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    APPARATUS AND METHOD FOR CULTURING STEM CELLS
    3.
    发明申请
    APPARATUS AND METHOD FOR CULTURING STEM CELLS 审中-公开
    培养干细胞的装置和方法

    公开(公告)号：US20120064627A1

    公开(公告)日：2012-03-15

    申请号：US13145546

    申请日：2010-01-25

    申请人： Michelle Khine ,  Chi-Shuo Chen ,  Wei-chun Chin

    发明人： Michelle Khine ,  Chi-Shuo Chen ,  Wei-chun Chin

    IPC分类号： C12N5/02 ,  B29C59/02 ,  C12M1/00

    CPC分类号： C12M23/20 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/0893 ,  B82Y10/00 ,  B82Y40/00 ,  C12M23/12 ,  G03F7/0002

    摘要： A method is provided to prepare a plurality of microwells suitable for the formation of embryoid bodies from embryonic stem cells. The method requires applying an image-forming material to a heat sensitive thermoplastic material in a designed pattern and heating the material under conditions that reduce the size of the receptive material by at least about 60% to create a mold. A polymer such as PDMS is then applied to the mold and removed to form the microwells. In an alternative aspect, the plurality of microwells on receptive material are prepared by etching a microwell designed pattern into a heat sensitive thermoplastic material support and then heating the material under conditions that reduce the size of the receptive material by at least about 60%.

    摘要翻译： 提供了一种制备适合于从胚胎干细胞形成胚状体的多个微孔的方法。 该方法需要以设计图案将成像材料施加到热敏热塑性材料上，并在将接受材料的尺寸减小至少约60％的条件下加热该材料以产生模具。 然后将诸如PDMS的聚合物施加到模具上并除去以形成微孔。 在另一方面，接收材料上的多个微孔通过将微孔设计图案蚀刻成热敏热塑性材料载体然后在将接受材料的尺寸减小至少约60％的条件下进行加热来制备。

    ALIGNING CELLS ON WRINKLED SURFACE
    7.
    发明申请
    ALIGNING CELLS ON WRINKLED SURFACE 审中-公开
    皱纹表面上的标记细胞

    公开(公告)号：US20120129209A1

    公开(公告)日：2012-05-24

    申请号：US13257019

    申请日：2010-03-18

    申请人： Michelle Khine ,  Jesus Isaac Luna

    发明人： Michelle Khine ,  Jesus Isaac Luna

    IPC分类号： C12N5/071 ,  C12N5/079 ,  C12Q1/02 ,  C12N5/077

    CPC分类号： C12N5/0068 ,  C12N5/0657 ,  C12N2535/10 ,  G01N33/502

    摘要： A method is provided for preparing an aligned cell population comprising the culturing one or more cells on a surface having a texture, which texture has an average height of from about 100 nanometers to about 5 micrometers, thereby forming an aligned cell population on the textured surface. Also provided is a method to prepare the surface which method comprises the steps of: a) depositing a metal onto an unstressed or pre-stressed thermoplastic material; b) reducing the surface area of the receptive material by at least about 60%; and c) preparing the surface via lithography.

    摘要翻译： 提供了一种用于制备排列的细胞群的方法，其包括在具有纹理的表面上培养一个或多个细胞，该纹理的平均高度为约100纳米至约5微米，从而在纹理表面上形成排列的细胞群 。 还提供了一种制备表面的方法，该方法包括以下步骤：a）将金属沉积到未受应力或预应力的热塑性材料上; b）将接收材料的表面积减少至少约60％; 和c）通过光刻制备表面。

    PHOTOLITHOGRAPHY ON SHRINK FILM
    9.
    发明申请
    PHOTOLITHOGRAPHY ON SHRINK FILM 有权
    闪光膜的光刻技术

    公开(公告)号：US20130101795A1

    公开(公告)日：2013-04-25

    申请号：US13481653

    申请日：2012-05-25

    申请人： Michelle Khine ,  Shreshta Jayadev

    发明人： Michelle Khine ,  Shreshta Jayadev

    IPC分类号： G03F7/20

    CPC分类号： G03F7/20 ,  G03F7/0005 ,  Y10T428/24479

    摘要： Provided are methods of preparing a plurality of extrusions, comprising (a) shrinking a transparent first thermoplastic material comprising a plurality of non-transparent marks, (b) lithographically transferring the pattern of the shrunk non-transparent marks to a layer of photoresist deposited on a second thermoplastic material, and (c) shrinking the second thermoplastic material, thereby generating a plurality of extrusions on the shrunk second thermoplastic material.

    摘要翻译： 提供了制备多个挤出物的方法，其包括（a）收缩包含多个不透明标记的透明第一热塑性材料，（b）将收缩的非透明标记的图案光刻转印到沉积在 第二热塑性材料，和（c）收缩第二热塑性材料，从而在收缩的第二热塑性材料上产生多个挤出物。