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公开(公告)号：US20070155016A1
公开(公告)日：2007-07-05
申请号：US11466104
申请日：2006-08-21
申请人： Luke Lee , Jeonggi Seo , Cristian Ionescu-Zanetti , Michelle Khine , Adrian Lau
发明人： Luke Lee , Jeonggi Seo , Cristian Ionescu-Zanetti , Michelle Khine , Adrian Lau
CPC分类号： G01N33/48728 , B01L3/502707 , B01L3/502761 , B01L2200/0668 , B01L2400/0487 , C12M23/16 , C12M35/02 , C12N15/87
摘要： Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.
摘要翻译： 方法和系统在使用横向细胞捕获的微流体系统和装置中提供改进的细胞处理以及允许选择性低电压电穿孔和电融合的制造方法。
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2.发明申请公开(公告)号：US20120135887A1
公开(公告)日：2012-05-31
申请号：US13291018
申请日：2011-11-07
CPC分类号： G01N33/48728 , B01L3/502707 , B01L3/502761 , B01L2200/0668 , B01L2400/0487 , C12M23/16 , C12M35/02 , C12N15/87
摘要： Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.
摘要翻译： 方法和系统在使用横向细胞捕获的微流体系统和装置中提供改进的细胞处理以及允许选择性低电压电穿孔和电融合的制造方法。
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公开(公告)号：US08058056B2
公开(公告)日：2011-11-15
申请号：US11466104
申请日：2006-08-21
IPC分类号： C12M3/00
CPC分类号： G01N33/48728 , B01L3/502707 , B01L3/502761 , B01L2200/0668 , B01L2400/0487 , C12M23/16 , C12M35/02 , C12N15/87
摘要： Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.
摘要翻译： 方法和系统在使用横向细胞捕获的微流体系统和装置中提供改进的细胞处理以及允许选择性低电压电穿孔和电融合的制造方法。
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公开(公告)号：US20110045582A1
公开(公告)日：2011-02-24
申请号：US10598830
申请日：2005-03-14
申请人： Luke P. Lee , Jeonggi Seo , Cristian Ionescu-Zanetti , Michelle Khine , Poorya Sabounchi , Robin Shaw
发明人： Luke P. Lee , Jeonggi Seo , Cristian Ionescu-Zanetti , Michelle Khine , Poorya Sabounchi , Robin Shaw
CPC分类号： G01N33/48728 , B01L3/502707 , B01L3/502761 , B01L2200/0668 , B01L2200/12 , B01L2200/16 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2400/049 , B01L2400/0655 , B29C33/3842 , B29C45/372
摘要： Method and systems that provide improved cell handling and assays in microfluidic systems and devices particularly using lateral cell trapping and methods of fabrication of the same.
摘要翻译： 在微流体系统和装置中提供改进的细胞处理和测定的方法和系统，特别是使用横向细胞捕获及其制备方法。
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5.发明授权Methods and apparatus for the manipulation of particle suspensions and testing thereof 有权用于操作颗粒悬浮液的方法和设备及其测试公开(公告)号：US08293524B2
公开(公告)日：2012-10-23
申请号：US11690831
申请日：2007-03-25
CPC分类号： G01N15/1404 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502761 , B01L3/565 , B01L2200/025 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2200/0689 , B01L2300/041 , B01L2300/046 , B01L2300/0627 , B01L2300/0645 , B01L2300/0654 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/163 , B01L2400/0487 , B01L2400/086 , C12M21/06 , C12M23/16 , C12M35/04 , C12M41/36 , G01N15/1459 , G01N15/1463 , G01N15/1475 , G01N15/1484 , G01N2015/1006 , G01N2015/1075 , G01N2015/1409 , G01N2015/1495 , G01N2015/1497
摘要： Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.
