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92 条记录 (耗时 0.06 秒)

    Metalloproteinase peptides
    84.
    发明授权
    Metalloproteinase peptides 失效
    金属蛋白酶

    公开(公告)号：US5698671A

    公开(公告)日：1997-12-16

    申请号：US284721

    申请日：1994-08-02

    申请人： William G. Stetler-Stevenson ,  Lance A. Liotta ,  Henry C. Krutzsch

    发明人： William G. Stetler-Stevenson ,  Lance A. Liotta ,  Henry C. Krutzsch

    IPC分类号： A61K38/00 ,  C07K14/81 ,  C07K7/00

    CPC分类号： C07K14/8146 ,  A61K38/00

    摘要： Inappropriate degradation of extracellular matrix molecules by metalloproteinases plays an important role in a wide variety of pathologic conditions including neoplasia and arthritis. The present invention is an isolated protein of approximately 23,000 daltons in size which binds to metalloproteinases with high affinity, can be purified using affinity chromatography on solid phase metalloproteinases, and is potentially useful for therapy of pathologic conditions involving the inappropriate production of metalloproteinases. This protein is characterized by the presence of the following amino acid sequences: CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNPIYGNNI KDIEFIYTAPSEAVCGVELDVEGK KRHITLCDFIVPWDTLSTTQKKSLNHRYQQGCEECKITRCPMIPCYISSPDECLWTDTVV KFFACIKRHITLCDFIVPWSQIADXLSS With the positions of the cysteine residues and associated disulfide bridges required for biologic activity.

    摘要翻译： 金属蛋白酶对细胞外基质分子的不适当降解在包括肿瘤形成和关节炎在内的多种病理状况中起重要作用。 本发明是大约23,000道尔顿的分离的蛋白质，其以高亲和力结合金属蛋白酶，可以使用固相金属蛋白酶上的亲和层析纯化，并且可能用于治疗涉及金属蛋白酶不适当的病理状况。 该蛋白的特征在于存在以下氨基酸序列：CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNPIYGNNI KDIEFIYTAPSEAVCGVELDVEGK KRHITLCDFIVPWDTLSTTQKKSLNHRYQQGCEECKITRCPMIPCYISSPDECLWTDTVV KFFACIKRHITLCDFIVPWSQIADXLSS具有生物活性所需的半胱氨酸残基和相关二硫键的位置。

    Matrix metalloproteinase peptides: role in diagnosis and therapy
    85.
    发明授权
    Matrix metalloproteinase peptides: role in diagnosis and therapy 失效
    基质金属蛋白酶肽：在诊断和治疗中的作用

    公开(公告)号：US5585356A

    公开(公告)日：1996-12-17

    申请号：US289825

    申请日：1994-08-12

    申请人： Lance A. Liotta ,  William G. Stetler-Stevenson ,  Henry C. Krutsch

    发明人： Lance A. Liotta ,  William G. Stetler-Stevenson ,  Henry C. Krutsch

    IPC分类号： G01N33/53 ,  A61K9/08 ,  A61K38/55 ,  A61P19/02 ,  A61P35/00 ,  A61P43/00 ,  C07H21/04 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/81 ,  C07K16/00 ,  C07K16/38 ,  C07K17/00 ,  C12N5/10 ,  C12N9/99 ,  C12N15/09 ,  G01N33/573 ,  A61K38/00

    CPC分类号： C07K14/8146 ,  G01N33/573 ,  Y10S530/806 ,  Y10S530/81

    摘要： A family of metalloproteinases exist which cleave extracellular matrix molecules. These metalloproteinases are secreted in a latent inactive form and require activation in order to specifically cleave the preferred substrate. A series of peptides have been prepared based on the complete sequence analysis of type IV procollagenase. Peptide inhibitors were synthesized which correspond to cysteine repeat regions and histidine containing regions; the mechanism of action of these peptides involves inhibition of binding of the enzyme to the substrate. Peptide inhibitors were synthesized which correspond to the peptide cleaved off during activation, and constitute a novel class of metalloproteinase inhibitors. These inhibitors are members of a series of peptides which contain the core amino acid sequence RKPRC or analogs thereof. The cysteine residue is required for activity. Affinity purified antibodies directed against specific peptides can be used to a) detect any general metalloproteinase enzyme with the sequence in part VAAHE or PRCGNPD, and distinguish it from other known members of the metalloproteinase family, b) block functional domains resulting in the inhibition of enzyme activity, and c) distinguish latent from activated forms of the enzyme.

