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    DIAGNOSTIC PROBE DETECTION SYSTEM
    71.
    发明申请
    DIAGNOSTIC PROBE DETECTION SYSTEM 有权
    诊断探针检测系​​统

    公开(公告)号：US20130330725A1

    公开(公告)日：2013-12-12

    申请号：US13967249

    申请日：2013-08-14

    申请人： ONE LAMBDA

    发明人： Katsuyuki Saito ,  Jar-How Lee ,  Lindley Blair

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2535/131 ,  C12Q2525/197

    摘要： The invention provides methods for detecting target nucleic acid sequences with diagnostic probes including first and second probe regions that are substantially complementary to first and second target regions respectively on a target nucleic acid strand wherein the first probe region is located 5′ to the second probe region. The first probe region is substantially complementary to the first target region, on the target nucleic acid strand, which also includes a second target region, wherein when said first target region is contiguous with the second target region on the target nucleic acid strand, then the first and second probe regions on the diagnostic probe are separated by a spacer region of nucleic acid.

    摘要翻译： 本发明提供了用诊断探针检测靶核酸序列的方法，所述诊断探针包括分别在靶核酸链上与第一和第二靶区基本上互补的第一和第二探针区，其中第一探针区位于第二探针区5' 。 第一探针区域与目标核酸链上的第一靶区基本上互补，靶核酸链还包括第二靶区，其中当所述第一靶区与靶核酸链上的第二靶区连续时，则 诊断探针上的第一和第二探针区域被核酸的间隔区分开。

    SPECIFIC BIOMARKER FOR IDENTIFICATON OF EXPOSURE TO PROPIONALDEHYDE AND THE METHOD OF IDENTIFICATION USING THE SAME
    72.
    发明申请
    SPECIFIC BIOMARKER FOR IDENTIFICATON OF EXPOSURE TO PROPIONALDEHYDE AND THE METHOD OF IDENTIFICATION USING THE SAME 审中-公开
    特异性生物标志物，以及使用该化合物的鉴定方法

    公开(公告)号：US20130281311A1

    公开(公告)日：2013-10-24

    申请号：US13689042

    申请日：2012-11-29

    申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    发明人： Jae Chun Ryu ,  Mee Song ,  Ji-seong Yoon ,  Hyo Sun Lee ,  Wooin Ryu ,  Chan Young Shin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/142 ,  C12Q2600/158

    摘要： The present invention relates to a biomarker for the identification of specific exposure to propionaldehyde which is one of volatile organic compounds exposed in the environment, and a method for the identification of specific exposure to propionaldehyde using the same, precisely a biomarker which is up-regulated or down-regulated specifically by propionaldehyde and a method for the identification of specific exposure to propionaldehyde using the biomarker. The biomarker of the present invention is the reacted genes selected by using DNA microarray chip, which can be effectively used for the monitoring and evaluation of propionaldehyde contamination in the environment samples and at the same time as a tool for the investigation of the toxic mechanism induced specifically by propionaldehyde.

    摘要翻译： 本发明涉及用于鉴定作为在环境中暴露的挥发性有机化合物之一的丙醛的特异性暴露的生物标志物，以及使用该方法鉴定特异性暴露于丙醛的方法，精确地鉴定了上调的生物标志物 或通过丙醛特异性下调，以及使用生物标志物鉴定特异性暴露于丙醛的方法。 本发明的生物标志物是通过使用DNA微阵列芯片选择的反应基因，其可以有效地用于环境样品中丙醛污染的监测和评估，同时作为用于研究诱导的毒性机制的工具 特别是丙醛。

    Snap-back primers and detectable hairpin structures
    80.
    发明授权
    Snap-back primers and detectable hairpin structures 有权
    快速反向引物和可检测的发夹结构

    公开(公告)号：US08445238B2

    公开(公告)日：2013-05-21

    申请号：US11866663

    申请日：2007-10-03

    申请人： Jeff G. Hall ,  Andrew A. Lukowiak ,  Patrick Peterson

    发明人： Jeff G. Hall ,  Andrew A. Lukowiak ,  Patrick Peterson

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6858 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/301 ,  C12Q2561/109

    摘要： The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3′ hairpin structures, 5′ hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5′ or 3′ ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.

    摘要翻译： 本发明提供了包含用于形成3'发夹结构，5'发夹结构和双发夹结构的快速反应引物的方法，组合物和试剂盒。 发夹结构可用于检测目标序列（例如，例如小RNA靶序列），用于检测靶序列中的多态性（例如，位于靶序列的5'或3'末端附近的多态性）或其他 核酸表征方法。 在某些实施方案中，发夹结构形成侵入性切割结构（例如，与探针或上游寡核苷酸组合），其可被结构特异性酶切割以检测特定核苷酸或核苷酸序列的存在或不存在。