporation

    Inventor： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC: C12Q1/68

    CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物 。

    SINGLE-CELL NUCLEIC ACID ANALYSIS
    67.
    发明申请
    SINGLE-CELL NUCLEIC ACID ANALYSIS 审中-公开
    单细胞核酸分析

    公开(公告)号：US20160340728A1

    公开(公告)日：2016-11-24

    申请号：US14977414

    申请日：2015-12-21

    Applicant: Fluidigm Corporation

    Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

    IPC: C12Q1/68

    CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

    Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

    Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。

    NUCLEOTIDE ANALOGS
    69.
    发明申请
    NUCLEOTIDE ANALOGS 审中-公开
    核素类似物

    公开(公告)号：US20160168636A1

    公开(公告)日：2016-06-16

    申请号：US14882866

    申请日：2015-10-14

    Applicant: Fluidigm Corporation

    Inventor： Suhaib Siddiqi ,  J. William Efcavitch ,  Judith Mitchell ,  Subramanian Marappan ,  Jayson Bowers ,  Mirna Jaroza

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  A61K49/0041 ,  A61K49/0052 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/6869 ,  C12Q2563/107

    Abstract: The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-G, 9-deaza-A, or ψ-uridine.

    Abstract translation: 本发明一般涉及核苷酸类似物及其在合成反应测序中的用途。 在某些实施方案中，本发明提供核苷酸类似物，其包括通过可切割接头连接到核苷酸类似物的含氮碱基部分的可检测标记，其中所述类似物与至少一种活化剂的接触导致标记的切割和消除 接头，从而产生天然核苷酸，9-脱氮-G，9-脱氮-A或ψ-尿苷。

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