公开(公告)号：US5728540A

公开(公告)日：1998-03-17

申请号：US468670

申请日：1995-06-06

申请人： Herve Perron ,  Tomas Dobransky ,  Fran.cedilla.ois Rieger ,  Bernard Mandrand

发明人： Herve Perron ,  Tomas Dobransky ,  Fran.cedilla.ois Rieger ,  Bernard Mandrand

IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K1/18 ,  C07K14/47 ,  C12Q1/02 ,  G01N33/50 ,  G01N33/566 ,  C12Q1/37 ,  C12N5/00 ,  C12Q1/04 ,  G01N33/567

CPC分类号： C07K14/4713 ,  G01N33/5091 ,  A61K38/00 ,  Y10S424/16 ,  Y10S435/964 ,  Y10S436/827 ,  Y10S436/828

摘要： Gliotoxic factor in the isolated or purified state, characterized in that it possesses toxic activity with respect to human or animal astrocytic cells, having the effect of a cytomorphological disorganization of their network of intermediate filaments and/or a degradation of the proteins of said intermediate filaments and/or cell death, in particular by apoptosis.

公开(公告)号：US5654143A

公开(公告)日：1997-08-05

申请号：US412229

申请日：1995-03-27

申请人： Fran.cedilla.ois Mallet ,  Guy Oriol ,  Bernard Mandrand

发明人： Fran.cedilla.ois Mallet ,  Guy Oriol ,  Bernard Mandrand

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6865

摘要： An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.

摘要翻译： RNA扩增方法特别适用于诊断细菌或病毒感染或遗传疾病和细胞分型。 该方法包括以下步骤：使含有RNA的溶液变性，在逆转录酶存在下合成来自合适引物的第一条cDNA链，使形成的异源双链变性，在DNA聚合酶存在下从第二引物合成第二条cDNA链，然后 使形成的cDNA进行足够数量的扩增循环。 所有的反应物和溶剂首先放在同一容器中，以提供避免污染风险的单一操作步骤。

公开(公告)号：US08133411B2

公开(公告)日：2012-03-13

申请号：US11921873

申请日：2006-06-30

申请人： Marie-Thérèse Charreyre ,  Bernard Mandrand ,  José Manuel Gaspar Martinho ,  Paula Relogio ,  José Paulo Sequeira Farinha

发明人： Marie-Thérèse Charreyre ,  Bernard Mandrand ,  José Manuel Gaspar Martinho ,  Paula Relogio ,  José Paulo Sequeira Farinha

IPC分类号： C09K11/08 ,  C09B69/10 ,  C07K17/08 ,  C07K17/10 ,  C12N11/06 ,  C08F2/38 ,  C08F22/40 ,  C08F8/00

CPC分类号： C08F8/30 ,  C08F2/38 ,  C08F8/34 ,  C08F220/36 ,  C09B69/103 ,  C09B69/109

摘要： The invention relates to fluorescent polymers soluble in an aqueous solution carrying at least 5 fluorophores which are distributed on the polymer and which exhibit the following properties: the fluorophores are water-soluble, the fluorophores are not self-associated in water at a concentration of less than or equal to 10−4 mol/l, preferably at a concentration of less than or equal to 10−3 mol/l, the fluorophores which are free in the aqueous solution have a molar extinction coefficient of greater than 1000 M−1·cm−1, preferably greater than 5000 M−1·cm−1, the fluorophores which are free in the aqueous solution have a quantum yield of greater than 0.3, preferably greater than 0.6, said polymers having a fluorescence amplification factor of greater than or equal to 0.35 per kg/mol of polymer, preferably greater than 0.45 per kg/mol of polymer.

摘要翻译： 本发明涉及可溶于携带至少5个荧光团的水溶液中的荧光聚合物，其分布在聚合物上并且具有以下性质：荧光团是水溶性的，荧光团在水中不是以相对较少的浓度自相关 10-4mol / l以上，优选浓度小于或等于10-3mol / l，在水溶液中游离的荧光团的摩尔消光系数大于1000M-1· cm-1，优选大于5000M-1·cm-1，所述水溶液中游离的荧光团的量子产率大于0.3，优选大于0.6，所述聚合物的荧光放大因子大于或等于 等于每kg / mol聚合物0.35，优选大于0.45 / kg / mol聚合物。

公开(公告)号：US08088910B2

公开(公告)日：2012-01-03

申请号：US12776893

申请日：2010-05-10

申请人： Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel ,  Frederic Bedin ,  Mireille Sodoyer ,  Catherine Ott ,  Francois Mallet ,  Herve Perron ,  Bernard Mandrand

发明人： Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel ,  Frederic Bedin ,  Mireille Sodoyer ,  Catherine Ott ,  Francois Mallet ,  Herve Perron ,  Bernard Mandrand

IPC分类号： C07H21/04 ,  A61K39/21

CPC分类号： C07K14/4713 ,  C07K14/005 ,  C12N2740/10022

摘要： An isolated polynucleotide having a nucleotide sequence selected from the group consisting of (a) SEQ ID NO: 21, (b) the full-length sequences encoding a polypeptide having a peptide sequence selected from the group consisting of SEQ ID NOs: 25 and 26, and (c) the full-length complementary sequences to the sequences set forth in (a) or (b).

