公开(公告)号：US06673607B2

公开(公告)日：2004-01-06

申请号：US09859105

申请日：2001-05-16

申请人： Mehmet Toner ,  Ali Eroglu ,  Thomas Toth

发明人： Mehmet Toner ,  Ali Eroglu ,  Thomas Toth

IPC分类号： C12N500

CPC分类号： A01N1/0221 ,  Y10T436/108331

摘要： A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering the stored biological material from storage. Carbohydrate sugars such as trehalose, sucrose, fructose, dextran, and raffinose, may be used as bio-protective agents.

摘要翻译： 具有细胞膜的生物材料的保存方法包括用糖显微注射细胞; 准备储存的细胞; 储存生物材料; 并从储存中回收储存的生物材料。 碳水化合物糖如海藻糖，蔗糖，果糖，葡聚糖和棉子糖可用作生物保护剂。

公开(公告)号：US09976973B2

公开(公告)日：2018-05-22

申请号：US13884580

申请日：2011-11-09

申请人： Nicholas Watkins ,  Rashid Bashir ,  William Rodriguez ,  Xuanhong Cheng ,  Mehmet Toner ,  Grace Chen ,  Aaron Oppenheimer

发明人： Nicholas Watkins ,  Rashid Bashir ,  William Rodriguez ,  Xuanhong Cheng ,  Mehmet Toner ,  Grace Chen ,  Aaron Oppenheimer

IPC分类号： G01N15/10 ,  G01N27/02 ,  G01N15/12 ,  C12M1/34 ,  G01N33/569 ,  G01N15/14

CPC分类号： G01N27/02 ,  B01L2200/0652 ,  B01L2300/0877 ,  C12M41/36 ,  G01N15/12 ,  G01N15/1245 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1486

摘要： This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.

公开(公告)号：US08784012B2

公开(公告)日：2014-07-22

申请号：US13480862

申请日：2012-05-25

申请人： Mehmet Toner ,  Dino DiCarlo ,  Jon F. Edd ,  Daniel Irimia

发明人： Mehmet Toner ,  Dino DiCarlo ,  Jon F. Edd ,  Daniel Irimia

IPC分类号： B65G53/52

CPC分类号： B01L3/502776 ,  B01D21/0087 ,  B01D21/265 ,  B01D45/04 ,  B01D45/12 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0883 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/084 ,  B33Y80/00 ,  C12M23/16 ,  C12M41/36 ,  C12M47/04 ,  F16L41/02 ,  F17D1/00 ,  G01N15/0255 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/1081 ,  Y10T137/85938 ,  Y10T137/85978 ,  Y10T436/117497 ,  Y10T436/2575

摘要： Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

摘要翻译： 提供了各种系统，方法和装置，用于将悬浮在移动流体中的颗粒聚焦到一个或多个局部流线中。 该系统可以包括基底和设置在基底上的至少一个通道，其具有入口和出口。 该系统还可以包括在具有悬浮颗粒的层流中沿着通道移动的流体和驱动流体层流的泵送元件。 流体，通道和泵送元件可以被配置成使惯性力作用在颗粒上并将颗粒聚焦成一条或多条流线。

公开(公告)号：US20140030231A1

公开(公告)日：2014-01-30

申请号：US13695459

申请日：2011-05-04

申请人： Martin L. Yarmush ,  Mehmet Toner ,  Maria-Louisa Izamis ,  Timothy Antonie Berendsen ,  Robert Marius Bieganski ,  Osman Berk Usta ,  Basak Elif Uygun ,  Mustafa Korkut Uygun ,  Sinem Perk

发明人： Martin L. Yarmush ,  Mehmet Toner ,  Maria-Louisa Izamis ,  Timothy Antonie Berendsen ,  Robert Marius Bieganski ,  Osman Berk Usta ,  Basak Elif Uygun ,  Mustafa Korkut Uygun ,  Sinem Perk

