公开(公告)号：US07824618B2

公开(公告)日：2010-11-02

申请号：US10913422

申请日：2004-08-09

申请人： Yong Shin Kim ,  Yun Tae Kim ,  Hae Sik Yang ,  Young Jun Kim ,  Seung Chul Ha ,  Yoon Seok Yang

发明人： Yong Shin Kim ,  Yun Tae Kim ,  Hae Sik Yang ,  Young Jun Kim ,  Seung Chul Ha ,  Yoon Seok Yang

IPC分类号： G01N27/00

CPC分类号： G01N27/126

摘要： Provided is a sensor structure comprising a heater and a temperature sensor at the center of a membrane having a well structure, allowing a temperature to be rapidly controlled with low power, and the object is analyzed using a conductivity change measured at two or more substrate temperatures with a pair of detecting electrode and a detecting layer implemented on the heater, wherein the sensing layer can include a conductive particle and a non-conductive organic composite.

摘要翻译： 提供了一种传感器结构，其包括加热器和在具有井结构的膜的中心处的温度传感器，允许以低功率快速控制温度，并且使用在两个或更多个基板温度下测量的电导率变化来分析物体 具有一对检测电极和在加热器上实施的检测层，其中感测层可以包括导电颗粒和非导电有机复合材料。

公开(公告)号：US07871572B2

公开(公告)日：2011-01-18

申请号：US10944939

申请日：2004-09-21

申请人： Young Jun Kim ,  Yong Shin Kim ,  Yoon Seok Yang ,  Seung Chul Ha ,  Hae Sik Yang ,  Yun Tae Kim ,  Dae Sik Lee ,  Young Sik Hong

发明人： Young Jun Kim ,  Yong Shin Kim ,  Yoon Seok Yang ,  Seung Chul Ha ,  Hae Sik Yang ,  Yun Tae Kim ,  Dae Sik Lee ,  Young Sik Hong

IPC分类号： G01N27/04 ,  G01N27/22 ,  G01N33/00 ,  G01N33/553

CPC分类号： B82Y30/00 ,  B01J2219/005 ,  B01J2219/00648 ,  B01J2219/00702 ,  B01J2219/0072 ,  B82Y15/00 ,  G01N27/127 ,  G01N33/4972 ,  Y10T436/203332 ,  Y10T436/204165 ,  Y10T436/21 ,  Y10T436/212

摘要： Provided are mixed ligand metal nanoparticle chemical sensors in which metal nanoparticles are encapsulated by at least two kinds of different molecule ligands having a relatively low conductivity and various composition ratios, and a chemical sensor array in which a film of the metal nanoparticle sensor is formed on the substrate. The metal nanoparticle sensor using the mixed ligand improves sensitivity and reaction speed with respect to an analyte, and selectivity with respect to various analytes, and a kind and a composition of a ligand of the mixed ligand constituting the metal nanoparticle sensor are adjusted to allow the high sensitivity nanoparticle sensor to be applied to the sensor array technology, thereby enabling a design of sensor properties as well as systematic access to a configuration of the sensor array the most efficient for the analytes.

摘要翻译： 提供了混合的配体金属纳米颗粒化学传感器，其中金属纳米颗粒被具有相对低的导电性和各种组成比的至少两种不同的分子配体包封，以及化学传感器阵列，其中金属纳米颗粒传感器的膜形成在 底物。 使用混合配体的金属纳米颗粒传感器改善了对分析物的敏感性和反应速度，并且相对于各种分析物的选择性以及构成金属纳米颗粒传感器的混合配体的配体的种类和组成被调整，以允许 高灵敏度纳米粒子传感器应用于传感器阵列技术，从而实现传感器特性的设计，以及对分析物最有效的系统访问传感器阵列的配置。

公开(公告)号：US07652370B2

公开(公告)日：2010-01-26

申请号：US10899177

申请日：2004-07-27

申请人： Dae Sik Lee ,  Hae Sik Yang ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

发明人： Dae Sik Lee ,  Hae Sik Yang ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

IPC分类号： H01L29/06

CPC分类号： B01J19/0093 ,  B01J2219/00495 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00783 ,  B01J2219/00804 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00858 ,  B01J2219/00873 ,  B01J2219/00961 ,  B01L3/502707 ,  B01L3/50851 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/1827 ,  B33Y80/00 ,  C40B60/14 ,  G01N27/128

摘要： Provided are a plastic microfabricated structure, and a microfabricated thermal device, a microfabricated reactor, a microfabricated reactor array and a micro array using the same, which may be applied to a bio chip, and the present invention may fabricate the plastic microfabricated structure for providing a heating region by means of insulating plastic, which has a thin thickness, flatness enough to allow a photolithography process to be performed, thermal isolation in its some or total area, and a small thermal mass, and on top of the heating region of the plastic microfabricated structure, a heater, a temperature sensor for sensing a temperature, an electrode, and an electrode pad are formed to thereby fabricate the microfabricated heating device, whereby element may be readily fabricated at a low cost, and the heating region is formed of a plastic thin layer, so that uniform temperature control is possible even with a low power, and various samples may be thermally treated at a fast speed to obtain their reaction and analysis.

