公开(公告)号：US20070045117A1

公开(公告)日：2007-03-01

申请号：US11585618

申请日：2006-10-23

申请人： Vamsee Pamula ,  Michael Pollack ,  Hong Ren ,  Richard Fair

发明人： Vamsee Pamula ,  Michael Pollack ,  Hong Ren ,  Richard Fair

IPC分类号： C25B9/00 ,  G01N27/00

CPC分类号： B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01J2219/00479 ,  B01J2219/00653 ,  B01L3/502792 ,  B01L2300/089 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  C40B60/14 ,  G01N15/14 ,  G01N2035/1046 ,  G02B26/005 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Apparatuses for mixing droplets, such as a binary mixing apparatus, are provided. The binary mixing apparatus includes an array of electrodes and a conducting element positioned in relation to at least one of the electrodes to enable a droplet placed in electrical communication with the at least one electrode to electrically communicate with the conducting element. The binary mixing apparatus additionally includes an electrode selector for sequentially biasing one or more selected electrodes of the array to move a droplet disposed on the array into contact with another droplet. The apparatus further includes a first droplet supply area communicating with the array and a second droplet supply area communicating with the array.

摘要翻译： 提供了用于混合液滴的装置，例如二元混合装置。 二进制混合装置包括电极阵列和相对于至少一个电极定位的导电元件，使得与至少一个电极电连通的液滴能够与导电元件电连通。 二进制混合装置还包括用于顺序地偏置阵列的一个或多个所选择的电极的电极选择器，以移动设置在阵列上的液滴与另一液滴接触。 该装置还包括与阵列连通的第一液滴供应区域和与阵列连通的第二液滴供应区域。

公开(公告)号：US20060054503A1

公开(公告)日：2006-03-16

申请号：US11077569

申请日：2005-03-10

申请人： Vamsee Pamula ,  Michael Pollack ,  Philip Paik ,  Hong Ren ,  Richard Fair

发明人： Vamsee Pamula ,  Michael Pollack ,  Philip Paik ,  Hong Ren ,  Richard Fair

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

摘要： Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

公开(公告)号：US20070217956A1

公开(公告)日：2007-09-20

申请号：US11753318

申请日：2007-05-24

申请人： Vamsee Pamula ,  Michael Pollack ,  Richard Fair

发明人： Vamsee Pamula ,  Michael Pollack ,  Richard Fair

IPC分类号： B01L3/02 ,  B32B27/04 ,  B32B27/12 ,  B32B5/02

CPC分类号： G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

摘要： Methods for amplifying nucleic acid, such as DNA or RNA, on a printed circuit board (PCB) substrate are disclosed. The amplification can be achieved through a variety of methods such as thermocycling or isothermally. The printed circuit board substrate can comprise an array of electrodes for transporting droplets and can be part of a sandwich structure including a top plate parallel to the printed circuit board substrate.

摘要翻译： 公开了在印刷电路板（PCB）衬底上放大核酸如DNA或RNA的方法。 扩增可以通过各种方法如热循环或等温进行。 印刷电路板基板可以包括用于输送液滴的电极阵列，并且可以是包括平行于印刷电路板基板的顶板的夹层结构的一部分。

公开(公告)号：US20060194331A1

公开(公告)日：2006-08-31

申请号：US11343284

申请日：2006-01-30

申请人： Vamsee Pamula ,  Michael Pollack ,  Richard Fair

发明人： Vamsee Pamula ,  Michael Pollack ,  Richard Fair

IPC分类号： G05D19/00

CPC分类号： G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

摘要： Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.

