公开(公告)号：US08962279B2

公开(公告)日：2015-02-24

申请号：US12655459

申请日：2009-12-30

申请人： David J. Liu ,  Xing Su ,  Kai Wu

发明人： David J. Liu ,  Xing Su ,  Kai Wu

IPC分类号： C12P19/34 ,  C12M1/00 ,  C12Q1/48 ,  C12Q1/68 ,  G01N27/414

CPC分类号： C12Q1/48 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2333/9125 ,  C12Q2565/607 ,  C12Q2565/507 ,  C12Q2565/301

摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.

摘要翻译： 介绍了核酸测序方法。 通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。 在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸， 切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。 焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。 另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

公开(公告)号：US08920564B2

公开(公告)日：2014-12-30

申请号：US13169373

申请日：2011-06-27

申请人： Gwo-Chuan Tzu ,  Xiaoxiong Yuan ,  Amit Khandelwal ,  Benjamin Cheng Wang ,  Avgerinos V. Gelatos ,  Kai Wu ,  Michael P. Karazim ,  Jing Lin ,  Olkan Cuvalci

发明人： Gwo-Chuan Tzu ,  Xiaoxiong Yuan ,  Amit Khandelwal ,  Benjamin Cheng Wang ,  Avgerinos V. Gelatos ,  Kai Wu ,  Michael P. Karazim ,  Jing Lin ,  Olkan Cuvalci

IPC分类号： C23C16/00 ,  C23C16/44 ,  C23C16/455 ,  C23C16/52 ,  C23C16/458 ,  C23C16/46

CPC分类号： C23C16/4412 ,  C23C16/45565 ,  C23C16/45587 ,  C23C16/4585 ,  C23C16/46 ,  C23C16/52

摘要： A substrate support may include a body; an inner ring disposed about the body; an outer ring disposed about the inner ring forming a first opening therebetween; a first seal ring disposed above the first opening; a shadow ring disposed above the inner ring, extending inward from the outer ring and forming a second opening between the shadow and outer rings; a second seal ring disposed above the second opening; a space at least partially defined by the body and the inner, outer, first, second, and shadow rings; a first gap defined between a processing surface of a substrate when present and the shadow ring; and a plurality of second gaps fluidly coupled to the space; wherein the first gap and the plurality of second gaps are configured such that, when a substrate is present, a gas provided to the space flows out of the space through the first gap.

摘要翻译： 衬底支撑件可以包括主体; 围绕身体设置的内圈; 围绕所述内圈设置的外圈，在其间形成第一开口; 设置在所述第一开口上方的第一密封环; 设置在所述内圈上方的从所述外圈向内延伸并在所述阴影和外圈之间形成第二开口的阴影环; 设置在所述第二开口上方的第二密封环; 至少部分地由身体和内，外，第一，第二和第二和阴影环限定的空间; 限定在衬底的处理表面和阴影环之间的第一间隙; 以及流体耦合到所述空间的多个第二间隙; 其中所述第一间隙和所述多个第二间隙被配置为使得当存在基板时，设置到所述空间的气体通过所述第一间隙流出所述空间。

公开(公告)号：US20140342470A1

公开(公告)日：2014-11-20

申请号：US14448141

申请日：2014-07-31

申请人： Xing Su ,  David J. Liu ,  Kai Wu ,  Kenneth B. Swartz ,  Mineo Yamakawa

发明人： Xing Su ,  David J. Liu ,  Kai Wu ,  Kenneth B. Swartz ,  Mineo Yamakawa

IPC分类号： G01N1/40

CPC分类号： G01N1/4044 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0638 ,  B01L2400/086 ,  G01N35/0098 ,  G01N35/08 ,  G01N2015/0038 ,  G01N2015/0092 ,  H01F7/20 ,  H01F7/206 ,  H01F2007/068 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T29/49117 ,  Y10T29/4913 ,  Y10T29/49155 ,  Y10T436/25

摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

摘要翻译： 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

公开(公告)号：US08715932B2

公开(公告)日：2014-05-06

申请号：US12860462

申请日：2010-08-20

申请人： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

发明人： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q2531/125 ,  C12Q2535/122 ,  C12Q2565/301 ,  C12Q2565/607

摘要： Nucleic acid sequencing using concatemers of DNA is provided. Optionally, amplified reaction products from the repeated incorporation and excision of a nucleoside complementary to a nucleoside of the DNA to be sequenced onto primer molecules hybridized to the concatemers of DNA are detected. Nucleic acid sequencing using concatemers of DNA and non-natural oligonucleotides is also provided. Nucleic acid sequencing reactions are detected electronically and or optically using arrays of detectors.

摘要翻译： 提供使用DNA连接子的核酸测序。 任选地，检测来自重复掺入和切除与待测序的DNA的核苷互补的核苷酸的扩增反应产物与DNA连接体杂交的引物分子上。 还提供了使用DNA和非天然寡核苷酸的并联体的核酸测序。 使用检测器阵列电子和/或光学检测核酸测序反应。

公开(公告)号：US08674086B2

公开(公告)日：2014-03-18

申请号：US12823995

申请日：2010-06-25

申请人： Jianquan Liu ,  Xing Su ,  Kai Wu

发明人： Jianquan Liu ,  Xing Su ,  Kai Wu

IPC分类号： C07H19/20 ,  C07H19/00 ,  C07H19/04

CPC分类号： C07H19/10 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2521/319 ,  C12Q2521/525 ,  C12Q2525/186 ,  C12Q2537/157 ,  C12Q2565/537 ,  C12Q2565/607

摘要： Embodiments of the invention provide non-natural bifunctional nucleotides having both nuclease resistance and nucleic acid synthesis blocking properties and methods of sequencing nucleic acids that employ non-natural bifunctional nucleic acids. Additional embodiments provide non-natural oligonucleotides and methods for sequencing nucleic acids using the non-natural oligonucleotides. Methods according to embodiments of the invention employ electronic detection and fluorescent detection of nucleic acid sequencing reactions.

摘要翻译： 本发明的实施方案提供具有核酸酶抗性和核酸合成阻断性质的非天然双功能核苷酸，以及使用非天然双功能核酸测序核酸的方法。 另外的实施方案提供非天然寡核苷酸和使用非天然寡核苷酸测序核酸的方法。 根据本发明的实施方案的方法使用核酸测序反应的电子检测和荧光检测。

公开(公告)号：US20140038587A1

公开(公告)日：2014-02-06

申请号：US13563855

申请日：2012-08-01

申请人： Tudor Murgan ,  Kai Wu ,  Stefan Herzinger ,  Christian Drewes ,  Jean-Xavier Canonici ,  Bertram Gunzelmann

发明人： Tudor Murgan ,  Kai Wu ,  Stefan Herzinger ,  Christian Drewes ,  Jean-Xavier Canonici ,  Bertram Gunzelmann

IPC分类号： H04W24/00 ,  H04W88/02

CPC分类号： H04W48/16

摘要： A method for searching for a radio cell is provided comprising determining, for each of a plurality of frequencies, a reception strength value indicating the reception strength within a first bandwidth around the frequency, wherein the first bandwidth is smaller than a second bandwidth with which a radio cell to be searched is operated; determining, based on the determined reception strength values, a spectral position for a radio cell at which a cell search is to be carried out; and carrying out a cell search at the determined spectral position.

