利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

46 条记录 (耗时 0.021 秒)

    Methods and kit for hybridization anaylsis using peptide nucleic acid probes
    1.
    发明申请
    Methods and kit for hybridization anaylsis using peptide nucleic acid probes 审中-公开
    使用肽核酸探针进行杂交分析的方法和试剂盒

    公开(公告)号：US20050053944A1

    公开(公告)日：2005-03-10

    申请号：US10636343

    申请日：2003-08-07

    申请人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

    发明人： Martin Fuchs ,  Michael Egholm ,  Heather O'Keefe ,  Xian-Wei Yao

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6813 ,  G01N27/447 ,  G01N33/566 ,  C07K14/47

    CPC分类号： G01N27/44726 ,  C12Q1/6813 ,  G01N27/447 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2565/125 ,  C12Q2565/629 ,  C12Q2565/107

    摘要： A method composition and apparatus for the hybridization and separation of molecules having a desired target sequence in a sample by contacting a sample of single stranded nucleic acids with a detectable PNA probe having a sequence complementary to the target sequence so that the target sequence, if present, will hybridize with the detectable probe to form a detectable duplex, and then separating the duplex in a denaturing medium from unbound sample components by electrophoresis. The invention also relates to methods compositions and apparatus for the hybridization and separation of molecules having a desired target sequence in a mixed sample of single stranded nucleic acids and their complementary strands by contacting the sample with a detectable PNA probe.

    摘要翻译： 一种方法组合物和装置，用于通过使单链核酸样品与具有与靶序列互补的序列的可检测PNA探针接触来杂交和分离样品中具有所需靶序列的分子，使得靶序列（如果存在） 将与可检测的探针杂交以形成可检测的双链体，然后通过电泳将变性培养基中的双链体与未结合的样品组分分离。 本发明还涉及用于通过使样品与可检测的PNA探针接触来在单链核酸及其互补链的混合样品中杂交和分离具有所需靶序列的分子的方法组合物和装置。

    Protein imaging
    4.
    发明授权
    Protein imaging 失效
    蛋白成像

    公开(公告)号：US5756717A

    公开(公告)日：1998-05-26

    申请号：US448822

    申请日：1995-05-24

    申请人： Sandeep K. Paliwal ,  Timothy K. Nadler ,  Laszlo Varady ,  Fred E. Regnier

    发明人： Sandeep K. Paliwal ,  Timothy K. Nadler ,  Laszlo Varady ,  Fred E. Regnier

    IPC分类号： B01J20/32 ,  C07K1/22 ,  C08B37/00 ,  C07K1/16 ,  C07K1/36

    CPC分类号： B01J20/26 ,  B01J20/268 ,  B01J20/285 ,  B01J20/291 ,  C07K1/22 ,  B01D15/3852

    摘要： Disclosed are specifically designed binding, "protein imaged" sorbents which reversibly bind with high specificity and affinity a preselected macromolecule, specifically a protein. The sorbents define one or more cavities which have a binding surface complementary in shape to the molecular surface of the macromolecule and a plurality of positively and negatively charged chemical moieties spatially distributed in a mirror image and charge inverse of a subset of the ionizable groups on the molecular surface of the macromolecule. Also disclosed are methods of producing such sorbents, useful over a range of conditions, for both preparative and analytical chromatographic separations or for use in various types of analyses.

    摘要翻译： 公开了特异性设计的结合，“蛋白质成像”吸附剂，其以特异性和亲和力以预先选择的大分子，特别是蛋白质可逆地结合。 吸附剂限定一个或多个空腔，其具有与大分子的分子表面形状互补的结合表面，以及空间上分布在镜像中的多个带正电荷和带负电荷的化学部分，并且电离反应 分子表面的大分子。 还公开了制备在一系列条件下用于制备和分析色谱分离或用于各种类型分析的这种吸附剂的方法。

    Perfusive chromatography
    5.
    发明授权
    Perfusive chromatography 失效
    渗透色谱法

    公开(公告)号：US5605623A

    公开(公告)日：1997-02-25

    申请号：US375910

    申请日：1995-01-20

    申请人： Noubar B. Afeyan ,  Fred E. Regnier ,  Robert C. Dean, Jr.

    发明人： Noubar B. Afeyan ,  Fred E. Regnier ,  Robert C. Dean, Jr.

