公开(公告)号：US20080160634A1

公开(公告)日：2008-07-03

申请号：US11646601

申请日：2006-12-28

申请人： Xing Su ,  Kai Wu ,  David J. Liu

发明人： Xing Su ,  Kai Wu ,  David J. Liu

IPC分类号： G01N33/566 ,  G01N33/553

CPC分类号： G01N27/74 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00655 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/043 ,  B01L2400/0439 ,  B03C1/032 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N27/745 ,  G01R33/1269

摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises multiple zones such as a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

摘要翻译： 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括多个区域，例如样品区域，清洁区域和检测区域。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

公开(公告)号：US20080160622A1

公开(公告)日：2008-07-03

申请号：US11647566

申请日：2006-12-29

申请人： Xing Su ,  David J. Liu ,  Kenneth B. Swartz ,  Kai Wu ,  Mineo Yamakawa

发明人： Xing Su ,  David J. Liu ,  Kenneth B. Swartz ,  Kai Wu ,  Mineo Yamakawa

IPC分类号： G01N33/48 ,  B01J19/00 ,  H01R43/00 ,  G01N1/00

CPC分类号： G01N1/4044 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0638 ,  B01L2400/086 ,  G01N35/0098 ,  G01N35/08 ,  G01N2015/0038 ,  G01N2015/0092 ,  H01F7/20 ,  H01F7/206 ,  H01F2007/068 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T29/49117 ,  Y10T29/4913 ,  Y10T29/49155 ,  Y10T436/25

摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

摘要翻译： 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微机架阵列机械可移动的永磁体在功能上耦合到流体网络，其可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

公开(公告)号：US07361821B2

公开(公告)日：2008-04-22

申请号：US10251152

申请日：2002-09-20

申请人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.

摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含连接至一个或多个纳米糖基的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米线可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米颗粒或量子点。 当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

公开(公告)号：US07297778B2

公开(公告)日：2007-11-20

申请号：US10264945

申请日：2002-10-04

申请人： Hajime Matsuzaki ,  Xing Su ,  Sean Walsh ,  Giulia Kennedy ,  Rui Mei

发明人： Hajime Matsuzaki ,  Xing Su ,  Sean Walsh ,  Giulia Kennedy ,  Rui Mei

IPC分类号： C07H21/00 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/683 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q2600/156

摘要： The presently claimed invention provides for novel methods and kits for analyzing a collection of target sequences in a nucleic acid sample. A sample is amplified under conditions that enrich for a subset of fragments that includes a collection of target sequences. The invention further provides for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the collection of target sequences for particular characteristics, such as, for example, the presence or absence of one or more polymorphisms.

摘要翻译： 本发明提供了用于分析核酸样品中靶序列的集合的新颖方法和试剂盒。 在富集包括靶序列集合的片段子集的条件下扩增样品。 本发明进一步提供了通过与阵列杂交来分析上述样品，其可以被特别设计成询问用于特定特征的靶序列的收集，例如存在或不存在一个或多个多态性。

公开(公告)号：US20070231790A1

公开(公告)日：2007-10-04

申请号：US11394156

申请日：2006-03-31

申请人： Xing Su

发明人： Xing Su

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/554 ,  C12M3/00

CPC分类号： G01N33/54366 ,  C12Q1/6825 ,  C12Q2537/143 ,  C12Q2565/607

摘要： The application relates to a device and method for performing analyte detection, especially biomolecule detection. The device and method combine photo-induced charge separation in label materials and field effect transistors as sensors, resulting in more sensitive, specific and/or selective detections of biomolecules in multiplex assays, such as immunoassays and DNA microarray assays. The embodiments of the invention also encompass a device and method that comprise an array of electrical sensors, such as field effect transistors, and binding complexes for simultaneous multiplex detection of analytes.

摘要翻译： 本申请涉及用于进行分析物检测，特别是生物分子检测的装置和方法。 该装置和方法将标签材料和场效应晶体管中的光感应电荷分离结合为传感器，导致在多重测定（例如免疫测定和DNA微阵列测定）中更灵敏，特异和/或选择性地检测生物分子。 本发明的实施例还包括包括诸如场效应晶体管的电传感器阵列的装置和方法，以及用于同时多次检测分析物的结合复合物。

公开(公告)号：US20060234248A1

公开(公告)日：2006-10-19

申请号：US11081772

申请日：2005-03-15

申请人： Lei Sun ,  Xing Su ,  Mineo Yamakawa ,  Zhang Jingwu ,  Narayan Sundararajan

发明人： Lei Sun ,  Xing Su ,  Mineo Yamakawa ,  Zhang Jingwu ,  Narayan Sundararajan

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： G01N21/658 ,  B82Y30/00 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

摘要： Metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. The nanoclusters may be further functionalized, for example, with coatings and layers, such as adsorption layers, metal coatings, silica coatings, probes, and organic layers. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