摘要翻译： 提供了一种用于搜索无线电小区的方法，包括为多个频率中的每一个确定指示频率周围的第一带宽内的接收强度的接收强度值，其中第一带宽小于第二带宽， 操作要搜索的无线电信元; 基于所确定的接收强度值，确定要进行小区搜索的无线电小区的频谱位置; 并在确定的光谱位置执行小区搜索。

公开(公告)号：US20140001055A1

公开(公告)日：2014-01-02

申请号：US13538346

申请日：2012-06-29

申请人： Oguz H. Elibol ,  Grace M. Credo ,  Xing Su ,  Madoo Varma ,  Jonathan S. Daniels ,  Drew Hall ,  Handong Li ,  Noureddine Tayebi ,  Kai Wu

发明人： Oguz H. Elibol ,  Grace M. Credo ,  Xing Su ,  Madoo Varma ,  Jonathan S. Daniels ,  Drew Hall ,  Handong Li ,  Noureddine Tayebi ,  Kai Wu

IPC分类号： G01N27/26 ,  B82Y5/00 ,  B82Y30/00

CPC分类号： G01N27/3277 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00653 ,  B01J2219/00722 ,  B81B7/04 ,  B81B2201/0214 ,  B81B2201/058 ,  B82Y15/00 ,  C40B20/04 ,  C40B50/16 ,  G01N27/3278 ,  G01N27/4145 ,  G01N27/4146 ,  G01N27/4148

摘要： Various embodiments provide devices, methods, and systems for high throughput biomolecule detection using transducer arrays. In one embodiment, a transducer array made up of transducer elements may be used to detect byproducts from chemical reactions that involve redox genic tags. Each transducer element may include at least a reaction chamber and a fingerprinting region, configured to flow a fluid from the reaction chamber through the fingerprinting region. The reaction chamber can include a molecule attachment region and the fingerprinting region can include at least one set of electrodes separated by a nanogap for conducting redox cycling reactions. In embodiments, by flowing the chamber content obtained from a reaction of a latent redox tagged probe molecule, a catalyst, and a target molecule in the reaction chamber through the fingerprinting region, the redox cycling reactions can be detected to identify redox-tagged biomolecules.

摘要翻译： 各种实施方案提供使用换能器阵列进行高通量生物分子检测的装置，方法和系统。 在一个实施例中，由换能器元件组成的换能器阵列可用于检测涉及氧化还原基因标签的化学反应的副产物。 每个换能器元件可以至少包括反应室和指纹区域，其被配置为使来自反应室的流体通过指纹区域流动。 反应室可以包括分子附着区域，并且指纹区域可以包括由纳米间隙分开的至少一组电极，用于进行氧化还原循环反应。 在实施方案中，通过使反应室中潜氧氧化还原标记的探针分子，催化剂和靶分子的反应通过指纹区域流动所述腔室内容物，可以检测氧化还原循环反应以鉴定氧化还原标记的生物分子。

公开(公告)号：US20130270353A1

公开(公告)日：2013-10-17

申请号：US13809889

申请日：2011-12-31

申请人： Hongyu Long ,  Kai Wu ,  Yulong Yang

发明人： Hongyu Long ,  Kai Wu ,  Yulong Yang

IPC分类号： F24H9/20 ,  F24H4/00

CPC分类号： F24H9/2007 ,  F02C3/28 ,  F02C6/18 ,  F24D15/04 ,  F24D19/1039 ,  F24D19/1048 ,  F24D2200/12 ,  F24H4/00 ,  F24H2240/00 ,  H02J3/381 ,  H02J3/383 ,  Y02B10/20 ,  Y02B10/70 ,  Y02B30/12 ,  Y02E10/563 ,  Y02E20/14

摘要： The present invention provides a joint heating system of gas combined cycle and solar power and a dispatching method thereof, the user adopts two ways of the hot water radiator and the heat pump to supply heat, wherein the hot water comes from the gas combined cycle units, the electric power comes from the combination of the gas combined cycle units and the solar power generation units, and after detecting the power supplying and power consumption of the user in a historical time period by the comprehensive dispatching and controlling device, a future time period is predicted, and then dispatching is processed on the basis, under the circumstances of ensuring fulfilling of electric power supply and heat supply, reducing of the heating hot water flow output is compensated by consuming electric power to heat, consuming electric power to heat can not only compensate the shortage of hot water heating, but also increases the load in power trough time; accordingly, solar power generation, and combined production of heat and power is synthesized, the output of the combined production of heat and power is adjusted according to the volatility of solar power generation, and further according to change of power consumption load of the user, based on continuous dispatching method of real-time detection and prediction, with equal detection period and adjustment period, an equivalent smooth output of solar power generation on the user side is realized.