公开(公告)号：US20070037294A1

公开(公告)日：2007-02-15

申请号：US11585491

申请日：2006-10-23

申请人： Vamsee Pamula ,  Michael Pollack ,  Richard Fair

发明人： Vamsee Pamula ,  Michael Pollack ,  Richard Fair

IPC分类号： G01N1/10

CPC分类号： B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01J2219/00479 ,  B01J2219/00653 ,  B01L3/502792 ,  B01L2300/089 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  C40B60/14 ,  G01N15/14 ,  G01N2035/1046 ,  G02B26/005 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Methods for performing microfluidic sampling are provided. The method includes providing a substrate including an arrangement of first, second and third electrodes, wherein the second electrode is interposed between the first and third electrodes. The method additionally includes causing a fluid input to continuously flow to the first electrode and biasing the first, second and third electrodes to a first voltage to cause a portion of the fluid input to spread across the second and third electrodes. The method further includes biasing the second electrode to a second voltage different from the first voltage to form a droplet on the third electrode, the droplet being separate from the fluid input.

摘要翻译： 提供了执行微流体取样的方法。 该方法包括提供包括第一，第二和第三电极的布置的基板，其中第二电极介于第一和第三电极之间。 该方法还包括使流体输入连续地流动到第一电极并且将第一，第二和第三电极偏压到第一电压以使流体输入的一部分扩展穿过第二和第三电极。 该方法还包括将第二电极偏压到不同于第一电压的第二电压，以在第三电极上形成液滴，液滴与流体输入分离。

公开(公告)号：US20080105549A1

公开(公告)日：2008-05-08

申请号：US11936289

申请日：2007-11-07

申请人： Vamsee Pamela ,  Michael Pollack ,  Philip Paik ,  Hong Ren ,  Richard Fair

发明人： Vamsee Pamela ,  Michael Pollack ,  Philip Paik ,  Hong Ren ,  Richard Fair

IPC分类号： G01N27/26

CPC分类号： B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01J2219/00479 ,  B01J2219/00653 ,  B01L3/502792 ,  B01L2300/089 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  C40B60/14 ,  G01N15/14 ,  G01N2035/1046 ,  G02B26/005 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

摘要： Methods for sampling a liquid flow are provided. The method includes supplying a continuous liquid flow from a continuous flow fluid input source to a surface along a first flow path. The method additionally includes sampling the continuous liquid flow by performing an electric field-based technique to split off a sample droplet from a portion of the liquid flow, whereby the sample droplet is distinct from the liquid flow and controllable independently of the liquid flow, wherein the electric field-based technique is performed by providing a set of electrodes on the surface and selectively biasing the set of electrodes, whereby the sample droplet is formed on one of the set of electrodes.

摘要翻译： 提供了液体取样方法。 该方法包括将连续的液体流从连续流体流体输入源提供到沿着第一流动路径的表面。 该方法另外包括通过执行基于电场的技术对液体流的一部分分离样品液滴来对连续液体流进行采样，由此样品液滴与液体流不同，并且可独立于液体流动而控制，其中 通过在表面上设置一组电极并选择性地偏置该组电极来执行基于电场的技术，由此在一组电极中形成样品液滴。

公开(公告)号：US20080044893A1

公开(公告)日：2008-02-21

申请号：US11839774

申请日：2007-08-16

申请人： Michael Pollack ,  Vamsee Pamula ,  Vijay Srinivasan

发明人： Michael Pollack ,  Vamsee Pamula ,  Vijay Srinivasan

IPC分类号： C12M1/40 ,  B01L3/00 ,  G01N33/53

CPC分类号： B01L3/502784 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00495 ,  B01J2219/00587 ,  B01J2219/00725 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07K1/1136 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/2575

摘要： Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.

摘要翻译： 提供多孔液滴致动器，系统和方法。 根据一个实施例，提供了一种衬底，其包括：（a）用于引入一种或多种试剂和/或样品的一个或多个输入端口; （b）一系列加工井; 和（c）液滴传输路径的网络，其包括提供从每个输入端口到所述一个或多个处理井中的每一个的直接或间接液滴传输的路径。 还提供了与其相关的不同液滴致动器和系统。

公开(公告)号：US20080053205A1

公开(公告)日：2008-03-06

申请号：US11639566

申请日：2006-12-15

申请人： Michael Pollack ,  Vamsee Pamula ,  Vijay Srinivasan

发明人： Michael Pollack ,  Vamsee Pamula ,  Vijay Srinivasan

IPC分类号： G01N15/06 ,  B01J19/08

CPC分类号： G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  C12Q1/6806 ,  G01N15/1031 ,  G01N27/44717 ,  G01N27/44721 ,  G01N33/543 ,  G01N35/10 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.

