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公开(公告)号：US20080044920A1
公开(公告)日：2008-02-21
申请号：US11675980
申请日：2007-02-16
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G06F19/20 , G06F19/22 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, i.e., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常被布置在检测块中，每个区块能够区分给定标记，即杂合子或两个纯合子中的任一个的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析
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公开(公告)号：US20060110752A1
公开(公告)日：2006-05-25
申请号：US11247053
申请日：2005-10-11
IPC分类号： C12Q1/68
CPC分类号： C07H21/04 , B01J2219/00432 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C12Q1/6827 , C12Q1/6874 , C40B40/06 , C40B60/14 , G16B25/00 , G16B30/00 , Y10T436/143333 , C12Q2565/501 , C12Q2525/179 , C12Q2525/204 , C12Q2525/185 , C12Q2535/131
摘要： The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e g, the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits
摘要翻译： 本发明通常提供用于分析多态性或双重标记的快速有效的方法和用于进行这些分析的阵列。 通常，本发明的方法使用与对应于个体的标记序列的靶核酸互补的寡核苷酸探针阵列。 探针通常设置在检测块中，每个区段能够区分给定标记（例如杂合子或两个纯合子中的任一个）的三种基因型。 该方法允许对甚至复杂的多基因性状的遗传连锁的快速，自动化分析
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3.发明申请Capturing sequences adjacent to type-IIS restriction sites for genomic library mapping 有权捕获与基因组文库映射的类型-Ill限制位点相邻的序列公开(公告)号：US20050026212A1
公开(公告)日：2005-02-03
申请号：US10942479
申请日：2004-09-16
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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4.发明申请Capturing sequences adjacent to type-IIS restriction sites for genomic library mapping 审中-公开捕获与基因组文库映射的类型-Ill限制位点相邻的序列公开(公告)号：US20060223097A1
公开(公告)日：2006-10-05
申请号：US11390803
申请日：2006-06-12
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic napping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特异性寡核苷酸序列。 所得序列可用作用于遗传睡眠，筛选和操作的有效标记物。
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公开(公告)号：US20060263807A1
公开(公告)日：2006-11-23
申请号：US11388199
申请日：2006-03-24
申请人： Robert Lipshutz , Stephen Fodor
发明人： Robert Lipshutz , Stephen Fodor
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00659 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B60/14 , Y02A90/24 , C12Q2565/501 , C12Q2565/515 , C12Q2525/179
摘要： The invention provides methods of using probe arrays For polymorphism identification and profiling. Such methods entail constructing a first array of probes that span and are complementary to one or more known DNA sequences. This array is hybridized with nucleic acid samples from different individuals to identify a collection of polymorphisms. A second array is then constructed to determine a polymorphic profile of an individual at the collection of polymorphic sites. The polymorphic profile is useful for, e.g., genetic mapping, epidemiology, diagnosis and forensics.
摘要翻译： 本发明提供使用探针阵列的方法用于多态性鉴定和分析。 这样的方法需要构建跨越并与一个或多个已知DNA序列互补的探针的第一阵列。 该阵列与来自不同个体的核酸样品杂交以鉴定多态性的集合。 然后构建第二阵列以确定在多态位点的收集处的个体的多态性轮廓。 多态性谱可用于例如遗传图谱，流行病学，诊断和取证。
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公开(公告)号：US20060246490A1
公开(公告)日：2006-11-02
申请号：US11396958
申请日：2006-04-04
申请人： Rolfe Anderson , Robert Lipshutz , Richard Rava , Stephen Fodor
发明人： Rolfe Anderson , Robert Lipshutz , Richard Rava , Stephen Fodor
CPC分类号： B01L7/52 , B01F11/0071 , B01F11/0266 , B01F13/005 , B01F13/0059 , B01F13/0062 , B01F13/0071 , B01F13/0222 , B01F13/08 , B01F13/0809 , B01F2215/0037 , B01F2215/0073 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502753 , B01L3/502784 , B01L7/525 , B01L2200/0621 , B01L2200/0673 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0861 , B01L2300/087 , B01L2300/0883 , B01L2300/16 , B01L2300/1827 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/086
摘要： The present invention provides a miniaturized integrated nucleic acid diagnostic device and system.
