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    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION
    28.
    发明申请
    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION 审中-公开
    DROPLET标记和放大的方法和系统

    公开(公告)号：US20170029813A1

    公开(公告)日：2017-02-02

    申请号：US15303893

    申请日：2015-04-17

    Applicant: President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusets General Hospital

    Inventor： David A. Weitz ,  Anthony John Iafrate ,  Zongli Zheng ,  Huidan Zhang

    IPC: C12N15/10

    CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfiuidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid “tags,” and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

    Abstract translation: 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的方法和系统。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微液滴。 通过将多个核酸“标签”并入可选地扩增核酸，例如在液滴内，液滴暴露于的条件可由核酸编码。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。

    VALVES AND OTHER FLOW CONTROL IN FLUIDIC SYSTEMS INCLUDING MICROFLUIDIC SYSTEMS
    30.
    发明申请
    VALVES AND OTHER FLOW CONTROL IN FLUIDIC SYSTEMS INCLUDING MICROFLUIDIC SYSTEMS 审中-公开
    流体系统中的阀门和其他流量控制，包括微流体系统

    公开(公告)号：US20160250638A1

    公开(公告)日：2016-09-01

    申请号：US15148955

    申请日：2016-05-06

    Applicant: President and Fellows of Harvard College

    Inventor： Adam R. Abate ,  David A. Weitz

    IPC: B01L3/00 ,  F16K99/00

    CPC classification number: B01L3/502738 ,  B01L3/0268 ,  B01L3/502746 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0636 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/082 ,  F16K99/0017 ,  F16K99/0026 ,  F16K99/0055 ,  Y10T436/2575

    Abstract: Articles and methods for controlling flow in fluidic systems, especially in microfluidic systems, are provided. In one aspect, a microfluidic system described herein includes a configuration such that the actuation of a single valve can allow the switching of fluids from a first fluid path (e.g., a first channel section) to a second fluid path (e.g., a second channel section). This may be achieved, for example, by incorporating a valve with a first channel section, which may have a lower hydrodynamic resistance than a second channel section prior to actuation of the valve. Actuation of the valve can cause only the hydrodynamic resistance of the first channel section to increase, thereby redirecting fluid flow into the second channel section (which now has a relatively lower hydrodynamic resistance). In some embodiments, the valve comprises a control channel for introducing a positive or reduced pressure, and is adapted to modulate fluid flow in an adjacent channel section by constricting or expanding the channel section. For example, the valve and/or the channel section may be formed in a flexible material and actuation of the valve may be achieved by applying a positive or reduced pressure to the valve to cause deformation of both the valve and the channel section. Another aspect of the invention includes articles and methods associated with manipulation of multiphase materials (e.g., dispersions). For instance, one or more valves may be combined with a flow focusing system so as to form droplets of different volumes and/or frequencies without the need to vary flow rates of the fluids when they are introduced into the fluidic system.

    Abstract translation: 提供了用于控制流体系统流动的文章和方法，特别是在微流体系统中。 在一个方面，本文所述的微流体系统包括使得单个阀的致动可以允许流体从第一流体路径（例如，第一通道部分）切换到第二流体路径（例如，第二通道 部分）。 这可以通过例如通过结合具有第一通道部分的阀来实现，该阀在致动阀之前可具有比第二通道部分更低的流体动力阻力。 阀的致动只能使第一通道部分的流体动力阻力增加，从而将流体流转向第二通道部分（其现在具有相对较低的流体动力阻力）。 在一些实施例中，阀包括用于引入正压或减压的控制通道，并且适于通过收缩或扩大通道部分来调制相邻通道部分中的流体流动。 例如，阀和/或通道部分可以形成为柔性材料，并且可以通过向阀施加正压或减压以使阀和通道部分变形来实现阀的致动。 本发明的另一方面包括与多相材料（例如分散体）的操作有关的制品和方法。 例如，一个或多个阀可以与流动聚焦系统组合，以便形成不同体积和/或频率的液滴，而不需要在将流体引入流体系统时改变流体的流速。

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