摘要翻译： 一种分离的多核苷酸，其具有选自（a）SEQ ID NO：21，（b）编码具有选自SEQ ID NO：25和26的肽序列的多肽的全长序列的核苷酸序列 ，和（c）与（a）或（b）中列出的序列的全长互补序列。

公开(公告)号：US07932350B2

公开(公告)日：2011-04-26

申请号：US11463109

申请日：2006-08-08

申请人： Herve Perron ,  Frederic Beseme ,  Frederic Bedin ,  Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel ,  Colette Jolivet-Reynaud ,  Bernard Mandrand

发明人： Herve Perron ,  Frederic Beseme ,  Frederic Bedin ,  Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel ,  Colette Jolivet-Reynaud ,  Bernard Mandrand

IPC分类号： C07K14/005 ,  C07K14/15 ,  C07K14/00 ,  C07H23/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/51 ,  C12N2740/10022 ,  C12Q1/6883 ,  C12Q1/702

摘要： Viral material, in the isolated or purified state, in which the genome comprises a nucleotide sequence chosen from the group including sequences SEQ ID NO:46, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53 and SEQ ID NO:56, their complementary sequences and their equivalent sequences, in particular nucleotide sequences displaying, for any succession of 100 contiguous monomers, at least 50% and preferably at least 70% homology with the said sequences SEQ ID NO:46, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53 and SEQ ID NO:56, respectively, and their complementary sequences.

摘要翻译： 分离或纯化状态的病毒物质，其中基因组包含选自SEQ ID NO：46，SEQ ID NO：51，SEQ ID NO：52，SEQ ID NO：53和SEQ ID NO：53的核苷酸序列 SEQ ID NO：46，SEQ ID NO：56，其互补序列及其等同序列，特别是核苷酸序列，显示100个连续单体的任何连续序列，与所述序列SEQ ID NO：46，SEQ ID NO为至少50％，优选至少70％ ：51，SEQ ID NO：52，SEQ ID NO：53和SEQ ID NO：56及其互补序列。

公开(公告)号：US20100209987A1

公开(公告)日：2010-08-19

申请号：US12656821

申请日：2010-02-17

申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

IPC分类号： C12N13/00

CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

公开(公告)号：US20100145014A1

公开(公告)日：2010-06-10

申请号：US12634204

申请日：2009-12-09

申请人： Alain LAURENT ,  Philippe Sarra-Bournet ,  Olivier Aude ,  Paul Morgavi ,  Bernard Mandrand ,  Marc Cuzin

发明人： Alain LAURENT ,  Philippe Sarra-Bournet ,  Olivier Aude ,  Paul Morgavi ,  Bernard Mandrand ,  Marc Cuzin

IPC分类号： C07K1/04 ,  C07H1/00

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/00423 ,  B01J2219/00497 ,  B01J2219/00529 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  C07K1/04 ,  C07K1/045 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14 ,  C40B70/00

摘要： The present invention relates to an automated polymer synthesis apparatus for synthesizing a polymer chain onto a solid substrates by sequentially adding polymer building blocks as well as to a method for synthesizing polymers on solid substrates by sequentially reacting polymer building blocks with reactive groups. The invention further relates to a biochip comprising a solid substrate with reactive groups where biomolecules are attached to and the remaining reactive groups are transformed into chemically inert species.

摘要翻译： 本发明涉及一种用于通过依次加入聚合物结构单元将聚合物链合成到固体基质上的自动聚合物合成设备，以及通过使聚合物结构单元与反应性基团顺序反应的方法，在固体基质上合成聚合物的方法。 本发明还涉及一种生物芯片，其包含具有反应性基团的固体底物，其中生物分子连接到其上，并且剩余的反应性基团转化为化学惰性的物质。

公开(公告)号：US20080290321A1

公开(公告)日：2008-11-27

申请号：US11921873

申请日：2006-06-30

申请人： Marie-Therese Charreyre ,  Bernard Mandrand ,  Jose Manuel Gaspar Martinho ,  Paula Relogio ,  Jose Paulo Sequeira Farinha

发明人： Marie-Therese Charreyre ,  Bernard Mandrand ,  Jose Manuel Gaspar Martinho ,  Paula Relogio ,  Jose Paulo Sequeira Farinha