摘要翻译： 提供能够从其中并入的有机拉曼活性分子提供增强的拉曼信号的金属纳米团簇。 纳米团簇可以进一步官能化，例如具有涂层和层，例如吸附层，金属涂层，二氧化硅涂层，探针和有机层。 纳米团簇通常被称为COIN（复合有机无机纳米粒子），并且能够作为敏感的报告物进行分析物检测。 可以将多种有机拉曼活性化合物和化合物的混合物掺入纳米团簇中。

公开(公告)号：US20060216729A1

公开(公告)日：2006-09-28

申请号：US11312188

申请日：2005-12-19

申请人： Lei Sun ,  Xing Su

发明人： Lei Sun ,  Xing Su

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/682 ,  G01N21/658 ,  C12Q2565/632 ,  C12Q2565/518 ,  C12Q2531/125 ,  C12Q2563/179

摘要： The present methods, compositions and systems are concerned with biomolecule 130 detection, identification and/or quantification by rolling circle amplification (RCA) and Raman detection. In particular embodiments of the invention, the RCA is exponential RCA or linear RCA. In some embodiments of the invention, the Raman detection is SERS or SERRS. The circular DNA template 150, 210, 310 to be amplified may comprise one or more polythymidine 320 residues, resulting in amplification products 170, 230, 250, 330, 410 containing multiple polyadenylate 340, 420 residues. The polyadenylates 340, 420 may be directly detected by Raman detection. Alternatively, one or more Raman labels may be incorporated into the amplification products 170, 230, 250, 330, 410 to facilitate Raman detection. Because of the amplification produced by LRCA or ERCA and the enhanced Raman signal produced by multiple polyadenylates 340, 420 and/or Raman labels, detection of single copy biomolecules 130 is feasible using the disclosed methods, compositions and/or systems.

摘要翻译： 本方法，组合物和系统涉及通过滚环扩增（RCA）和拉曼检测的生物分子130检测，鉴定和/或定量。 在本发明的特定实施例中，RCA是指数RCA或线性RCA。 在本发明的一些实施方案中，拉曼检测是SERS或SERRS。 要扩增的环状DNA模板150,210,310可以包含一个或多个多胸苷320残基，导致扩增产物170,230,250,330,410含有多个聚腺苷酸340,420个残基。 多聚腺苷酸酯340,420可以通过拉曼检测直接检测。 或者，可以将一个或多个拉曼标记掺入扩增产物170,230,250,330,410以促进拉曼检测。 由于由LRCA或ERCA产生的扩增和由多个多腺苷酸340,420和/或拉曼标记产生的增强的拉曼信号，使用所公开的方法，组合物和/或系统检测单拷贝生物分子130是可行的。

公开(公告)号：US07105301B2

公开(公告)日：2006-09-12

申请号：US10667776

申请日：2003-09-22

申请人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

发明人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/566 ,  G01N33/563

CPC分类号： C12Q1/6825 ,  B82Y5/00 ,  G01N33/54373 ,  G01N2800/52 ,  Y10S977/924 ,  C12Q2565/501

摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

摘要翻译： 本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。 在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。 将探针与分析物结合导致由检测单元检测到的悬臂的偏转。 可以应用平衡力将悬臂恢复到其原始位置。 平衡力可以是磁性的，电的或辐射的。 生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。 计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。 样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

公开(公告)号：US20060199193A1

公开(公告)日：2006-09-07

申请号：US11226696

申请日：2005-09-13

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Zhang Jingwu ,  Mineo Yamakawa ,  Val Dubin

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Zhang Jingwu ,  Mineo Yamakawa ,  Val Dubin

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6874 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00641 ,  B01J2219/00722 ,  B01L3/5085 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148

摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.

公开(公告)号：US07019828B2

公开(公告)日：2006-03-28

申请号：US10387080

申请日：2003-03-12

申请人： Xing Su ,  Lei Sun ,  Tae-Woong Koo ,  Selena Chan

发明人： Xing Su ,  Lei Sun ,  Tae-Woong Koo ,  Selena Chan

IPC分类号： G01J3/44

CPC分类号： G01N21/658 ,  B82Y10/00 ,  B82Y20/00

摘要： Briefly, in accordance with one embodiment of the invention, the intensity of the signals from surface enhanced Raman spectroscopy may be increased by using lithium chloride as an enhancer to activate a metallic structure used for surface enhanced Raman spectroscopy. The increased signal intensity may allow surface enhanced Raman spectroscopy to be utilized to detect individual analytes such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.

摘要翻译： 简而言之，根据本发明的一个实施例，可以通过使用氯化锂作为增强剂激活用于表面增强拉曼光谱的金属结构来增加来自表面增强拉曼光谱的信号的强度。 增加的信号强度可以允许使用表面增强拉曼光谱来检测单个分析物例如核苷酸，例如在DNA测序中，而不需要染料或放射性标记。