摘要翻译： 提供了用于分析微流体装置中的各个颗粒的装置和方法。 这些方法涉及在悬浮液中固定一系列颗粒并应用实验化合物。 这样的方法还可以包括电生理学研究，包括在同一微流体装置中的膜片钳记录，电穿孔或两者。 提供的装置包括耦合到多孔结构的微流体装置和用于控制微通道装置内的介质流动的界面。
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6.发明申请Methods and Apparatus for the Manipulation of Particle Suspensions and Testing Thereof 有权用于操作颗粒悬浮液及其测试的方法和装置公开(公告)号：US20070243523A1
公开(公告)日：2007-10-18
申请号：US11690831
申请日：2007-03-25
CPC分类号： G01N15/1404 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502761 , B01L3/565 , B01L2200/025 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2200/0689 , B01L2300/041 , B01L2300/046 , B01L2300/0627 , B01L2300/0645 , B01L2300/0654 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/163 , B01L2400/0487 , B01L2400/086 , C12M21/06 , C12M23/16 , C12M35/04 , C12M41/36 , G01N15/1459 , G01N15/1463 , G01N15/1475 , G01N15/1484 , G01N2015/1006 , G01N2015/1075 , G01N2015/1409 , G01N2015/1495 , G01N2015/1497
摘要： Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.
摘要翻译： 提供了用于分析微流体装置中的各个颗粒的装置和方法。 这些方法涉及在悬浮液中固定一系列颗粒并应用实验化合物。 这样的方法还可以包括电生理学研究，包括在同一微流体装置中的膜片钳记录，电穿孔或两者。 提供的装置包括耦合到多孔结构的微流体装置和用于控制微通道装置内的介质流动的界面。
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7.发明申请METHODS AND APPARATUS FOR THE MANIPULATION OF PARTICLE SUSPENSIONS AND TESTING THEREOF 审中-公开用于操作颗粒悬浮液及其测试的方法和装置公开(公告)号：US20120264134A1
公开(公告)日：2012-10-18
申请号：US13454849
申请日：2012-04-24
CPC分类号： G01N15/1404 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/502761 , B01L3/565 , B01L2200/025 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2200/0689 , B01L2300/041 , B01L2300/046 , B01L2300/0627 , B01L2300/0645 , B01L2300/0654 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/163 , B01L2400/0487 , B01L2400/086 , C12M21/06 , C12M23/16 , C12M35/04 , C12M41/36 , G01N15/1459 , G01N15/1463 , G01N15/1475 , G01N15/1484 , G01N2015/1006 , G01N2015/1075 , G01N2015/1409 , G01N2015/1495 , G01N2015/1497
摘要： Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.
摘要翻译： 提供了用于分析微流体装置中的各个颗粒的装置和方法。 这些方法涉及在悬浮液中固定一系列颗粒并应用实验化合物。 这样的方法还可以包括电生理学研究，包括在同一微流体装置中的膜片钳记录，电穿孔或两者。 提供的装置包括耦合到多孔结构的微流体装置和用于控制微通道装置内的介质流动的界面。
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8.发明申请PROCESSES FOR RAPID MICROFABRICATION USING THERMOPLASTICS AND DEVICES THEREOF 有权使用热塑性物质进行快速微生物处理的方法及其设备公开(公告)号：US20110122406A1
公开(公告)日：2011-05-26
申请号：US12742598
申请日：2008-11-12
申请人： Michelle Khine , Chi-shuo Chen , Anthoy Grimes , David Nate Breslauer , Luke Lee , Michael Dunlap , Ajay Gopinathan , Sayantani Ghosh
发明人： Michelle Khine , Chi-shuo Chen , Anthoy Grimes , David Nate Breslauer , Luke Lee , Michael Dunlap , Ajay Gopinathan , Sayantani Ghosh
CPC分类号： B01L3/502707 , B01F5/0057 , B01F5/0646 , B01F5/0647 , B01F13/0059 , B01F2215/0037 , B01L2200/12 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B81B1/002 , B81B2201/051 , B81B2201/058 , B81B2203/0338 , B81C1/00071 , B81C2201/0152 , B82Y40/00 , G03F7/0002 , H01L21/02269 , H01L21/02271
摘要： A method is provided to prepare one or more microfluidic channels on a receptive material by applying an image-forming material to a heat sensitive thermoplastic receptive material in a designed pattern and heating the material under conditions that reduce the size of the thermoplastic receptive material by at least about 60%. In an alternative aspect, the microfluidic channels on receptive material are prepared by etching a designed pattern into a heat sensitive thermoplastic material support and then heating the material under conditions that reduce the size of the thermoplastic receptive material by at least about 60%.