摘要翻译： 本发明提供一种气体联合循环和太阳能发电联合加热系统及其调度方法，用户采用热水散热器和热泵两种方式供热，其中热水来自气体联合循环装置 电力来自气体联合循环单元和太阳能发电单元的组合，并且在由综合调度和控制装置在历史时间段内检测到用户的供电和功耗之后，未来的时间段 在此基础上进行调度处理，在确保电力供热供热的情况下，通过消耗电力加热来补偿加热热水流量输出的减少，消耗电热不能 只补偿热水供热不足，同时也增加了供电时间的负荷; 因此，合成太阳能发电和热电联产，根据太阳能发电的波动调整热电联产产量，进一步根据用户的功耗负荷变化， 基于实时检测和预测的连续调度方法，具有相同的检测周期和调整周期，实现了用户端太阳能发电的相当平滑输出。

公开(公告)号：US08355232B2

公开(公告)日：2013-01-15

申请号：US13268297

申请日：2011-10-07

申请人： Kai Wu

发明人： Kai Wu

IPC分类号： H02H5/04

CPC分类号： H02S40/34

摘要： The primary objective of the present invention is to provide a solar module with a bimetal breaker, which comprises a solar module with terminal ribbons deposited on both sides of the solar module and inter-connecting ribbons extended and connected to the terminal ribbons, and a bimetal breaker being deposited on a joint of the terminal ribbon and the inter-connecting ribbon of the solar module. The bimetal breaker comprises a bimetal unit that is composed of two metal layers, and when the bimetal unit is heated, the bimetal unit will bend because of different thermal expansion coefficients and push the conducting terminals to separate from each other, so as to prevent the solar module from being damaged due to high temperature.

摘要翻译： 本发明的主要目的是提供一种具有双金属断路器的太阳能模块，其包括太阳能模块，其具有沉积在太阳能模块两侧的端子带和延伸并连接到端子带的互连带和双金属片 断路器沉积在太阳能模块的端子带和连接带的接头上。 双金属断路器包括由两个金属层组成的双金属单元，并且当双金属单元被加热时，双金属单元将由于不同的热膨胀系数而弯曲，并且将导电端子彼此分离，以防止 太阳能组件由于高温而被损坏。

公开(公告)号：US20100167938A1

公开(公告)日：2010-07-01

申请号：US12319168

申请日：2008-12-31

申请人： Xing Su ,  Kai Wu ,  David J. Liu

发明人： Xing Su ,  Kai Wu ,  David J. Liu

IPC分类号： C40B30/00

CPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  C12Q2565/607 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2537/149 ,  C12Q2525/113 ,  C12Q2521/319

摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction regions containing sensors to monitor changes in solutions or bound molecules contained in the reaction regions. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction regions that allow for optical monitoring of solutions in the reaction regions. Chemical amplification schemes that allow DNA to be sequenced in which multiple nucleotide addition reactions are performed to detect the incorporation of a base are disclosed. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.

摘要翻译： 本发明的实施方案提供了使用包含传感器的反应区域的阵列来测序DNA的装置方法，以监测反应区域中包含的溶液或结合分子的变化。 另外的实施方案提供了使用允许对反应区域中的溶液进行光学监测的反应区域阵列来测序DNA的装置和方法。 公开了允许进行多核苷酸加成反应进行DNA测序以检测碱基掺入的化学扩增方案。 通过使用大阵列中包含的平行反应测序DNA，可以快速测序DNA。