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公开(公告)号:US20030077634A1
公开(公告)日:2003-04-24
申请号:US10228273
申请日:2002-08-27
IPC分类号: C12Q001/68 , G01N033/53 , G01N033/542 , C12M001/34
CPC分类号: G01N33/5091 , B01J19/0093 , B01L3/5025 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L9/527 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2200/0689 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0877 , B01L2300/0887 , B01L2400/0415 , G01N27/44791 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/5044 , G01N33/5064 , G01N33/5094 , G01N33/5304 , G01N33/57415 , G01N35/08 , Y10S366/02 , Y10S435/81 , Y10S435/97 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809
摘要: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
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公开(公告)号:US5972187A
公开(公告)日:1999-10-26
申请号:US45338
申请日:1998-03-20
申请人: J. Wallace Parce , Michael R. Knapp
发明人: J. Wallace Parce , Michael R. Knapp
IPC分类号: G01N31/20 , B01F13/00 , B01J19/00 , B01L3/00 , B01L3/02 , F04B17/00 , F04B19/00 , G01N27/26 , G01N27/447 , G01N37/00 , H02N11/00
CPC分类号: B01F13/0071 , B01J19/0093 , B01L3/502715 , B01L3/50273 , B01L3/502784 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44743 , G01N27/44791 , H02N11/006 , B01F13/0084 , B01F13/0086 , B01J2219/00891 , B01L2200/027 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L3/021 , Y10S366/02 , Y10T436/113332 , Y10T436/2575
摘要: The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.
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公开(公告)号:US5852495A
公开(公告)日:1998-12-22
申请号:US941679
申请日:1997-09-30
申请人: J. Wallace Parce
发明人: J. Wallace Parce
IPC分类号: G01N27/447 , G01N21/00
CPC分类号: G01N27/44791 , G01N27/44721 , Y10S366/02
摘要: The present invention provides a microfluidic system for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel in the microfluidic system are received by a photodetector. The intensity of light received by the photodetector is modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined and the identification of the species is made by its electrophoretic mobility in the channel.
摘要翻译: 本发明提供了一种用于在化学,生物化学,生物技术,分子生物学等许多领域中快速,准确和低成本电泳分析材料的微流体系统。 沿着微流体系统中的通道的周期性间隔的区域的光由光电检测器接收。 由光电探测器接收的光的强度通过电泳力通过通道的物种带的移动来调制。 通过傅立叶分析,确定每个物种带的速度,并通过其在通道中的电泳迁移率来确定物种的鉴定。
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公开(公告)号:US5800690A
公开(公告)日:1998-09-01
申请号:US678436
申请日:1996-07-03
IPC分类号: G01N27/26 , G01N27/447 , G01N37/00
CPC分类号: G01N27/44791 , G01N27/44713 , G01N27/44752 , G01N27/44773 , Y10S366/02 , Y10T29/49002
摘要: In a microfluidic system using electrokinetic forces, the present invention uses electrical current or electrical parameters, other than voltage, to control the movement of fluids through the channels of the system. Time-multiplexed power supplies also provide further control over fluid movement by varying the voltage on an electrode connected to a fluid reservoir of the microfluidic system, by varying the duty cycle during which the voltage is applied to the electrode, or by a combination of both. A time-multiplexed power supply can also be connected to more than one electrode for a savings in cost.
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公开(公告)号:US20040034513A1
公开(公告)日:2004-02-19
申请号:US10443657
申请日:2003-05-22
发明人: Steven J. Gallagher
IPC分类号: G06F017/50
CPC分类号: G01N30/86 , G01N27/44717 , G01N30/8651 , G01N2030/625
摘要: Techniques for displaying chromatographic data using a graphical user interface are provided. Chromatographic separation data that is a series of measurements for a sample at a scanning location over time can be displayed on a display device in a series of bands. Additionally, the series of bands for multiple samples can be aligned on the display device.