摘要翻译： 提供了一种通过将图像形成材料以设计图案施加到热敏性热塑性接受材料上并在加热材料的条件下，通过在下述条件下加热该材料的方法来制备接受材料上的一个或多个微流体通道： 至少约60％。 在另一方面，接收材料上的微流体通道是通过将设计的图案蚀刻成热敏热塑性材料载体，然后在将热塑性接受材料的尺寸减小至少约60％的条件下加热材料来制备的。
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9.发明授权Processes for rapid microfabrication using thermoplastics and devices thereof 有权使用热塑性塑料快速微细加工的方法及其装置公开(公告)号：US09522820B2
公开(公告)日：2016-12-20
申请号：US12742598
申请日：2008-11-12
申请人： Michelle Khine , Chi-shuo Chen , Anthony Grimes , David Nate Breslauer , Luke Lee , Michael Dunlap , Ajay Gopinathan , Sayantani Ghosh
发明人： Michelle Khine , Chi-shuo Chen , Anthony Grimes , David Nate Breslauer , Luke Lee , Michael Dunlap , Ajay Gopinathan , Sayantani Ghosh
CPC分类号： B01L3/502707 , B01F5/0057 , B01F5/0646 , B01F5/0647 , B01F13/0059 , B01F2215/0037 , B01L2200/12 , B01L2300/0816 , B01L2300/0867 , B01L2300/0887 , B81B1/002 , B81B2201/051 , B81B2201/058 , B81B2203/0338 , B81C1/00071 , B81C2201/0152 , B82Y40/00 , G03F7/0002 , H01L21/02269 , H01L21/02271
摘要： A method is provided to prepare one or more microfluidic channels on a receptive material by applying an image-forming material to a heat sensitive thermoplastic receptive material in a designed pattern and heating the material under conditions that reduce the size of the thermoplastic receptive material by at least about 60%. In an alternative aspect, the microfluidic channels on receptive material are prepared by etching a designed pattern into a heat sensitive thermoplastic material support and then heating the material under conditions that reduce the size of the thermoplastic receptive material by at least about 60%.
摘要翻译： 提供了一种通过将图像形成材料以设计图案施加到热敏性热塑性接受材料上并在加热材料的条件下，通过在下述条件下加热该材料的方法来制备接受材料上的一个或多个微流体通道： 至少约60％。 在另一方面，接收材料上的微流体通道是通过将设计的图案蚀刻成热敏热塑性材料载体，然后在将热塑性接受材料的尺寸减小至少约60％的条件下加热材料来制备的。
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公开(公告)号：US20120129209A1
公开(公告)日：2012-05-24
申请号：US13257019
申请日：2010-03-18
申请人： Michelle Khine , Jesus Isaac Luna
发明人： Michelle Khine , Jesus Isaac Luna
CPC分类号： C12N5/0068 , C12N5/0657 , C12N2535/10 , G01N33/502
摘要： A method is provided for preparing an aligned cell population comprising the culturing one or more cells on a surface having a texture, which texture has an average height of from about 100 nanometers to about 5 micrometers, thereby forming an aligned cell population on the textured surface. Also provided is a method to prepare the surface which method comprises the steps of: a) depositing a metal onto an unstressed or pre-stressed thermoplastic material; b) reducing the surface area of the receptive material by at least about 60%; and c) preparing the surface via lithography.
摘要翻译： 提供了一种用于制备排列的细胞群的方法，其包括在具有纹理的表面上培养一个或多个细胞，该纹理的平均高度为约100纳米至约5微米，从而在纹理表面上形成排列的细胞群 。 还提供了一种制备表面的方法，该方法包括以下步骤：a）将金属沉积到未受应力或预应力的热塑性材料上; b）将接收材料的表面积减少至少约60％; 和c）通过光刻制备表面。
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