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公开(公告)号：US11232851B2
公开(公告)日：2022-01-25
申请号：US15631832
申请日：2017-06-23
发明人： Yang Yang , Sophie Paquerault , Bradley Scott Denney , Lance Charlton , Jeanette Paek , Attaullah Seikh , Vyshnnavi Parthasarathy , Ken Pearson , Hung Huang , Tejinder Uppal
IPC分类号： G16B25/00 , G01N25/04 , C12Q1/68 , G01N35/00 , G16B45/00 , G16B50/00 , G01N25/48 , G16B25/20 , G16B5/30 , B01L3/00 , C12Q1/6851
摘要： The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).
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公开(公告)号：US10363558B2
公开(公告)日：2019-07-30
申请号：US14833939
申请日：2015-08-24
发明人： Ivor T. Knight , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
摘要： The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
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公开(公告)号：US10266873B2
公开(公告)日：2019-04-23
申请号：US13955749
申请日：2013-07-31
发明人： Hongye Liang , Kenton C. Hasson
摘要： This invention relates to systems and methods for imaging sample materials within a microfluidic device during an assay reaction process. In accordance with certain aspects of the invention, images are formed with a pixel array and a region of interest (“ROI”) is defined within the pixel array. Image values, such as fluorescent intensity, can be computed as averages of individual pixel values within the ROI. Where the ROI is subject to non-uniform conditions, such as non-uniform heating, the ROI can be divided into sub-ROIs which are sufficiently small that the condition is uniform within the sub-ROI.
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4.发明申请公开(公告)号：US20180311661A1
公开(公告)日：2018-11-01
申请号：US15997501
申请日：2018-06-04
摘要： At least one exemplary embodiment of the invention is directed to a molecular diagnostic device that comprises a cartridge configured to eject samples comprising genomic material into a microfluidic chip that comprises an amplification area, a detection area, and a matrix analysis area.
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公开(公告)号：US09919314B2
公开(公告)日：2018-03-20
申请号：US14923058
申请日：2015-10-26
IPC分类号： B01L3/00 , B01L7/00 , F28F9/02 , C12M1/40 , C12P19/34 , G01N35/08 , C07H1/06 , C07H21/04 , G01N35/00
CPC分类号： B01L7/52 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/041 , B01L2300/06 , B01L2300/18 , B01L2300/1805 , B01L2300/1838 , B01L2300/1844 , B01L2300/1883 , B01L2300/1894 , C12P19/34 , F28F9/02 , G01N35/08 , G01N2035/00346 , Y02P20/129
摘要： Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.
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公开(公告)号：US20180011969A1
公开(公告)日：2018-01-11
申请号：US15631794
申请日：2017-06-23
发明人： Yang Yang , Bradley Scott Denney , Attaullah Seikh
CPC分类号： G16B40/00 , C12Q1/6844 , G01N21/6428 , G01N25/04 , G01N2021/6439 , G06F17/18 , G16B20/20 , G16B40/30 , C12Q2527/107 , C12Q2531/113 , C12Q2537/165 , C12Q2561/113 , C12Q2563/107
摘要： The present invention relates to methods and systems for the analysis of nucleic acids present in biological samples, and more specifically, relates to clustering melt curves derived from high resolution thermal melt analysis performed on a sample of nucleic acids, the resulting clusters being usable, in one embodiment, for analyzing the sequences of nucleic acids and to classify their genotypes that are useful for determining the identity of the genotype of a nucleic acid that is present in a biological sample.
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7.发明申请公开(公告)号：US20170327867A1
公开(公告)日：2017-11-16
申请号：US15389372
申请日：2016-12-22
发明人： Abhijit Dohale , Arvind Virmani , Brian Scrivens , Christopher Sneeder , Denis Alias , George Maltezos , Hanyoup Kim , Harini Shandilya , Hongye Liang , Jason Zsak , Johnathan Stuart Coursey , Kenton C. Hasson , Melissa Gosse , Shulin Zeng , Yasuyuki Numajiri , Makoto Ogusu , Yoichi Murakami , Kunihiro Sakai
CPC分类号： C12Q1/6806 , B01L3/502 , B01L3/5021 , B01L3/502715 , B01L3/508 , B01L7/52 , B01L2200/0605 , B01L2200/0684 , B01L2200/16 , B01L2300/042 , B01L2300/0654 , B01L2300/0681 , B01L2300/087 , B01L2300/0896 , B01L2300/123 , B01L2300/168 , B01L2300/1827 , B01L2400/0478 , B01L2400/0487 , B01L2400/0605 , B01L2400/0683 , B01L2400/0688 , C12Q1/686 , G01N1/40
摘要： The present invention relates to sample-to-answer systems, devices, cartridges, and method of using the same for detecting the presence of microorganisms in a sample, such as bacteria.
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公开(公告)号：US09766139B2
公开(公告)日：2017-09-19
申请号：US13847885
申请日：2013-03-20
CPC分类号： G01K15/005
摘要： The present invention, in one aspect, provides systems and methods for using a single slug or multiple slugs containing one or more calibrators to determine a relationship between temperature and an electrical characteristic of the thermal sensor for use in connection with calibrating thermal sensors. In some embodiments, the present invention uses the described calibrator systems and methods to calibrate thermal control elements on a microfluidic device. In non-limiting embodiment, the calibrator can be one or more of droplets, plugs, slugs, segments or a continuous flow of any appropriate solution that, when heated, yields a thermal response profile with a plurality of features (e.g., maxima, minima, inflection points, linear regions, etc.).
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9.发明申请公开(公告)号：US20170157607A1
公开(公告)日：2017-06-08
申请号：US15436118
申请日：2017-02-17
发明人： Kenton C. HASSON , Gregory A. DALE
CPC分类号： B01L7/525 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L2200/10 , B01L2200/143 , B01L2300/0627 , B01L2300/0654 , B01L2300/0829 , B01L2300/18 , B01L2300/1811 , B01L2300/1822 , B01L2300/1827 , B01L2300/1877 , B01L2400/0487 , C12Q1/686 , G01N21/71 , G01N35/08 , G06T7/0012 , G06T7/11 , G06T2207/10004 , G06T2207/30072 , Y10T436/115831 , Y10T436/117497 , C12Q2565/629 , C12Q2561/113
摘要： The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. A method according to one embodiment of the invention may include the steps of: forcing a sample of a solution containing real-time PCR reagents to move though a channel; and while the sample is moving through an analysis region of the channel, performing the steps of: (a) cycling the temperature of the sample until the occurrence of a predetermined event; (b) after performing step (a), causing the sample's temperature to gradually increase from a first temperature to a second temperature; and (c) while the step of gradually increasing the sample's temperature is performed, using an image sensor to monitor emissions from the sample.
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公开(公告)号：US20160341605A1
公开(公告)日：2016-11-24
申请号：US15049881
申请日：2016-02-22
CPC分类号： G01K7/16 , B01L3/5027 , B01L7/525 , B01L2300/0816 , B01L2300/1827 , G01K3/10 , G06F17/12
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
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