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    Screening assay for the detection of DNA-binding molecules
    32.
    发明授权
    Screening assay for the detection of DNA-binding molecules 失效
    检测DNA结合分子的筛选试验

    公开(公告)号：US5726014A

    公开(公告)日：1998-03-10

    申请号：US123936

    申请日：1993-09-17

    Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin

    Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin

    IPC: C12N15/09 ,  C07K14/035 ,  C07K14/47 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34 ,  G01N33/566

    CPC classification number: C12Q1/6811 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6883 ,  C12Q1/705

    Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

    Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

    DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof
    33.
    发明授权
    DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof 失效
    编码链霉亲和素的DNA，由此产生的链霉抗生物素蛋白，包括存在于链霉亲和素中的氨基酸序列的融合多肽及其用途

    公开(公告)号：US4839293A

    公开(公告)日：1989-06-13

    申请号：US833324

    申请日：1986-02-24

    Applicant: Charles R. Cantor ,  Richard Axel ,  Carlos Argarana

    Inventor： Charles R. Cantor ,  Richard Axel ,  Carlos Argarana

    IPC: C12N15/09 ,  C07K14/36 ,  C07K14/705 ,  C12N1/20 ,  C12N5/10 ,  C12P21/02 ,  C12R1/19 ,  C12R1/465 ,  C12R1/91

    CPC classification number: C07K14/36 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/22 ,  C07K2319/33 ,  Y10S930/30

    Abstract: DNA which encodes the polypeptide streptavidin has been isolated as a fragment 2 kb in length derived from a restriction endonuclease digestion of the chromosomal DNA of Streptomyces avidinii. The nucleic acid sequence of the gene and the amino acid sequence of the polypeptide have been determined. A fused gene has been prepared which comprises the streptavidin gene fused to a gene encoding the human LDL receptor. Expression of the gene fusion results in a fused streptavidin-human LDL receptor polypeptide. Methods are provided for using the fused gene to produce labeled, chemically modified proteins in vivo and to isolate a protein knowing only the nucleotide sequence of the gene encoding the protein.

    Abstract translation: 编码多肽链霉抗生物素蛋白的DNA已经被分离为长度为2kb的片段，其来源于阿维链霉菌的染色体DNA的限制性内切核酸酶消化。 已经确定了该基因的核酸序列和该多肽的氨基酸序列。 已经制备了融合基因，其包含与编码人LDL受体的基因融合的链霉亲和素基因。 基因融合的表达导致融合的链霉亲和素 - 人LDL受体多肽。 提供了使用融合基因在体内产生标记的，化学修饰的蛋白质并分离仅知道编码蛋白质的基因的核苷酸序列的蛋白质的方法。

    Quantification of Gene Expression
    34.
    发明申请
    Quantification of Gene Expression 有权
    基因表达的定量

    公开(公告)号：US20120028838A1

    公开(公告)日：2012-02-02

    申请号：US13223923

    申请日：2011-09-01

    Applicant: Charles R. Cantor ,  Chunming Din

    Inventor： Charles R. Cantor ,  Chunming Din

    IPC: C12Q1/68 ,  C40B30/10

    CPC classification number: C12Q1/6851 ,  C12Q2545/107 ,  C12Q2535/125 ,  C12Q2525/186

    Abstract: The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.

    Abstract translation: 本发明涉及使用与目的基因相比具有一个碱基差异的标准或“靶核酸序列”来测量样品中靶核酸量的方法。使用这种标准物 结合使用例如在突变位点上携带的碱基延伸反应来“提高”标准差和测试核酸样品的方法，允许以相同的效率扩增标准品和靶核酸并促进定量 的靶核酸。 此后，使用定量“增强”标准和靶核酸样品的手段来确定靶核酸的量。 在优选实施方案中，定量方法是质谱法。

    SUPRAMOLECULAR BIOCONJUGATES
    35.
    发明申请
    SUPRAMOLECULAR BIOCONJUGATES 审中-公开

    公开(公告)号：US20100255558A1

    公开(公告)日：2010-10-07

    申请号：US12435665

    申请日：2009-05-05

    Applicant: Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    Inventor： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

    IPC: C07K17/00 ,  C07K1/00 ,  C12N9/96

    CPC classification number: B82Y5/00 ,  A61K47/54 ,  A61K47/55 ,  A61K47/557 ,  A61K47/6949 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682

    Abstract: The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to a sequence within said plurality of first nucleic acids. To assemble a supramolecular bioconjugate, one or more sets of bioreactive agents are coupled to the plurality of mediators, forming a plurality of bioreactive complexes. The plurality of bioreactive complexes are hybridized to the plurality of first nucleic acids to form the supramolecular bioconjugate. Bioconjugates can be used to detect and isolate targets, to screen samples for targets such as antigens, to treat patients with multiple agents or to diagnose disorders in the form of a kit.

    Abstract translation: 本发明涉及超分子生物缀合物和组分和利用超分子生物缀合物的方法。 超分子生物缀合物包含多个第一核酸和多个介体，其中每个介体包含与所述多个第一核酸内的序列互补的第二核酸。 为了组装超分子生物缀合物，将一组或多组生物反应剂偶联至多个介体，形成多个生物反应性复合物。 多个生物反应性复合物与多个第一核酸杂交以形成超分子生物缀合物。 生物共轭物可用于检测和分离靶标，筛选靶标如抗原的样品，以治疗患有多种药物的患者或以试剂盒的形式诊断疾病。

    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity
    39.
    发明授权
    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity 失效
    具有生物识别特异性的重组链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白

    公开(公告)号：US06391590B1

    公开(公告)日：2002-05-21

    申请号：US07780717

    申请日：1991-10-21

    Applicant: Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    Inventor： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    IPC: C12N1562

    CPC classification number: C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

    Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

    Abstract translation: 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

    Sequence directed DNA binding molecules compositions and methods
    40.
    发明授权
    Sequence directed DNA binding molecules compositions and methods 失效
    序列定向DNA结合分子的组成和方法

    公开(公告)号：US06384208B1

    公开(公告)日：2002-05-07

    申请号：US09354947

    申请日：1999-07-15

    Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

    Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

    IPC: C07H2104

    CPC classification number: C12Q1/701 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6811 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/705 ,  H01L2924/14 ,  C12Q2525/161 ,  C12Q2525/131 ,  C12Q2537/161 ,  C12Q2522/101 ,  C12Q2521/301

    Abstract: The present invention defines a DNA: protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

    Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

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