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公开(公告)号：US07217559B2
公开(公告)日：2007-05-15
申请号：US10489883
申请日：2002-09-18
申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
发明人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
CPC分类号： C12N9/0073 , C12N1/20 , C12P17/08 , C12R1/40
摘要： The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
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公开(公告)号：US07462447B2
公开(公告)日：2008-12-09
申请号：US10467033
申请日：2002-02-01
CPC分类号： C07K14/705 , A61K38/00 , C07K14/47 , C07K14/51 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158
摘要： The invention provides mutant SH3-binding protein (SH3BP2) nucleic acids, polypeptides, and agents which selectively bind to the mutant SH3BP2 molecules and which do not bind to the wild type SH3BP2 molecules. Methods for selecting agents which inhibit mutant SH3BP2 expression, as well as diagnostic and therapeutic methods which utilize the mutant SH3BP2 molecules for diagnosing and treating disorders of bone homeostasis, also are provided.
摘要翻译： 本发明提供了选择性结合突变体SH3BP2分子并且不结合野生型SH3BP2分子的突变SH3结合蛋白（SH3BP2）核酸，多肽和试剂。 还提供了选择抑制突变体SH3BP2表达的药剂的方法，以及利用突变型SH3BP2分子诊断和治疗骨稳态障碍的诊断和治疗方法。
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公开(公告)号：US07273705B2
公开(公告)日：2007-09-25
申请号：US10704707
申请日：2003-11-12
申请人： Yung-Chiang Chung
发明人： Yung-Chiang Chung
CPC分类号： B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2400/0463 , C12Q1/6806 , G01N1/34 , C12Q2565/629 , C12Q2527/107
摘要： An RNA separation method includes injecting a reaction solution into a reaction region which contains a covalent linking of specific RNA probe for hybridizing with the reaction solution; adjusting a moderate hybridization temperature in the reaction region; moving the remainder of the reaction solution to a transition region by using a pneumatic actuator; adding a buffer solution to the reaction region, and heating the reaction region to a moderate denature temperature by a heating apparatus; moving a waste solution which is product produced from the reaction region after denature to a waste region by using the pneumatic actuator; and moving the remainder solution back to the reaction region by the pneumatic actuator.
摘要翻译： RNA分离方法包括将反应溶液注入含有与反应溶液杂交的特异性RNA探针的共价连接的反应区域; 调节反应区域中的中等杂交温度; 通过使用气动致动器将反应溶液的剩余部分移动到过渡区域; 向反应区域加入缓冲溶液，并通过加热装置将反应区域加热至适度的变性温度; 通过使用气动执行机构，将从变性后的反应区域产生的产品的废液移动到废弃区域; 并通过气动致动器将剩余溶液移回反应区域。
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4.发明授权Method of determining the nucleotide sequence of oligonucleotides and DNA molecules 有权确定寡核苷酸和DNA分子核苷酸序列的方法公开(公告)号：US08263364B2
公开(公告)日：2012-09-11
申请号：US13008303
申请日：2011-01-18
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6825 , C12Q2533/101 , C12Q2563/103 , C12Q2563/107 , C12Q2563/116 , C12Q2565/301 , C12Q2565/629 , G01N33/573 , G01N33/6863
摘要： The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
摘要翻译： 本发明涉及一种用于分析核酸序列的新方法，该方法基于DNA聚合酶催化的四个核苷酸碱基中的每一个（在微流体系统中单独提供和连续供给）中并入到包含模板系统的反应池中 包含未知序列的DNA片段和寡核苷酸引物。 将核苷酸碱基掺入模板系统可以通过多种方法中的任何一种检测，包括但不限于荧光和化学发光检测。 或者，可以使用通过使用热电堆，热敏电阻和折射率测量将核苷酸并入扩展模板系统产生的热的微量热法测定来检测延伸反应。
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5.发明授权
公开(公告)号：US5736330A
公开(公告)日：1998-04-07
申请号：US542401
申请日：1995-10-11
申请人： R. Jerrold Fulton
发明人： R. Jerrold Fulton
IPC分类号： C12Q1/68 , G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543 , C12P1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： G01N33/54313 , C12Q1/6827 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149
摘要： A method for the analysis of DNA sequences and PCR products comprises the steps of constructing an oligonucleotide-labeled bead set, and labeled complementary probe, and exposing the bead set and probe to a DNA fragment or PCR product under hybridizing conditions and analyzing the combined sample/bead set by flow cytometry. Flow cytometric measurements are used to classify beads within an exposed bead set to determine the presence of identical or nonidentical sequences within the test sample. The inventive technology enables the rapid analysis of DNA sequences and detection of point mutations, deletions and/or inversions while also reducing the cost and time for performing genetic assays.
