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    Probe Based Nucleic Acid Detection
    131.
    发明申请
    Probe Based Nucleic Acid Detection 有权
    基于探针的核酸检测

    公开(公告)号：US20130045881A1

    公开(公告)日：2013-02-21

    申请号：US13467933

    申请日：2012-05-09

    Applicant: Kenneth J. Livak ,  Stacey N. Myers ,  Jun Wang ,  Xiaohui Wang

    Inventor： Kenneth J. Livak ,  Stacey N. Myers ,  Jun Wang ,  Xiaohui Wang

    IPC: G01N21/64 ,  C40B30/04

    CPC classification number: C12Q1/6818 ,  C12Q2525/151

    Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

    Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

    MULTIFUNCTIONAL PROBE-PRIMERS
    133.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：US20120288857A1

    公开(公告)日：2012-11-15

    申请号：US13366178

    申请日：2012-02-03

    Applicant: Kenneth J. Livak

    Inventor： Kenneth J. Livak

    IPC: C07H21/00 ,  G01N21/64 ,  C12Q1/68

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional “self-digesting” molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5′-5′ orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选的索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'方向连接的引物多核苷酸和探针寡核苷酸的多功能自消化分子探针。

    Universal Probe Assay Methods
    135.
    发明申请
    Universal Probe Assay Methods 有权
    通用探针测定方法

    公开(公告)号：US20120115143A1

    公开(公告)日：2012-05-10

    申请号：US13280214

    申请日：2011-10-24

    Applicant: Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    Inventor： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC: C12Q1/68

    CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物 。

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