摘要翻译： 本发明提供一种塑料微制造结构，微制造热装置，微制造反应器，微制造反应器阵列和使用其的微阵列，其可以应用于生物芯片，并且本发明可以制造塑料微制造结构以提供 通过绝缘塑料的加热区域，其具有薄的厚度，足够的平坦度，以允许执行光刻工艺，其一些或总面积的热隔离和小的热质量，以及在 形成塑料微加工结构，加热器，用于感测温度的温度传感器，电极和电极焊盘，从而制造微制造加热装置，从而可以以低成本容易地制造元件，并且加热区域由 塑料薄层，使得即使在低功率下也能进行均匀的温度控制，并且各种样品可以在fa处进行热处理 速度获得反应和分析。

公开(公告)号：US07183056B2

公开(公告)日：2007-02-27

申请号：US10697295

申请日：2003-10-31

申请人： Se Ho Park ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Beom Shin ,  Kyu Won Kim ,  Tae Hwan Yoon ,  Sung Jin Kim ,  Yun Tae Kim

发明人： Se Ho Park ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Beom Shin ,  Kyu Won Kim ,  Tae Hwan Yoon ,  Sung Jin Kim ,  Yun Tae Kim

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q2537/143 ,  C12Q2527/113 ,  C12Q2527/101

摘要： The present invention is directed to an improved multiplex PCR method for obtaining at least two PCR products from one PCR solution. In the multiplex PCR method for having at least two DNA amplified products from a sample positioned in a PCR equipment, the object of the present invention is to provide a novel multiplex PCR method characterized in that a primer annealing temperature and an extension time be changed per cycle with constant periods. When a multiplex PCR is performed in accordance with the present invention, limitations in determining PCR conditions due to a various sizes of PCR products or dimers caused by primers can be eliminated, and time and efforts required for determining the PCR conditions to various samples can also be reduced. Further, not only refined DNAs like cDNA, genomic DNA, vector, etc, but blood can be directly used as a multiplex PCR sample, and PCR amplification reaction can be performed even with a sample having the smallest amounts.

摘要翻译： 本发明涉及一种用于从一种PCR溶液获得至少两种PCR产物的改进的多重PCR方法。 本发明的目的是提供一种新的多重PCR方法，其特征在于，引物退火温度和延长时间根据 周期不变的周期。 当根据本发明进行多重PCR时，可以消除由于由引物引起的各种大小的PCR产物或二聚体导致的PCR条件的限制的限制，并且确定各种样品的PCR条件所需的时间和努力也可以 减少 此外，不仅可以将cDNA，基因组DNA，载体等精制DNA与血液直接用作多重PCR样品，即使使用量少的样品也能进行PCR扩增反应。

公开(公告)号：US07362387B2

公开(公告)日：2008-04-22

申请号：US11299111

申请日：2005-12-08

申请人： Moon Youn Jung ,  Hae Sik Yang ,  Chi Hoon Jun ,  Yun Tae Kim ,  Young Shin Kim ,  Dong Ho Shin

发明人： Moon Youn Jung ,  Hae Sik Yang ,  Chi Hoon Jun ,  Yun Tae Kim ,  Young Shin Kim ,  Dong Ho Shin

IPC分类号： G02F1/13

CPC分类号： B01J19/0046 ,  B01J2219/00434 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y30/00 ,  C40B40/06 ,  C40B60/14 ,  G02F1/133528 ,  G02F1/13454 ,  G02F1/13725 ,  G02F1/216 ,  G02F2203/12 ,  G03F7/70291 ,  G03F7/7035 ,  H01L27/146

摘要： A programmable mask used in a photolithography process for fabricating a biomolecule array and a method for fabricating a biomolecule array using the same are disclosed. Particularly, a TFT-LCD type programmable mask for selectively transmitting incident light in accordance with an electrical signal applied thereto and a method for fabricating a biomolecule array using the same are provided. The ultraviolet light is selectively illuminated to a sample substrate so that the biomolecule array having high density can be fabricated.

