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133 条记录 (耗时 0.059 秒)

    pH INDUCED SILK GELS AND USES THEREOF
    101.
    发明申请
    pH INDUCED SILK GELS AND USES THEREOF 有权
    pH诱导丝光凝胶及其用途

    公开(公告)号：US20140134240A1

    公开(公告)日：2014-05-15

    申请号：US13933611

    申请日：2013-07-02

    申请人： TRUSTEES OF TUFTS COLLEGE

    发明人： David L. Kaplan ,  Tuna Yucel ,  Tim Jia-Ching Lo ,  Gary G. Leisk

    IPC分类号： A61K47/42 ,  A61L26/00 ,  A61K9/10 ,  A61K41/00

    CPC分类号： A61K47/42 ,  A61K9/10 ,  A61K41/00 ,  A61L26/0047 ,  A61L27/227 ,  A61L27/34 ,  A61L27/3604 ,  A61L27/38 ,  A61L27/52 ,  H01L41/193 ,  C08L89/00

    摘要： This invention provides for compositions, methods and devices for rapidly converting silk fibroin solution into a silk fibroin gel using direct application of voltage, in a process called electrogelation. The silk fibroin gel may be reversibly converted back to liquid form by applying reverse voltage or may be converted further to β-sheet structure by applying shear force or other treatments. The electrogelated silk may be used as an extracted bulk gel, spray or stream of gel for processing into materials or devices, or may be used as silk gel coating to devices. Active agents may be embedded in the silk gel for various medical applications. This invention also provides for methods and compositions for preparing adhesive silk pH-gels. For example, the method comprises reducing pH level of a silk fibroin solution to increase the bulk or local proton concentration of the silk fibroin solution, thereby forming adhesive silk gels.

    摘要翻译： 本发明提供了在称为电凝胶的方法中，使用直接施加电压将丝素蛋白溶液快速转化成丝素蛋白凝胶的组合物，方法和装置。 丝素蛋白凝胶可以通过施加反向电压而可逆地转化回液体形式，或者可以通过施加剪切力或其它处理而进一步转化为“薄片”结构。 电致凝胶丝可以用作提取的体积凝胶，喷雾或凝胶流加工成材料或装置，或者可以用作设备的丝凝胶涂层。 活性剂可以嵌入丝绸凝胶中用于各种医疗应用。 本发明还提供了制备粘性丝pH凝胶的方法和组合物。 例如，该方法包括降低丝素蛋白溶液的pH值以增加丝素蛋白溶液的体积或局部质子浓度，由此形成粘性丝凝胶。

    COVALENTLY IMMOBILIZED PROTEIN GRADIENTS IN THREE-DIMENSIONAL POROUS SCAFFOLDS
    102.
    发明申请
    COVALENTLY IMMOBILIZED PROTEIN GRADIENTS IN THREE-DIMENSIONAL POROUS SCAFFOLDS 审中-公开
    三维多孔蛋白质中共同固定的蛋白质降解

    公开(公告)号：US20130230491A1

    公开(公告)日：2013-09-05

    申请号：US13775328

    申请日：2013-02-25

    申请人： TRUSTEES OF TUFTS COLLEGE ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

    发明人： Charu Vepari ,  David L. Kaplan ,  Gordana Vunjak-Novakovic

    IPC分类号： C07K17/02 ,  C12N11/02

    CPC分类号： C07K17/02 ,  C07K17/04 ,  C12N11/02 ,  C12N11/04 ,  C12N11/06 ,  C12N11/10

    摘要： The invention provides a method for forming an immobilized agent gradient within a 3-dimensional porous scaffold. A 3-dimensional scaffold formed from a biocompatible material is provided. The surface of the scaffold and/or the agent is activated so as to allow binding of the agent to the scaffold. The activated scaffold is contacted with a solution containing the agent. Contact with the solution is maintained for a sufficient period of time to allow diffusion of the solution through a portion of the scaffold, thereby forming a desired gradient of the agent through the 3-dimensional scaffold.