摘要翻译： 本发明提供了一种小型化的综合核酸诊断装置和系统。
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公开(公告)号：US20060229824A1
公开(公告)日：2006-10-12
申请号：US11367800
申请日：2006-03-03
申请人： Maureen Cronin , Charles Miyada , Earl Hubbell , Mark Chee , Stephen Fodor , Xiaohua Huang , Robert Lipshutz , Peter Lobban , MacDonald Morris , Edward Sheldon
发明人： Maureen Cronin , Charles Miyada , Earl Hubbell , Mark Chee , Stephen Fodor , Xiaohua Huang , Robert Lipshutz , Peter Lobban , MacDonald Morris , Edward Sheldon
IPC分类号： G06F19/00
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/156 , C12Q2600/172 , C40B40/06 , C40B60/14
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.
摘要翻译： 本发明提供了固定化探针的阵列，以及采用该阵列的方法，用于检测生物转化基因如细胞色素P450中的突变。 例如，一个这样的阵列包括四个探针组。 第一探针组包含多个探针，每个探针包含与来自生物转化基因的参考序列的亚序列完全互补的至少三个核苷酸的片段，该片段包含与参考文献中的相应核苷酸互补的至少一个询问位置 序列。 第二，第三和第四探针组各自包括在第一探针组中的每个探针的相应探针。 第二，第三和第四探针组中的探针与包含来自第一探针组的相应探针或包含至少一个询问位置的至少三个核苷酸的亚序列的序列相同，除了至少一个询问 位置由来自四个探针组的四个相应探针中的每一个中的不同核苷酸占据。
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公开(公告)号：US20050118633A1
公开(公告)日：2005-06-02
申请号：US10984127
申请日：2004-11-08
申请人： Robert Lipshutz , MacDonald Morris , Mark Chee , Thomas Gingeras
发明人： Robert Lipshutz , MacDonald Morris , Mark Chee , Thomas Gingeras
CPC分类号： C12Q1/6806 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q1/6809 , C12Q1/6837 , C40B40/06 , C12Q2565/107 , C12Q2525/155
摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided.
摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许同时从样品中选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。
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公开(公告)号：US20050196792A1
公开(公告)日：2005-09-08
申请号：US11058566
申请日：2005-02-14
申请人： Stephen Fodor , Robert Lipshutz , Shivani Nautiyal
发明人： Stephen Fodor , Robert Lipshutz , Shivani Nautiyal
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6844 , C12Q1/686 , C12Q2531/113 , C12Q2521/331 , C12Q2565/501 , C12Q2533/101 , C12Q2525/179 , C12Q2521/125 , C12Q2531/119
摘要： Methods for amplifying a nucleic acid sample while preserving the methylation status of cytosines are disclosed. In some aspects the amplified methylated sample is modified by methylation sensitive modification and analyzed by hybridization to an array to identify cytosines that were methylated in the starting material and cytosines that were not methylated in the starting material. Methods for detecting methylation status are also disclosed. In one embodiment a DNA methyltransferase activity is included in the amplification reaction and this activity methylates the newly synthesized DNA using the methylated genomic template strand as a guide.
摘要翻译： 公开了扩增核酸样品同时保持胞嘧啶甲基化状态的方法。 在一些方面，通过甲基化敏感性修饰来修饰扩增的甲基化样品，并通过与阵列杂交来分析，以鉴定起始原料中甲基化的胞嘧啶和起始材料中未被甲基化的胞嘧啶。 还公开了检测甲基化状态的方法。 在一个实施方案中，DNA甲基转移酶活性包括在扩增反应中，并且该活性使用甲基化基因组模板链作为指导对新合成的DNA进行甲基化。
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公开(公告)号：US20060204991A1
公开(公告)日：2006-09-14
申请号：US11360146
申请日：2006-02-22
申请人： Robert Lipshutz , MacDonald Morris , Mark Chee , Thomas Gingeras
发明人： Robert Lipshutz , MacDonald Morris , Mark Chee , Thomas Gingeras
CPC分类号： C12Q1/6806 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q1/6809 , C12Q1/6837 , C40B40/06 , C12Q2565/107 , C12Q2525/155
摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample Methods of producing such affinity matrices are also provided In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequence b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids, and c) attaching the pool of nucleic acids to a solid support
摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许从样品中同时选择和除去大量预选择的核酸。还提供了产生这种亲和基质的方法一般来说，该方法涉及 步骤a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中，所述寡核苷酸的密度较大 每1cm 2超过约60个不同的寡核苷酸，并且所有所述不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列b）扩增所述多重寡核苷酸以提供 一组扩增的核酸，以及c）附着核酸池 酸固体支持物
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