    摘要翻译： 存在切割细胞外基质分子的金属蛋白酶家族。 这些金属蛋白酶以潜伏活性形式分泌并需要活化以特异性切割优选的底物。 已经基于IV型原胶原酶的完整序列分析制备了一系列肽。 合成肽抑制剂，其对应于半胱氨酸重复区域和组氨酸区域; 这些肽的作用机制涉及抑制酶与底物的结合。 合成肽抑制剂，其对应于在活化期间切除的肽，并构成新型的金属蛋白酶抑制剂。 这些抑制剂是含有核心氨基酸序列RKPRC或其类似物的一系列肽的成员。 活性需要半胱氨酸残基。 针对特异性肽的亲和纯化抗体可用于a）用部分VAAHE或PRCGNPD中的序列检测任何一般金属蛋白酶，并将其与其他已知的金属蛋白酶家族成员区分开，b）阻断功能结构域，导致酶的抑制 活性，和c）区分潜在和酶的活化形式。

    Layered immunoassay using antibodies bound to transport particles to determine the presence of an antigen
    87.
    发明授权
    Layered immunoassay using antibodies bound to transport particles to determine the presence of an antigen 失效
    使用结合传输颗粒以确定抗原存在的抗体进行分层免疫测定

    公开(公告)号：US4837145A

    公开(公告)日：1989-06-06

    申请号：US871857

    申请日：1986-06-09

    申请人： Lance A. Liotta

    发明人： Lance A. Liotta

    IPC分类号： G01N33/543 ,  G01N33/537 ,  G01N33/558

    CPC分类号： G01N33/537 ,  G01N33/558 ,  Y10S436/807 ,  Y10S436/819 ,  Y10S436/824 ,  Y10S436/828

    摘要： A device is provided for determining the presence of an antigen which comprises a trapping zone, which contains material capable of capturing free flowing enzyme linked antibodies, but not antibodies bound to a transport particle which flows freely through the trapping zone into the substrate zone, and a substrate zone which contains material capable of reacting with enzyme-linked antibodies to produce a reaction which indicates the presence of antibodies. A method of determining the presence of an antigen is provided wherein a sample is mixed with two classes of antibodies which are specific for the antigen being tested for, but which react with different antigen domains, wherein the mixture consists of class one antibodies bound to a transport particle which flows freely through the trapping zone and class two enzyme-linked antibodies which are incapable, unless bound to the transport particles, of flowing freely through the trapping zone. In the presence of the antigen being tested for, both classes of antibodies bind to the antigen and flow through the trapping zone into the substrate zone, wherein a reaction takes place to indicate the presence of the antigen.

    摘要翻译： 提供了一种用于确定抗原存在的装置，其包括捕获区，其包含能够捕获自由流动的酶连接的抗体的物质，但不包含结合到通过捕获区自由流动进入基质区的转运颗粒的抗体，以及 底物区域，其含有能够与酶联抗体反应以产生表示抗体存在的反应的材料。 提供了确定抗原存在的方法，其中将样品与针对所测试的抗原特异性但与不同抗原结构域反应的两类抗体混合，其中该混合物由与第一类抗体结合的一类抗体组成 通过捕获区自由流动的运输颗粒和不能与运输颗粒结合的无法流动的两类酶联抗体自由流过捕获区。 在所测试的抗原存在下，两种类型的抗体结合抗原并流过捕获区进入底物区，其中发生反应以指示抗原的存在。

    Enzyme immunoassay with two-zoned device having bound antigens
    90.
    发明授权
    Enzyme immunoassay with two-zoned device having bound antigens 失效
    具有结合抗原的双区装置的酶免疫测定

    公开(公告)号：US4446232A

    公开(公告)日：1984-05-01

    申请号：US310801

    申请日：1981-10-13

    申请人： Lance A. Liotta

    发明人： Lance A. Liotta

    IPC分类号： G01N33/52 ,  G01N33/53 ,  G01N33/543 ,  G01N33/558 ,  G01N33/54 ,  G01N33/58 ,  G01N33/76

    CPC分类号： G01N33/558 ,  G01N33/525 ,  G01N33/54386 ,  Y10S435/805 ,  Y10S435/97 ,  Y10S436/81 ,  Y10S436/818 ,  Y10S436/82

    摘要： A device for determining the presence of antigens which comprises a first zone containing antigens and enzyme-linked antibodies which are capable of immunologically reacting with said antigens, said antibodies being positioned in said first zone such that they will be removed from said first zone when reacted with antigens passing through said first zone but not removed from said first zone in the absence of such antigens, and a second zone containing material capable of reacting with said enzyme-linked antibodies to produce a color forming reaction which indicates the presence of said antibodies.

    摘要翻译： 一种用于确定抗原存在的装置，其包括含有抗原的第一区和能够与所述抗原免疫反应的酶联抗体，所述抗体位于所述第一区中，使得当反应时将其从所述第一区移除 其中抗原通过所述第一区，但在不存在这样的抗原的情况下不从所述第一区移除;以及第二区含有能够与所述酶连接的抗体反应的物质，以产生指示所述抗体存在的成色反应。