IPC分类号： A01N1/02

CPC分类号： A01N1/0226 ,  G01N1/42 ,  G01N33/5091

摘要： The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

摘要翻译： 本发明一般涉及用于确定用于移植和其它医疗目的的器官的活力的方法和组合物。 本发明的一个方面涉及一种通过测量能量参数来确定器官的能量水平来评估器官活力的方法，该方法是通过确定存储的细胞能量（例如，ATP水平）和/或特定的能量消耗 生存时间段 能量参数可以与参考能量参数进行比较，作为对活细胞产量和器官存活力的高度准确和可靠的预测。 本发明的另一方面涉及保存或延长器官存活时间的方法，任何组合（i）器官的保留灌注以预防缺血性损伤，（ii）器官的化学代谢抑制，例如使用代谢抑制剂， （iii）物理或环境条件下的代谢抑制，例如零度非冻结储存。

公开(公告)号：US08043846B2

公开(公告)日：2011-10-25

申请号：US10560661

申请日：2004-06-09

申请人： Daniel Irimia ,  Mehmet Toner

发明人： Daniel Irimia ,  Mehmet Toner

IPC分类号： C12M1/34 ,  B01L3/00

CPC分类号： B01L3/502746 ,  B01F13/0066 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/046 ,  B01L2400/0487 ,  B01L2400/0688 ,  B33Y80/00 ,  C12M47/06 ,  Y10S366/02 ,  Y10S366/03 ,  Y10T436/118339

摘要： The invention features devices for mixing fluids, e.g., for lysing cells, and methods of use thereof. One device is based on the ability to control the flow of fluids, e.g., by contact angle and channel size. Fluids in this device can be divided to form segments of controlled volume, which are then brought together to initiate mixing. An exemplary use of the device is for the lysis of single cells. Another device is based on the ability to two mix two fluids in a channel and affinity capture of analytes. The devices can be integrated on the same chip with other devices, for example, for cell handling or analysis of DNA, mRNA, and proteins released from the lysis of a cell.

摘要翻译： 本发明的特征在于用于混合流体的装置，例如用于裂解细胞的装置及其使用方法。 一种装置基于例如通过接触角和通道尺寸来控制流体流动的能力。 该装置中的流体可以分成形成受控体积的区段，然后将其汇集在一起​​以开始混合。 该装置的示例性用途是用于单细胞的裂解。 另一种装置是基于两种混合通道中的两种流体和分析物的亲和性捕获的能力。 这些器件可以与其他器件集成在同一芯片上，例如用于细胞处理或分析从细胞裂解释放的DNA，mRNA和蛋白质。

公开(公告)号：US20100092393A1

公开(公告)日：2010-04-15

申请号：US12578303

申请日：2009-10-13

申请人： Ramin Haghgooie ,  Patrick Seamus Doyle ,  Mehmet Toner ,  Daniel Colin Pregibon

发明人： Ramin Haghgooie ,  Patrick Seamus Doyle ,  Mehmet Toner ,  Daniel Colin Pregibon

IPC分类号： A61K9/50 ,  C08J3/28 ,  C09K11/02 ,  B32B5/16 ,  C09D7/00 ,  A61K49/00 ,  G01N33/566

CPC分类号： C08J3/075 ,  A61K47/68 ,  A61K47/6921 ,  C08J2371/02 ,  C08L2205/14 ,  C09D7/20 ,  C09D7/70 ,  Y10T428/2982

摘要： Techniques are provided to produce and use non-spherical colloidal particles with independently tuned size, shape, flexibility, and chemical properties. A pre-polymer mixture for forming hydrogel particles includes a percentage of PEGDA selected to impart a target stiffness to the particles and includes, a percentage of acrylic acid selected to impart an independent target chemical function to the particles. The mixture also includes a percentage of photo-initiator to polymerize PEGDA upon exposure to a light source to impart an independently selected target size or shape or both to the particles.

摘要翻译： 提供技术来生产和使用具有独立调整的尺寸，形状，柔性和化学性质的非球形胶体颗粒。 用于形成水凝胶颗粒的预聚物混合物包括选择用于赋予颗粒目标硬度的百分比的PEGDA，并且包括一定百分比的丙烯酸，其被选择以赋予颗粒独立的目标化学功能。 该混合物还包括一定百分比的光引发剂，以在暴露于光源时聚合PEGDA以赋予颗粒独立选择的靶尺寸或形状或两者。

公开(公告)号：US20100021984A1

公开(公告)日：2010-01-28

申请号：US12472346

申请日：2009-05-26

申请人： Jon F. Edd ,  Mehmet Toner ,  Dino Dicarlo ,  Daniel Irimia

发明人： Jon F. Edd ,  Mehmet Toner ,  Dino Dicarlo ,  Daniel Irimia

IPC分类号： C12N11/00

CPC分类号： C12N11/04

摘要： Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.