摘要翻译： 公开了用于制造生物分子阵列的光刻工艺中使用的可编程掩模和使用其的生物分子阵列的制造方法。 特别地，提供了根据施加到其上的电信号选择性地透射入射光的TFT-LCD型可编程掩模和使用其的制造生物分子阵列的方法。 紫外光被选择性地照射到样品基底上，使得可以制造具有高密度的生物分子阵列。

公开(公告)号：US07238324B2

公开(公告)日：2007-07-03

申请号：US10615441

申请日：2003-07-07

申请人： Jong Soo Ko ,  Hyun Chul Yoon ,  Hae Sik Yang ,  Dae-Sik Lee ,  Kwang Hyo Chung ,  Hyeon Bong Pyo ,  Sung Jin Kim ,  Yun Tae Kim

发明人： Jong Soo Ko ,  Hyun Chul Yoon ,  Hae Sik Yang ,  Dae-Sik Lee ,  Kwang Hyo Chung ,  Hyeon Bong Pyo ,  Sung Jin Kim ,  Yun Tae Kim

IPC分类号： B01L3/02

CPC分类号： B01L3/502707 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L2400/088 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T436/25

摘要： A microfluidic device for controlling the flow of a micro amount of fluid is provided. The microfluidic device is manufactured by binding a sensing substrate including a sensing electrode, an electrode interconnect, and a electrode pad, with a channel substrate including at least two fluid inlet ports, a chamber, and a channel, wherein a first fluid injected via one of the fluid inlet ports flows by natural capillary force, and a second fluid injected via another fluid inlet port is forced to flow by an external pump. The microfluidic device controls fluid flow and flow stoppage by a combination of natural capillary flow and an externally applied pressure as a result of the action of a pump.

摘要翻译： 提供了用于控制微量流体流动的微流体装置。 微流体装置通过将包括感测电极，电极互连和电极焊盘的感测衬底与包括至少两个流体入口端口，腔室和通道的通道衬底结合来制造，其中通过一个注入的第一流体 的流体入口端口通过天然毛细管力流动，并且通过另一流体入口喷射的第二流体被迫使外部泵流动。 由于泵的作用，微流体装置通过天然毛细管流动和外部施加的压力的组合来控制流体流动和流动停止。

公开(公告)号：US07682571B2

公开(公告)日：2010-03-23

申请号：US10899143

申请日：2004-07-27

申请人： Sung Jin Kim ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Kyu Won Kim ,  Se Ho Park ,  Yun Tae Kim

发明人： Sung Jin Kim ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Kyu Won Kim ,  Se Ho Park ,  Yun Tae Kim

IPC分类号： B01L3/00

CPC分类号： B01L3/502707 ,  B01J2219/00286 ,  B01J2219/00389 ,  B01J2219/00495 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00869 ,  B01J2219/00873 ,  B01L7/00 ,  B01L2300/1827 ,  C40B60/14 ,  H05B3/26 ,  H05B2203/003 ,  H05B2203/013 ,  H05B2203/017

摘要： Provided is a micro-fluidic heating system, which comprises a micro-fluidic control element for providing a chamber, a flow path and a valve, and a main body for heating the inside of the chamber in contact with the micro-fluidic control element, wherein the micro-fluidic control element consists of an upper substrate for providing the chamber, the flow path and the valve, and a lower substrate as a thin film bonded to the upper substrate, and the main body consists of a membrane in which heating means and suction holes are formed, and support member for supporting the membrane, and the heating means is partially in contact with the lower substrate of the chamber to heat the inside of the chamber, so that thermal transfer efficiency becomes maximized and temperature of each chamber may be independently controlled in the case of configuration having chambers arranged in array.

摘要翻译： 提供了一种微流体加热系统，其包括用于提供腔室，流路和阀门的微流控制元件和用于加热与微流控制元件接触的腔室内部的主体， 其特征在于，所述微流体控制元件由用于提供所述室，所述流路和所述阀的上基板以及与所述上基板接合的薄膜的下基板构成，所述主体由膜构成，加热单元 并且形成吸入孔，并且用于支撑膜的支撑构件，并且加热装置部分地与室的下基板接触以加热室的内部，使得热传递效率变得最大，并且每个室的温度可以 在配置有排列成阵列的腔室的情况下，可独立控制。

公开(公告)号：US07445754B2

公开(公告)日：2008-11-04

申请号：US10671490

申请日：2003-09-29

申请人： Kwang Hyo Chung ,  Jong Soo Ko ,  Hyun Chul Yoon ,  Hae Sik Yang ,  Hyeon Bong Pyo ,  Sung Jin Kim ,  Yun Tae Kim

