公开(公告)号：US09377455B2

公开(公告)日：2016-06-28

申请号：US14746276

申请日：2015-06-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20150174578A1

公开(公告)日：2015-06-25

申请号：US14590470

申请日：2015-01-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zishan Hua ,  Arjun Sudarsan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

Abstract translation: 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。

公开(公告)号：US20150021189A1

公开(公告)日：2015-01-22

申请号：US14455645

申请日：2014-08-08

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Michael G. Pollack

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0448

Abstract: A droplet actuator configured to improve the throughput of droplet operations in a detection spot of the droplet actuator and/or to reduce carryover problems is provided. The droplet actuator may include electrodes configured for effecting droplet operations transporting droplets on a surface; a sensor arranged in proximity to one or more of the electrodes establishing a detection window on the surface for detection of one or more properties of one or more droplets on the surface; wherein the electrodes may establish at least two pathways for transport of droplets into the detection window.

Abstract translation: 提供了一种配置成提高液滴致动器的检测点中的液滴操作的吞吐量和/或减少携带问题的液滴致动器。 液滴致动器可以包括被配置为用于实现在表面上传送液滴的液滴操作的电极; 设置在一个或多个电极附近的传感器，在表面上建立检测窗口，以检测表面上的一个或多个液滴的一个或多个特性; 其中电极可以建立用于将液滴输送到检测窗中的至少两个路径。

公开(公告)号：US20140332380A1

公开(公告)日：2014-11-13

申请号：US14338889

申请日：2014-07-23

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2400/0427 ,  G01N27/22 ,  G01N27/223 ,  G01N27/227 ,  G01N2203/00 ,  G01V3/02

Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

Abstract translation: 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。

公开(公告)号：US20160114320A1

公开(公告)日：2016-04-28

申请号：US14935823

申请日：2015-11-09

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

公开(公告)号：US20150336098A1

公开(公告)日：2015-11-26

申请号：US14817834

申请日：2015-08-04

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00

CPC classification number: G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

Abstract translation: 公开了用于操纵液滴的装置和方法。 在一个实施例中，提供了一种用于操纵液滴的装置，该装置包括一个基板，设置在该基板上的多个电极阵列，其中每个阵列中的对应电极连接到公共电信号，以及首先设置在基板上的电介质层 侧表面并图案化以覆盖电极。

公开(公告)号：US09180450B2

公开(公告)日：2015-11-10

申请号：US14446733

申请日：2014-07-30

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: G01N27/453 ,  B01L3/00 ,  B01F11/00 ,  B01F13/00 ,  G02B26/00 ,  C25B9/00 ,  B41J2/14

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。

公开(公告)号：US20150060284A1

公开(公告)日：2015-03-05

申请号：US14538322

申请日：2014-11-11

Applicant: DUKE UNIVERSITY

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: G01N27/447 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/68

CPC classification number: G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

公开(公告)号：US08871071B2

公开(公告)日：2014-10-28

申请号：US13759416

申请日：2013-02-05

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B Fair

IPC: G01N27/447 ,  G01N27/453 ,  C25B9/00 ,  B01L3/00 ,  B01F13/00 ,  B01F11/00 ,  G02B26/00 ,  B41J2/14

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。

公开(公告)号：US10022719B2

公开(公告)日：2018-07-17

申请号：US14935823

申请日：2015-11-09

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: G01N27/447 ,  B01L3/00 ,  B01F11/00 ,  B01F13/00 ,  G02B26/00 ,  C25B9/00 ,  B41J2/14

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.