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    Organs-on-chips as a platform for epigenetics discovery
    95.
    发明授权
    Organs-on-chips as a platform for epigenetics discovery 有权

    公开(公告)号：US12036548B2

    公开(公告)日：2024-07-16

    申请号：US16997509

    申请日：2020-08-19

    申请人： EMULATE, INC.

    发明人： Catherine Karalis ,  Ville Kujala

    IPC分类号： B01L3/00 ,  C12M3/06 ,  C12Q1/6809 ,  G01N33/50 ,  C12M1/12

    CPC分类号： B01L3/5027 ,  C12M23/16 ,  C12Q1/6809 ,  G01N33/5023 ,  G01N33/5064 ,  G01N33/5091 ,  B01L2300/0819 ,  B01L2400/0487 ,  C12M25/02

    摘要： The present invention relates to microfluidic fluidic devices, methods and systems for use in identifying epigenetic signatures in a range of sample types, e.g., cells established on a “chip” (including but not limited to single cell samples, cell populations, C cell layers and whole tissues, such as a biopsy), immune cells, cfDNA, exosomes, and the like. More specifically, in some embodiments, a microfluidic chip containing a sample is contacted with a test compound (e.g. DNA altering test compound, an RNA expression altering test compound, etc.) for use in providing a diagnostic epigenetic signature for that type of sample (or cell type) exposed to that specific test compound. In some embodiments, after contact with a test compound, effluent fluids (e.g. fluids exiting the “chip” that contacted the cells) are derived for testing as a “virtual blood draw.” In some embodiments, epigenetic signatures include (but are not limited to) identifying specific combinations of modifications of chromosomes and specific modifications of DNA.

    Physiology and pathophysiology of human gut: intestine-on-chip
    99.
    发明授权
    Physiology and pathophysiology of human gut: intestine-on-chip 有权

    公开(公告)号：US11566231B2

    公开(公告)日：2023-01-31

    申请号：US16825213

    申请日：2020-03-20

    申请人： EMULATE, INC.

    发明人： Athanasia Apostolou ,  Antonio Varone ,  Magdalena Kasendra ,  Raymond Luc

    IPC分类号： C12N5/071 ,  C12M3/06 ,  C12M1/12 ,  G01N1/30

    摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

    CONTROLLING PRESSURE
    100.
    发明申请
    CONTROLLING PRESSURE 有权

    公开(公告)号：US20230022203A1

    公开(公告)日：2023-01-26

    申请号：US17950422

    申请日：2022-09-22

    申请人： EMULATE, Inc

    发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Jose Fernandez-Alcon

    IPC分类号： C12M3/06 ,  B01L9/00 ,  C12M1/42 ,  C12N5/071 ,  C12M1/34 ,  A01N1/02 ,  B01L3/00 ,  C12M1/00 ,  C12M3/00 ,  C12M1/36

    摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.