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    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS
    1.
    发明申请
    MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS 有权

    公开(公告)号：US20220383983A1

    公开(公告)日：2022-12-01

    申请号：US17831648

    申请日：2022-06-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd REARICK ,  Jonathan SCHULTZ

    IPC: G16B40/10 ,  G16B40/00 ,  C12Q1/6869

    Abstract: Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    4.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开

    公开(公告)号：US20160011145A1

    公开(公告)日：2016-01-14

    申请号：US14863718

    申请日：2015-09-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: G01N27/414 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    5.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开
    用于生物和化学分析的集成传感器阵列

    公开(公告)号：US20150197797A1

    公开(公告)日：2015-07-16

    申请号：US14599948

    申请日：2015-01-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

    Abstract translation: 公开了一种在电路支撑衬底中包括化学场效应晶体管阵列的装置。 晶体管阵列在其表面上设置有能够从样品流体中保留化学或生物样品的样品保持区阵列。 晶体管阵列具有10μm或更小的间距，并且样品保持区位于至少一个化学场效应晶体管上，该化学场效应晶体管被配置为产生与该样品中的化学或生物样品的特征相关的至少一个输出信号 - 区域。

    Windowed Sequencing
    6.
    发明申请
    Windowed Sequencing 审中-公开
    窗口排序

    公开(公告)号：US20140336063A1

    公开(公告)日：2014-11-13

    申请号：US13891023

    申请日：2013-05-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd REARICK ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  Chris PAPALIAS ,  Kim L. JOHNSON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  G16B30/00

    Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

    Abstract translation: 在一个实现中，描述了一种方法。 该方法包括确定传感器阵列的传感器的操作特性。 该方法还包括基于组中的传感器的操作特性来选择阵列中的一组传感器。 该方法还包括使得能够读出所选择的组中的传感器。 所述方法还包括接收来自所述使能的传感器的输出信号，所述输出信号指示在所述传感器阵列的传感器附近发生的化学反应。

    FLUIDICS SYSTEMS FOR SEQUENTIAL DELIVERY OF REAGENTS
    9.
    发明申请
    FLUIDICS SYSTEMS FOR SEQUENTIAL DELIVERY OF REAGENTS 有权

    公开(公告)号：US20210379588A1

    公开(公告)日：2021-12-09

    申请号：US17304452

    申请日：2021-06-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  David MARRAN

    IPC: B01L3/00 ,  C12Q1/6869 ,  G01N27/447

    Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.

    CENTRIFUGE AND METHOD FOR LOADING A DEVICE
    10.
    发明申请
    CENTRIFUGE AND METHOD FOR LOADING A DEVICE 有权

    公开(公告)号：US20210139949A1

    公开(公告)日：2021-05-13

    申请号：US17027206

    申请日：2020-09-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

    IPC: C12Q1/6806 ,  B04B13/00 ,  B04B5/04 ,  G01N35/00 ,  G01N35/10 ,  C12Q1/6869

    Abstract: A sample preparation apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. In addition, the sample preparation apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles, as well as a centrifuge for loading such particles onto a sensor array. The sample preparation apparatus can further include receptacles for holding containers of reagent solutions.

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