    IPC分类号： B01D15/08 ,  B01D15/34 ,  G01N30/52

    CPC分类号： B01J20/285 ,  B01D15/345 ,  B01J20/28092 ,  B01J20/28095 ,  B01J2220/54 ,  G01N2030/528

    摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.

    摘要翻译： 公开了色谱方法和基质几何形状，其允许溶质，特别是生物材料的混合物的高分辨率，高生产率分离。 该方法包括以高流速将流体通过专门设计的色谱基质。 这些基质定义了第一和第二相互连接的孔组，以及用于与第二组孔的构件流体连通的溶质相互作用的高表面积。 第一和第二组孔体现为例如颗粒内的颗粒和透孔之间的间隙。 孔的尺寸使得在可实现的高流体流速下，在两个孔组中都发生对流，并且对流流速超过第二孔组中溶质扩散的速率。 这种方法将对流和扩散质量传输与活性表面相耦合，并允许流体速度的增加而没有通常预期的带扩展。

    Analysis utilizing isoelectric focusing
    6.
    发明授权
    Analysis utilizing isoelectric focusing 失效
    使用等电聚焦的分析

    公开(公告)号：US5376249A

    公开(公告)日：1994-12-27

    申请号：US981814

    申请日：1992-11-25

    申请人： Noubar B. Afeyan ,  Fred E. Regnier

    发明人： Noubar B. Afeyan ,  Fred E. Regnier

    IPC分类号： G01N33/561 ,  G01N27/447 ,  B01D57/02

    CPC分类号： G01N27/44795 ,  G01N27/44747

    摘要： Disclosed are methods and apparatus for determining the presence of one or more analytes in a sample, wherein the presence of a complex of an analyte and an analyte-specific binding moiety is detected at a location in an elongate pH gradient corresponding to a predetermined isoelectric point of the complex in the gradient. An electric field applied across the elongate pH gradient prior to the detection of the complex transports the complex to the location in the pH gradient corresponding to the predetermined isoelectric point. The analyte-specific binding moiety preferably is provided with a detectable label such as a fluorescent label. A parameter, e.g., fluorescence intensity, indicative of the amount of the complex at the location in the pH gradient corresponding to the predetermined isoelectric point may be determined to quantitate the analyte.

    摘要翻译： 公开了用于确定样品中一种或多种分析物的存在的方法和装置，其中在对应于预定等电点的细长pH梯度中的位置处检测到分析物和分析物特异性结合部分的复合物的存在 的复合体在渐变。 在检测复合物之前跨越细长pH梯度施加的电场将络合物输送到对应于预定等电点的pH梯度中的位置。 分析物特异性结合部分优选地具有可检测标记如荧光标记。 可以确定指示对应于预定等电点的pH梯度中位置处的络合物的量的参数，例如荧光强度，以定量分析物。

    Molecular imaging
    7.
    发明授权
    Molecular imaging 失效
    分子成像

    公开(公告)号：US5372719A

    公开(公告)日：1994-12-13

    申请号：US860450

    申请日：1992-03-30

    申请人： Noubar B. Afeyan ,  Laszlo Varady ,  Fred Regnier

    发明人： Noubar B. Afeyan ,  Laszlo Varady ,  Fred Regnier

    IPC分类号： B01J20/285 ,  B01D15/08 ,  B01J20/22 ,  B01J20/30 ,  B01J20/32 ,  G01N30/88 ,  G01N33/543 ,  G01N33/545

    CPC分类号： G01N33/54353 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3285 ,  G01N33/545 ,  G01N2600/00

    摘要： Disclosed are chemically-produced specific binding, "molecular imaged" sorbents which reversibly bind a preselected macromolecule by spacially matched multipoint interactions between functional groups synthesized on the surface of the sorbent and functional groups on the surface of the macromolecule. Also disclosed are methods of producing such sorbents. The sorbents typically are high surface area solids comprising surface binding regions which have charged groups, metal coordinating groups, hydrophobic moities, or various combination thereof anchored thereto and spaced in the mirror image of complementary interactive groups on a surface of the macromolecule.

    摘要翻译： 公开了化学产生的特异性结合，“分子成像”吸附剂，其通过在吸附剂表面上合成的官能团和大分子表面上的官能团之间的空间匹配的多点相互作用可逆地结合预选的大分子。 还公开了生产这种吸附剂的方法。 吸附剂通常是高表面积固体，其包含表面结合区域，其具有带电荷基团，金属配位基团，疏水性单元或其锚定到其上的各种组合并且在大分子表面上的互补相互作用基团的镜像中隔开。