发明人： Kwang Hyo Chung ,  Jong Soo Ko ,  Hyun Chul Yoon ,  Hae Sik Yang ,  Hyeon Bong Pyo ,  Sung Jin Kim ,  Yun Tae Kim

IPC分类号： B01L3/02

CPC分类号： B01L3/5027 ,  B01J2219/00837 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2400/0688 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T137/2076 ,  Y10T436/2575

摘要： Disclosed is to a device for exchanging minimum amount of the fluid only by using surface tension of the fluid instead of external force like a pump. According to the device, an additional pump and power supply is not needed, thereby the device can be small sized and portable, and not only manufacturing cost decreases and yield increases but the device is hardly out of order.

摘要翻译： 公开了仅通过使用流体的表面张力而不是像泵这样的外力来交换最小量的流体的装置。 根据该装置，不需要附加的泵和电源，因此该装置可以小型化且便于携带，不仅制造成本降低并且产量增加，而且装置几乎不会出现故障。

公开(公告)号：US07412990B2

公开(公告)日：2008-08-19

申请号：US10898964

申请日：2004-07-27

申请人： Kwang Hyo Chung ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taek Lim ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

发明人： Kwang Hyo Chung ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Taek Lim ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

IPC分类号： F15C1/06

CPC分类号： B01F13/0086 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0621 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0622 ,  B01L2400/0688 ,  B33Y70/00 ,  B33Y80/00 ,  Y10T137/2082 ,  Y10T137/2224

摘要： Provided is a microfluidic control device and method for controlling the microfluid, and a fine amount of fluid is controlled even with natural fluid flow and solution injection, wherein a pressure barrier of a capillary is removed by a surface tension change resulted from the solution injection to thereby obtain transport, interflow, mixing, and time delay of the microfluid, and to detail this, solution is injected to meet the boundary surface of the stopped fluid when the fluid is stopped by the stop valve, so that a function of the stop valve is removed to obtain the transport, interflow, and mixing of the fluid, and the method for controlling the microfluid may be applied to the microfluidic control device for biochemical reaction, and it uses only the capillary force change resulted from solution injection to thereby have its structure simplified.

摘要翻译： 提供了一种用于控制微流体的微流控制装置和方法，并且即使使用天然流体流动和溶液注入也能控制微量的流体，其中毛细管的压力屏障由于溶液注入导致的表面张力变化而被去除 从而获得微流体的运输，流动，混合和时间延迟，并且为了详细说明，当流体被截止阀停止时，溶液被注入以满足停止的流体的边界表面，使得截止阀 被移除以获得流体的输送，流动和混合，并且用于控制微流体的方法可以应用于用于生物化学反应的微流控制装置，并且其仅使用由溶液注入引起的毛细管力变化，从而使其 结构简化。

公开(公告)号：US06896780B2

公开(公告)日：2005-05-24

申请号：US10020774

申请日：2001-12-11

申请人： Hae Sik Yang ,  Chi Hoon Jun ,  Chang Auck Choi ,  Youn Tae Kim

发明人： Hae Sik Yang ,  Chi Hoon Jun ,  Chang Auck Choi ,  Youn Tae Kim

IPC分类号： B81B7/04 ,  B01J19/00 ,  G01N27/403 ,  G01N27/28 ,  G01N27/327

CPC分类号： B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00853 ,  B01J2219/00873 ,  G01N27/403

摘要： The present invention relates to a microelectrode, a microelectrode array, and a method of manufacturing the microelectrode of which temperature can be controlled. The microelectrode comprises a sealed cavity formed in a silicon substrate for thermal isolation, a microheater formed on the sealed cavity, and an electrode heated indirectly by the microheater. According to the present invention, it is possible to manufacture with CMOS process the microelectrode and the microelectrode array which have excellent electric insulation and thermal isolation between a microheater and a silicon substrate, which has a small power consumption, which has high heating and cooling speed and which has no corrosion.

摘要翻译： 本发明涉及一种微电极，微电极阵列，以及可以控制温度的微电极的制造方法。 微电极包括形成在用于热隔离的硅衬底中的密封空腔，形成在密封空腔上的微加热器和由微加热器间接加热的电极。 根据本发明，可以通过CMOS工艺制造微电极和微电极阵列，其在微加热器和硅基板之间具有优异的电绝缘和热隔离，其功耗小，具有高的加热和冷却速度 没有腐蚀。