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公开(公告)号：US20190195771A1
公开(公告)日：2019-06-27
申请号：US16230570
申请日：2018-12-21
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
CPC classification number: G01N15/1404 , G01N2015/0065 , G01N2458/15 , H01J49/004 , H01J49/04 , H01J49/0431
Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.
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公开(公告)号：US20190153511A1
公开(公告)日：2019-05-23
申请号：US16134855
申请日：2018-09-18
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Geoffrey Richard Facer , Barry Clerkson , Christopher G. Cesar , Neil Switz
CPC classification number: C12Q1/686 , B01J2219/00576 , B01J2219/00704 , B01L3/5027 , B01L7/52 , G01N21/64 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/75 , G01N21/8483 , G01N27/44721 , G01N2021/6421 , G01N2201/061 , G02B21/16 , G02B21/36
Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
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公开(公告)号：US10227624B2
公开(公告)日：2019-03-12
申请号：US14800548
申请日：2015-07-15
Applicant: Fluidigm Corporation
Inventor： Peilin Chen , Jing Wang , Andrew May
IPC: C12Q1/68 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/683
Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
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公开(公告)号：US10214773B2
公开(公告)日：2019-02-26
申请号：US15724190
申请日：2017-10-03
Applicant: Fluidigm Corporation
Inventor： Robert C. Jones
IPC: C12Q1/68 , C12Q1/686 , C12Q1/682 , G01N33/532 , G01N33/53
Abstract: Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids (e.g., RNA) and proteins.
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公开(公告)号：US10180386B2
公开(公告)日：2019-01-15
申请号：US15888871
申请日：2018-02-05
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
Abstract: A method for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. Particles or element tags associated with particles can be vaporized, atomized, and ionized, such as with an inductively coupled plasma device or a glow discharge device. The vaporized, atomized, and ionized particles or element tags can be analyzed using mass spectrometry, such as using a time of flight mass spectrometer or a magnetic sector mass spectrometer. The amount of at least one element in individual particles can be measured through mass analysis. The amount of many different tags, for example at least five different tags, can be measured at the same time to facilitate multi-parametric analysis of cells and other particles. The vaporized, atomized, and ionized particles or element tags can be pretreated in an ion pretreatment system to filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough.
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Patent Agency Ranking
公开(公告)号：US10131934B2
公开(公告)日：2018-11-20
申请号：US15805720
申请日：2017-11-07
Applicant: Fluidigm Corporation
Inventor： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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公开(公告)号：US20180195948A1
公开(公告)日：2018-07-12
申请号：US15888871
申请日：2018-02-05
Applicant: Fluidigm Corporation
Inventor： Dmitry R. Bandura , Vladimir I. Baranov , Scott D. Tanner
CPC classification number: G01N15/1404 , G01N2015/0065 , G01N2458/15 , H01J49/004 , H01J49/04 , H01J49/0431
Abstract: A method for cellular analysis of cellular particles tagged with elemental tags, such as lanthanide-based elemental tags. Particles or element tags associated with particles can be vaporized, atomized, and ionized, such as with an inductively coupled plasma device or a glow discharge device. The vaporized, atomized, and ionized particles or element tags can be analyzed using mass spectrometry, such as using a time of flight mass spectrometer or a magnetic sector mass spectrometer. The amount of at least one element in individual particles can be measured through mass analysis. The amount of many different tags, for example at least five different tags, can be measured at the same time to facilitate multi-parametric analysis of cells and other particles. The vaporized, atomized, and ionized particles or element tags can be pretreated in an ion pretreatment system to filter out low mass ions, such as using a high-pass mass filter or a bandpass mass filter, to allow the elemental tags to pass therethrough.
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公开(公告)号：US20180087092A1
公开(公告)日：2018-03-29
申请号：US15724190
申请日：2017-10-03
Applicant: Fluidigm Corporation
Inventor： Robert C. Jones
IPC: C12Q1/68 , G01N33/532
CPC classification number: C12Q1/686 , C12Q1/682 , G01N33/532 , G01N2458/10 , C12Q2521/101 , C12Q2531/113 , C12Q2537/125 , C12Q2545/114
Abstract: Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids (e.g., RNA) and proteins.
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公开(公告)号：US09926521B2
公开(公告)日：2018-03-27
申请号：US14188660
申请日：2014-02-24
Applicant: Fluidigm Corporation
Inventor： Antoine Daridon
IPC: C12M3/00 , B01L3/00 , C12M1/34 , C12M3/06 , G01N33/483 , G02B21/32 , G01N15/14 , G01N15/00 , G01N15/02
CPC classification number: C12M1/34 , B01L3/502738 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0636 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/06 , B01L2300/0636 , B01L2300/0645 , B01L2300/0681 , B01L2300/0861 , B01L2300/0867 , B01L2300/087 , B01L2300/088 , B01L2300/0887 , B01L2300/0893 , B01L2300/123 , B01L2400/0409 , B01L2400/0415 , B01L2400/0481 , B01L2400/0622 , B01L2400/0655 , C12M21/06 , C12M23/16 , G01N15/1484 , G01N33/4833 , G01N2015/008 , G01N2015/0288 , G01N2015/149 , G01N2015/1493 , G02B21/32
Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.
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公开(公告)号：US20180073051A1
公开(公告)日：2018-03-15
申请号：US15805720
申请日：2017-11-07
Applicant: Fluidigm Corporation
Inventor： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
CPC classification number: C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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