摘要翻译： 公开了用于在液滴内包封颗粒的微流体装置和方法。 新的方法和装置形成1-100皮升大小的单分散液滴，其包含单个细胞的颗粒，包封在单个液滴中。 通过使含有颗粒的流体通过高纵横比的微通道将粒子封装在流体的液滴中，以使颗粒在流体中排列，然后将流体形成液滴。 所得到的具有单个颗粒（例如，电池）的液滴的部分高于由泊松统计预测的单颗粒液滴的相应部分。

公开(公告)号：US20090221073A1

公开(公告)日：2009-09-03

申请号：US12089440

申请日：2006-10-10

申请人： Mehmet Toner ,  Daniel Irimia

发明人： Mehmet Toner ,  Daniel Irimia

IPC分类号： C12N5/06 ,  C12M1/00

CPC分类号： F16K99/0059 ,  B01L3/502738 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/0677 ,  C12M47/06 ,  F16K99/0001 ,  F16K99/0005 ,  F16K99/0034 ,  F16K2099/0074 ,  F16K2099/0084

摘要： Devices for fluid control and biological particle manipulation (e.g., cell enrichment and blood sampling) are disclosed. The devices a based on the ability to control the flow of fluids through the use of microfluidic valves. The valves are characterized, for example, by microstructures disposed on a mobile diaphragm.

摘要翻译： 公开了用于流体控制和生物粒子操纵（例如细胞富集和血液取样）的装置。 装置a基于通过使用微流体阀控制流体流动的能力。 阀的特征在于例如通过设置在移动隔膜上的微结构。

公开(公告)号：US20070026414A1

公开(公告)日：2007-02-01

申请号：US11323945

申请日：2005-12-29

申请人： Martin Fuchs ,  Mehmet Toner

发明人： Martin Fuchs ,  Mehmet Toner

IPC分类号： C12Q1/68 ,  G01N33/567 ,  C12M1/34

CPC分类号： C12Q1/6886 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q2600/16 ,  C12Q2600/178 ,  G01N2800/52

摘要： The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.

公开(公告)号：US06562616B1

公开(公告)日：2003-05-13

申请号：US09599297

申请日：2000-06-21

申请人： Mehmet Toner ,  Martin L. Yarmush ,  Ulysses J. Balis ,  Arno W. Tilles

发明人： Mehmet Toner ,  Martin L. Yarmush ,  Ulysses J. Balis ,  Arno W. Tilles

IPC分类号： C12M304

CPC分类号： A61M1/3633 ,  A61F2/022 ,  A61M2210/1071 ,  C12M23/24 ,  C12M23/34 ,  C12M29/04 ,  C12M29/10 ,  C12M41/00

摘要： The invention relates to new flow-through cell culturing devices that include plates arranged in parallel and spaced to control the flow patterns and velocity of liquid medium flowing between the plates. The devices can also include gas-permeable, liquid impermeable membranes arranged between the plates. The devices can be used to culture cells, such as hepatocytes, at high levels of mass transport of nutrients, oxygen, and waste products, yet low levels of shear stress for extended periods of time and with high levels of cell function, e.g., in organ assist systems. The invention also includes new culturing plates for use in the devices, and methods of manufacturing these plates.

摘要翻译： 本发明涉及新的流通细胞培养装置，其包括平行布置并间隔开的板，以控制在板之间流动的液体介质的流动模式和速度。 装置还可以包括布置在板之间的透气的液体不可渗透膜。 这些装置可以用于培养细胞，如肝细胞，在营养物质，氧气和废物产品的高质量运输水平下，延长的时间段和高水平的细胞功能的低水平的剪切应力，例如， 器官辅助系统。 本发明还包括用于装置的新培养板以及制造这些板的方法。