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公开(公告)号:US20030104466A1
公开(公告)日:2003-06-05
申请号:US10314092
申请日:2002-12-05
IPC分类号: C12Q001/68 , C12P019/34 , C12M001/34
CPC分类号: C12Q1/6869 , B01F13/0059 , B01L3/0262 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502753 , B01L3/502784 , B01L7/52 , B01L2200/027 , B01L2200/0605 , B01L2200/0673 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0816 , B01L2300/0838 , B01L2300/0864 , B01L2300/0867 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , G01N2035/1053 , G01N2035/1058 , C12Q2565/629
摘要: Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and systems for performing a wide variety of fluid operations are provided. The devices and systems of are used in performing fluid operations which require a large number of iterative, successive or parallel fluid manipulations, in a microscale, or sealed and readily automated format.
摘要翻译: 提供了用于进行生物化学分析的综合系统,设备,软件和方法,包括DNA测序,基因组筛选,核酸和其他生物组分的纯化以及药物筛选。 提供了用于使用这些装置和系统进行各种流体操作的微流体装置,系统和方法。 这些装置和系统用于执行需要大量迭代,连续或平行流体操作的流体操作,其以微型或密封且容易自动的格式。
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公开(公告)号:US20030054425A1
公开(公告)日:2003-03-20
申请号:US10160289
申请日:2002-06-04
IPC分类号: G01N033/53 , G01N033/537 , G01N033/543
CPC分类号: G01N33/5091 , B01J19/0093 , B01L3/5025 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L9/527 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2200/0689 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0877 , B01L2300/0887 , B01L2400/0415 , G01N27/44791 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/5044 , G01N33/5064 , G01N33/5094 , G01N33/5304 , G01N33/57415 , G01N35/08 , Y10S366/02 , Y10S435/81 , Y10S435/97 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809
摘要: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
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公开(公告)号:US06100541A
公开(公告)日:2000-08-08
申请号:US30535
申请日:1998-02-24
申请人: Robert Nagle , Colin B. Kennedy
发明人: Robert Nagle , Colin B. Kennedy
CPC分类号: B01L3/5027 , B01L3/502715 , G01N21/0303 , B01L2300/0654 , B01L2300/0816 , B01L2300/0887 , B01L2400/0415 , B01L2400/0487
摘要: The present invention provides microfluidic devices and systems that utilize optical detection systems, and where one or more light altering optical elements are integrated into the body structure of the microfluidic device. The resulting devices perform at least a portion of the optical manipulations used in the optical detection method employed.
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公开(公告)号:US6086825A
公开(公告)日:2000-07-11
申请号:US274811
申请日:1999-03-23
CPC分类号: G01N27/44791 , B01J19/0093 , B01L3/0244 , B01L3/0262 , B01L3/5025 , B01L3/50255 , B01L3/5027 , B01L3/502715 , B01L3/50273 , G01N27/44743 , B01J2219/00783 , B01J2219/00826 , B01J2219/00831 , B01J2219/00833 , B01J2219/00853 , B01J2219/00891 , B01J2219/00909 , B01L2200/027 , B01L2300/0681 , B01L2300/0816 , B01L2300/0819 , B01L2300/0864 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0688 , B01L3/502723 , B01L3/502753 , G01N2035/00237 , G01N2035/1037 , Y10T436/25125 , Y10T436/25375 , Y10T436/2575
摘要: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.
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公开(公告)号:US6080295A
公开(公告)日:2000-06-27
申请号:US45339
申请日:1998-03-20
申请人: J. Wallace Parce , Michael R. Knapp
发明人: J. Wallace Parce , Michael R. Knapp
IPC分类号: G01N31/20 , B01F13/00 , B01J19/00 , B01L3/00 , B01L3/02 , F04B17/00 , F04B19/00 , G01N27/26 , G01N27/447 , G01N37/00 , H02N11/00
CPC分类号: B01F13/0071 , B01J19/0093 , B01L3/502715 , B01L3/50273 , B01L3/502784 , F04B17/00 , F04B19/006 , G01N27/44704 , G01N27/44743 , G01N27/44791 , H02N11/006 , B01F13/0084 , B01F13/0086 , B01J2219/00891 , B01L2200/027 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L3/021 , Y10S366/02 , Y10T436/113332 , Y10T436/2575
摘要: The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.
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