摘要翻译： 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

公开(公告)号：US20080006535A1

公开(公告)日：2008-01-10

申请号：US11838388

申请日：2007-08-14

申请人： Philip Paik ,  Michael Pollack ,  Ryan Sturmer ,  Gregory Smith ,  Keith Brafford ,  Vamsee Pamula

发明人： Philip Paik ,  Michael Pollack ,  Ryan Sturmer ,  Gregory Smith ,  Keith Brafford ,  Vamsee Pamula

IPC分类号： G01N27/00

CPC分类号： B01L3/502792 ,  B01L2200/143 ,  B01L2300/027 ,  B01L2300/0816 ,  B01L2400/0427

摘要： Systems for controlling a droplet microactuator are provided. According to one embodiment, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, and a display device displaying a user interface electronically coupled to the controller, wherein the system is programmed and configured to permit a user to effect a droplet manipulation by interacting with the user interface. According to another embodiment, a system is provided and includes a processor, a display device electronically coupled to the processor, and software loaded and/or stored in a storage device electronically coupled to the controller, a memory device electronically coupled to the controller, and/or the controller and programmed to display an interactive map of a droplet microactuator. According to yet another embodiment, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, a display device displaying a user interface electronically coupled to the controller, and software for executing a protocol loaded and/or stored in a storage device electronically coupled to the controller, a memory device electronically coupled to the controller, and/or the controller.

摘要翻译： 提供了用于控制液滴微致动器的系统。 根据一个实施例，提供了一种系统，其包括电子耦合到控制器的控制器，液滴微致动器以及显示电子耦合到控制器的用户界面的显示设备，其中该系统被编程和配置为允许用户实现 通过与用户界面交互来进行液滴操作。 根据另一个实施例，提供了一种系统，包括处理器，电子耦合到处理器的显示设备，以及加载和/或存储在电子耦合到控制器的存储设备中的软件，电子耦合到控制器的存储设备，以及 /或控制器，并被编程以显示液滴微致动器的交互式地图。 根据另一个实施例，提供了一种系统，其包括控制器，电子耦合到控制器的液滴微致动器，显示电子耦合到控制器的用户界面的显示设备，以及用于执行加载和/或存储在控制器中的协议的软件 电子耦合到控制器的存储设备，电子耦合到控制器的存储设备和/或控制器。

公开(公告)号：US20140174926A1

公开(公告)日：2014-06-26

申请号：US14115215

申请日：2012-05-01

申请人： Donovan Bort ,  Carrie Graham ,  Vamsee Pamula ,  Michael Pollack ,  Ramakrishna Sista ,  Vijay Srinivasan

发明人： Donovan Bort ,  Carrie Graham ,  Vamsee Pamula ,  Michael Pollack ,  Ramakrishna Sista ,  Vijay Srinivasan

IPC分类号： G01N27/447 ,  B81B1/00

CPC分类号： G01N27/44791 ,  B01F1/0027 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2001/0055 ,  B01F2215/0037 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  B81B1/004

摘要： A method of mixing a droplet, the method comprising providing a droplet on a surface, forming the droplet into a first “U” shape having a bottom region and two terminal ends, and simultaneously merging the terminal ends and splitting the droplet at the bottom region to form a second “U” shape which is substantially opposite the first “U” shape.

摘要翻译： 一种混合液滴的方法，所述方法包括在表面上提供液滴，将所述液滴形成为具有底部区域和两个末端的第一“U”形状，并同时合并末端并在底部区域分离液滴 以形成与第一“U”形大体相反的第二“U”形状。