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    Method and apparatus for processing tissue samples
    4.
    发明授权
    Method and apparatus for processing tissue samples 有权

    公开(公告)号：US11231347B2

    公开(公告)日：2022-01-25

    申请号：US16301249

    申请日：2017-11-29

    申请人： S2 Genomics, Inc. ,  Stevan Jovanovich ,  Frank Zaugg ,  Kaiwan Chear ,  Roger McIntosh ,  Nathan Pereira

    发明人： Stevan Jovanovich ,  Frank Zaugg ,  Kaiwan Chear ,  Roger McIntosh ,  Nathan Pereira

    IPC分类号： C12M1/00 ,  G01N1/31 ,  C12Q1/6851 ,  C12N1/06 ,  C12N15/10 ,  G01N1/28

    摘要： A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.