IPC分类号： C09K11/06

CPC分类号： C08F8/30 ,  C08F2/38 ,  C08F8/34 ,  C08F220/36 ,  C09B69/103 ,  C09B69/109

摘要： The invention relates to fluorescent polymers soluble in an aqueous solution carrying at least 5 fluorophores which are distributed on the polymer and which exhibit the following properties: the fluorophores are water-soluble, the fluorophores are not self-associated in water at a concentration of less than or equal to 10−4 mol/l, preferably at a concentration of less than or equal to 10−3 mol/l, the fluorophores which are free in the aqueous solution have a molar extinction coefficient of greater than 1000 M−1·cm−1, preferably greater than 5000 M−1·cm−1, the fluorophores which are free in the aqueous solution have a quantum yield of greater than 0.3, preferably greater than 0.6, said polymers having a fluorescence amplification factor of greater than or equal to 0.35 per kg/mol of polymer, preferably greater than 0.45 per kg/mol of polymer.

摘要翻译： 本发明涉及可溶于携带至少5个荧光团的水溶液中的荧光聚合物，其分布在聚合物上并且具有以下性质：荧光团是水溶性的，荧光团在水中不是以相对较少的浓度自相关 不大于或等于10-4mol / l，优选浓度小于或等于10-3mol / l，在水溶液中游离的荧光团具有大于1000M-1的摩尔吸光系数。 cm -1，优选大于5000M -1 cm -1，所述水溶液中游离的荧光团的量子产率大于0.3，优选大于0.6，所述聚合物的荧光放大系数大于或等于 等于每kg / mol聚合物0.35，优选大于0.45 / kg / mol聚合物。

公开(公告)号：US20070031452A1

公开(公告)日：2007-02-08

申请号：US11581030

申请日：2006-10-16

申请人： Herve Perron ,  Frederic Beseme ,  Frederic Bedin ,  Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel ,  Colette Jolivet-Reynaud ,  Bernard Mandrand ,  Jeremy Garson ,  Philip Tuke

发明人： Herve Perron ,  Frederic Beseme ,  Frederic Bedin ,  Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel ,  Colette Jolivet-Reynaud ,  Bernard Mandrand ,  Jeremy Garson ,  Philip Tuke

IPC分类号： C12P19/34 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N7/00 ,  C12N9/1241 ,  C12N2740/10022 ,  C12N2740/10051 ,  C12Q1/702

摘要： The invention provides viral material and nucleotide fragments associated with multiple sclerosis and/or rheumatoid arthritis for use in method of diagnosis, prophylaxis, and therapy.

摘要翻译： 本发明提供了用于诊断，预防和治疗方法的与多发性硬化和/或类风湿性关节炎有关的病毒物质和核苷酸片段。

公开(公告)号：US06866838B1

公开(公告)日：2005-03-15

申请号：US10129141

申请日：2000-11-06

申请人： Olivier Mondain-Monval ,  Abdelhamid Elaissari ,  Eric Bosc ,  Christian Pichot ,  Bernard Mandrand ,  Jérôme Bibette

发明人： Olivier Mondain-Monval ,  Abdelhamid Elaissari ,  Eric Bosc ,  Christian Pichot ,  Bernard Mandrand ,  Jérôme Bibette

IPC分类号： G01N33/545 ,  A61K9/51 ,  A61K31/7088 ,  A61K45/00 ,  A61K47/02 ,  A61K47/04 ,  A61K47/32 ,  A61K47/48 ,  A61K48/00 ,  A61P43/00 ,  C09D11/00 ,  G01N33/543 ,  A61B8/00 ,  A61K9/14 ,  B01J13/02

CPC分类号： G01N33/5434 ,  G01N2446/20 ,  G01N2446/64 ,  G01N2446/66 ,  Y10T428/2982 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2987

摘要： The invention concerns composite nanospheres having a diameter ranging between about 50 and 1000 nm plus or minus 5%, preferably between about 100 and 500 nm plus or minus 5% and advantageously between 100 and 200 nm plus or minus 5%, and comprising an essentially liquid core consisting of an organic phase and inorganic nanoparticles, distributed inside the organic phase, and a skin consisting of at least a hydrophilic polymer derived from the polymerisation of at least one water soluble monomer, in particular N-alkylacrylamide or a N—N-dialkylacrylamide; conjugates derived from said nanospheres; their preparation methods and their uses.

摘要翻译： 本发明涉及直径范围在约50和1000nm之间的复合纳米球，加上或减少5％，优选在约100和500nm之间加上或减少5％，并且有利地在100和200nm之间加上或减少5％，并且包括基本上 分布在有机相内部的有机相和无机纳米颗粒组成的液体芯，以及至少由至少一种水溶性单体，特别是N-烷基丙烯酰胺或NN-二烷基丙烯酰胺的聚合衍生的亲水性聚合物组成的皮肤; 源自所述纳米球的共轭物; 其制备方法及其用途。