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    Sequence Determination By Use Of Opposing Forces
    1.
    发明申请
    Sequence Determination By Use Of Opposing Forces 审中-公开
    使用反对力的序列确定

    公开(公告)号：US20110059864A1

    公开(公告)日：2011-03-10

    申请号：US12877103

    申请日：2010-09-07

    申请人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    发明人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    IPC分类号： C40B30/10 ,  C12M1/34

    CPC分类号： C12Q1/6872 ,  C12Q1/6874

    摘要： The present teachings relate to systems, methods, and the like, for analyzing biological polymers, by use of opposing forces. Among other things, the present teachings can be used to determine sequence information, such as in genetic sequencing and genotyping applications. Various embodiments are described for efficient, high throughput sequencing of nucleic-acid molecules, such as DNA. Various embodiments are described wherein nucleic-acid sequence information is determined without the need or use of extrinsic labels. As well various embodiments of methods, systems, and the like, are described, which can provide long and accurate read lengths for low-cost nucleic acid sequencing.

    摘要翻译： 本教导涉及通过使用相反的力分析生物聚合物的系统，方法等。 除其他之外，本教导可用于确定序列信息，例如在遗传测序和基因分型应用中。 描述了用于核酸分子（例如DNA）的有效，高通量测序的各种实施方案。 描述了其中确定核酸序列信息而不需要或使用外在标记的各种实施方案。 还描述了方法，系统等的各种实施方案，其可以为低成本核酸测序提供长且准确的读取长度。

    METHODS FOR SANGER SEQUENCING USING PARTICLE ASSOCIATED CLONAL AMPLICONS AND HIGHLY PARALLEL ELECTROPHORETIC SIZE-BASED SEPARATION
    2.
    发明申请
    METHODS FOR SANGER SEQUENCING USING PARTICLE ASSOCIATED CLONAL AMPLICONS AND HIGHLY PARALLEL ELECTROPHORETIC SIZE-BASED SEPARATION 审中-公开
    使用颗粒相关的克隆AMPLICONS和高度并行电泳大小分离的方法

    公开(公告)号：US20100112588A1

    公开(公告)日：2010-05-06

    申请号：US12611528

    申请日：2009-11-03

    申请人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    发明人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2563/149 ,  C12Q2535/122 ,  C12Q2535/101

    摘要： Methods for highly parallel Sanger sequencing are discussed. In particular, provided herein are methods using particles to clonally amplify templates and to introduce the amplified nucleic acids into many parallel channels with a single template per channel. Once in the channels, the nucleic acids are separated by size using electrophoresis to produce long read length sequencing information. Methods involving optical detection of the size-separated nucleic acids and analysis of the resulting electropherograms to yield the sequences are disclosed.

    摘要翻译： 讨论了高度并行Sanger测序的方法。 特别地，本文提供了使用颗粒克隆放大模板并将扩增的核酸引入具有每个通道的单个模板的多个并行通道的方法。 一旦在通道中，核酸通过电泳被大小分离以产生长的读取长度测序信息。 公开了涉及尺寸分离的核酸的光学检测和所得的电泳图的分析以产生序列的方法。

    METHODS AND APPARATUS FOR BINDING ASSAYS
    3.
    发明申请
    METHODS AND APPARATUS FOR BINDING ASSAYS 审中-公开
    用于绑定测试的方法和装置

    公开(公告)号：US20120220486A1

    公开(公告)日：2012-08-30

    申请号：US13505455

    申请日：2010-10-31

    申请人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    发明人： Javier Farinas ,  Andrea Chow ,  John Wallace Parce

    IPC分类号： G01N33/53 ,  C40B30/04

    CPC分类号： C12Q1/6825 ,  C12Q1/6834 ,  G01N33/54346 ,  C12Q2565/107 ,  C12Q2523/303 ,  C12Q2565/607

    摘要： The present teachings relate to methods, systems, and apparatus for low cost label-free assay detection. The present teachings, in a variety of embodiments, employ opposing forces to detect signals which depend on the number of charges on and/or the size of a particle. The particle, which can be subjected to opposing forces, can have specific capture probes at its surface. As analytes of interest are captured by the particle, the number of charges on the particle surface and/or the size of the particle is changed. A particle parameter or kinematic property such as the position, velocity, acceleration or force of/on the particle can be measured, and results obtained relating, for example, to the present, absence, quantity, and such, of one or more analytes of interest. Various embodiments are described for efficient, high throughput assays of samples potentially including one or more analytes of interest, such as bioanalytes. As well, various embodiments are described wherein binding assays can be carried out without the need or use of extrinsic labels. A number of embodiments provide, for example, methods, systems, and apparatus for detecting analytes (such as nucleic acids, proteins, cells and other entities, particulates, and the like) in one or more samples. Also described are: detection of a single copy of a target biomolecule, such as DNA, captured onto a trapped (e.g., tethered) bead; protocols for fabricating encoded bead arrays for multiplex assays; and methods, systems and apparatus for efficient and specific capture of pathogen biomolecular markers onto bead-bound capture probes, as well as detection and measurement of such capture events.

    摘要翻译： 本教导涉及用于低成本无标签测定检测的方法，系统和装置。 在各种实施例中，本教导使用相反的力来检测取决于粒子的数量和/或粒子尺寸的信号。 可以受到相反力的颗粒在其表面可以具有特定的捕获探针。 当感兴趣的分析物被颗粒俘获时，粒子表面上的电荷数和/或粒子的大小被改变。 可以测量颗粒参数或运动学特性，例如颗粒上的/位置，速度，加速度或力，或者与例如当前，不存在，数量等相关的一种或多种分析物 利益。 描述了针对可能包括一种或多种感兴趣的分析物（例如生物分析物）的样品的有效，高通量测定的各种实施方案。 同样，描述了各种实施方案，其中可以在不需要或使用外在标记的情况下进行结合测定。 许多实施例提供了例如在一个或多个样品中检测分析物（例如核酸，蛋白质，细胞和其他实体，颗粒等）的方法，系统和装置。 还描述了：捕获到捕获的（例如，系留的）珠粒上的目标生物分子（例如DNA）的单拷贝的检测; 用于制备用于多重测定的编码珠阵列的方案; 以及用于将有效和特异性捕获病原体生物分子标记物转移到珠束捕获探针上的方法，系统和装置，以及这些捕获事件的检测和测量。

    Micropump
    6.
    发明授权
    Micropump 失效

    公开(公告)号：US06568910B1

    公开(公告)日：2003-05-27

    申请号：US10114430

    申请日：2002-04-02

    申请人： John Wallace Parce

    发明人： John Wallace Parce

    IPC分类号： F04B3702

    CPC分类号： B01L3/50273 ,  B01L3/502707 ,  B01L2300/0816 ,  B01L2400/0418 ,  B01L2400/0487 ,  F04B19/006 ,  H02N11/006

    摘要： The present invention generally provides a micropump that utilizes electroosmotic pumping of fluid in one channel or region to generate a pressure based flow of material in a connected channel, where the connected channel has substantially no electroosmotic flow generated. Such pumps have a variety of applications, and are particularly useful in those situations where the application for which the pump is to be used prohibits the application of electric fields to the channel in which fluid flow is desired, or where pressure based flow is particularly desirable.