摘要翻译： 分析DNA序列和PCR产物的方法包括以下步骤：在杂交条件下构建寡核苷酸标记的珠粒组和标记的互补探针，并将珠组和探针暴露于DNA片段或PCR产物，并分析组合的样品 /流式细胞仪设定的珠粒。 流式细胞术测量用于对暴露的珠粒组中的珠粒进行分类，以确定测试样品中相同或非相似序列的存在。 本发明的技术使得能够快速分析DNA序列并检测点突变，缺失和/或逆转，同时还降低了进行基因测定的成本和时间。
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公开(公告)号：US10150990B2
公开(公告)日：2018-12-11
申请号：US12421188
申请日：2009-04-09
IPC分类号： C12P1/68 , C12P19/34 , C12N9/00 , G01N31/00 , G01N33/00 , C07H21/04 , C07H21/00 , C12Q1/6858 , C12Q1/686
摘要： The present application provides polynucleotides comprising 5′-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5′-tail sequence segments. Cleavage of amplification products at the bond immediately 3′ to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.
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公开(公告)号：US07635578B2
公开(公告)日：2009-12-22
申请号：US11894808
申请日：2007-08-20
申请人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号：US07425434B2
公开(公告)日：2008-09-16
申请号：US11717247
申请日：2007-03-13
申请人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
发明人： Hiroaki Iwaki , Yoshie Hasegawa , Peter C. K. Lau
CPC分类号： C12N9/0073 , C12N1/20 , C12P17/08 , C12R1/40
摘要： The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
摘要翻译： 本发明涉及恶臭假单胞菌（称为HI-70）的新菌株，并且从所述菌株中分离，克隆和测序来自所述菌株的环十二酮单加氧酶编码基因（命名为cdnB）。 本发明还涉及新的环十二酮单加氧酶和使用环十二酮单加氧酶编码基因的方法。
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公开(公告)号：US6020130A
公开(公告)日：2000-02-01
申请号：US945734
申请日：1997-10-28
申请人： Larry Gold , Sumedha Javasena
发明人： Larry Gold , Sumedha Javasena
CPC分类号： C12Q1/6811 , C12N15/115 , C12Q1/6848 , C12Y207/07007 , C12N2310/16 , G01N2333/96433 , Y10S435/81
摘要： This invention discloses high-affinity oligonucleotide ligands to the thermostable Taq polymerase and Tth polymerase. Specifically, this invention discloses DNA ligands having the ability to bind to the Taq and Tth polymerases and the methods for obtaining such ligands. The ligands are capable of inhibiting polymerases at ambient temperatures.
摘要翻译： PCT No.PCT / US96 / 09451 Sec。 371日期1997年10月28日第 102（e）1997年10月28日PCT PCT 1996年6月5日PCT公布。 出版物WO96 / 41010 日期1996年12月19日本发明公开了耐热Taq聚合酶和Tth聚合酶的高亲和力寡核苷酸配体。 具体地，本发明公开了具有结合Taq和Tth聚合酶的能力的DNA配体和获得这种配体的方法。 配体能够在环境温度下抑制聚合酶。
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