    摘要翻译： 本发明提供了在三维多孔支架内形成固定化剂梯度的方法。 提供由生物相容性材料形成的3维支架。 支架和/或试剂的表面被活化，以允许试剂与支架结合。 活化的支架与含有该试剂的溶液接触。 保持与溶液接触足够的时间以允许溶液扩散通过支架的一部分，从而通过3维支架形成所需试剂的梯度。

    METHOD FOR FORMING INORGANIC COATINGS
    104.
    发明申请
    METHOD FOR FORMING INORGANIC COATINGS 审中-公开
    形成无机涂料的方法

    公开(公告)号：US20130177608A1

    公开(公告)日：2013-07-11

    申请号：US13768615

    申请日：2013-02-15

    申请人： TRUSTEES OF TUFTS COLLEGE

    发明人： David L. Kaplan ,  Chunmei Li

    IPC分类号： A61L27/22

    CPC分类号： A61L27/227 ,  A61L27/24 ,  A61L27/30 ,  A61L27/446 ,  A61L27/46 ,  Y10T428/24999 ,  Y10T428/2933 ,  Y10T428/31768 ,  Y10T442/10

    摘要： The present invention is directed to a method for forming an inorganic coating on a protein template. The method comprises contacting the template with an anionic polymer interface followed by an inorganic material for a sufficient period of time to allow mineralization of the inorganic material thus forming an inorganic coating on the template. Preferably, the coating is aligned.

    摘要翻译： 本发明涉及一种在蛋白质模板上形成无机涂层的方法。 该方法包括使模板与阴离子聚合物界面接触，然后使无机材料接触足够的时间以允许无机材料的矿化，从而在模板上形成无机涂层。 优选地，将涂层对准。

    POLYPEPTIDE MONOLITHS
    106.
    发明公开
    POLYPEPTIDE MONOLITHS 审中-公开

    公开(公告)号：US20240325609A1

    公开(公告)日：2024-10-03

    申请号：US18482421

    申请日：2023-10-06

    申请人： Trustees of Tufts College

    发明人： Fiorenzo G. Omenetto ,  David L. Kaplan ,  Benedetto Marelli ,  Chunmei Li

    IPC分类号： A61L27/56 ,  A61K9/50 ,  A61L27/22 ,  A61L27/46

    CPC分类号： A61L27/56 ,  A61L27/227 ,  A61L27/46 ,  A61K9/5052 ,  A61L2300/62

    摘要： The present application relates to amphiphilic polypeptide materials, methods for making and using the same. Provided amphiphilic polypeptide materials are water-based and manufactured using all aqueous processing. Provided materials exhibit a capacity to encapsulate and store biologically active molecules or macromolecules. Such biologically active molecules or macromolecules retain their structure and the biological activity so that these biologically active molecules or macromolecules are not materially degraded, reduced, and/or inhibited by processing steps or exposure. Provided materials possess unique mechanical and structural properties, including size, density, moldability and machinability.

    CULTURED TISSUE AND BIOREACTOR SYSTEMS AND METHODS FOR PRODUCTION THEREOF
    109.
    发明公开
    CULTURED TISSUE AND BIOREACTOR SYSTEMS AND METHODS FOR PRODUCTION THEREOF 审中-公开

    公开(公告)号：US20230284662A1

    公开(公告)日：2023-09-14

    申请号：US18041363

    申请日：2021-08-12

    申请人： Trustees of Tufts College

    发明人： David L. Kaplan ,  John Yuen ,  Natalie R. Rubio

    IPC分类号： A23L13/00 ,  C12M3/00 ,  C12M1/12 ,  C12M1/00 ,  C12N5/071

    CPC分类号： A23L13/00 ,  C12M21/08 ,  C12M25/14 ,  C12M23/58 ,  C12N5/0697 ,  C12N2513/00 ,  C12N2533/50

    摘要： The present disclosure relates to cultured tissue, methods for production of the cultured tissue, and a bioreactor system for production of the cultured tissue. In some embodiments, the production of the cultured tissue may involve, at a first bioreactor, feeding a fiber scaffold into a chamber containing culture media, seeding the chamber with precursor cells, and allowing the precursor cells to proliferate and differentiate on a surface of the fiber scaffold. At downstream bioreactors, the production of the cultured tissue may further involve twisting a plurality of the cell-laden fibers to provide a cell-laden yarn, and weaving or knitting the cell-laden yarn into a three-dimensional (3D) structure. In some embodiments, the cultured tissue may be whole muscle cultured meat composed of muscle cell-laden fibers and fat cell-laden fibers. The whole muscle cultured meat may have a structural organization and hierarchy that mimics natural skeletal muscle tissue.