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177 条记录 (耗时 0.055 秒)

    Glycoprotein synthesis
    103.
    发明授权
    Glycoprotein synthesis 失效
    糖蛋白合成

    公开(公告)号：US06927042B2

    公开(公告)日：2005-08-09

    申请号：US10686944

    申请日：2003-10-15

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： A61K38/14 ,  A61K38/16 ,  C07K9/00 ,  C07K14/00 ,  C07K14/47 ,  C12N9/10 ,  C12P19/00 ,  C12P21/00 ,  C12P21/06

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    摘要翻译： 提供在体外和体内制备糖蛋白的方法。 一种方法包括将非天然氨基酸掺入蛋白质并将一个或多个糖部分连接到非天然氨基酸上。 另一种方法包括将包含糖部分的非天然氨基酸掺入蛋白质中。 通过两种方法制备的蛋白质可以用额外的糖进一步修饰。

    Scanning evanescent electro-magnetic microscope
    104.
    发明授权
    Scanning evanescent electro-magnetic microscope 失效
    扫描渐逝电磁显微镜

    公开(公告)号：US06532806B1

    公开(公告)日：2003-03-18

    申请号：US09695508

    申请日：2000-10-23

    申请人： Xiao-Dong Xiang ,  Chen Gao ,  Peter G. Schultz ,  Tao Wei

    发明人： Xiao-Dong Xiang ,  Chen Gao ,  Peter G. Schultz ,  Tao Wei

    IPC分类号： G01B734

    CPC分类号： G01Q60/22 ,  Y10S977/86 ,  Y10S977/864 ,  Y10S977/865 ,  Y10S977/869

    摘要： A novel scanning microscope is described that uses near-field evanescent electromagnetic waves to probe sample properties. The novel microscope is capable of high resolution imaging and quantitative measurements of the electrical properties of the sample. The inventive scanning evanescent wave electromagnetic microscope (SEMM) can map dielectric constant, tangent loss, conductivity, complex electrical impedance, and other electrical parameters of materials. The quantitative map corresponds to the imaged detail. The novel microscope can be used to measure electrical properties of both dielectric and electrically conducting materials.

    摘要翻译： 描述了一种新颖的扫描显微镜，其使用近场渐逝电磁波来探测样品特性。 该新型显微镜能够对样品的电性能进行高分辨率成像和定量测量。 本发明的扫描瞬逝波电磁显微镜（SEMM）可以绘制材料的介电常数，切线损耗，电导率，复电阻抗等电参数。 定量图对应于成像细节。 新型显微镜可用于测量电介质和导电材料的电学性能。

    Antibody-enhanced stereospecific hydrolyses
    106.
    发明授权
    Antibody-enhanced stereospecific hydrolyses 失效
    抗体增强的立体特异性水解

    公开(公告)号：US5190865A

    公开(公告)日：1993-03-02

    申请号：US574674

    申请日：1990-08-29

    申请人： Peter G. Schultz

    发明人： Peter G. Schultz

    IPC分类号： A61K47/48 ,  C07K16/00 ,  C12N9/00 ,  C12N9/86 ,  C12N15/10 ,  C12P21/08

    CPC分类号： C07K16/00 ,  A61K47/48369 ,  A61K47/48507 ,  C12N15/10 ,  C12N9/0002 ,  C12N9/86

    摘要： Hydrolyses of various chiral compounds are performed in stereospecific manner by antibodies generated from analogs of transition states of the compounds. The compounds susceptible to such hydrolysis include esters, thioesters and amides. In certain embodiments of the invention, antibodies with a strong selectivity toward one of the two enantiomers with respect to the chiral substrate are generated from haptens which is a mixture of enantiomers. A method of screening antibodies thus generated for those which are active towards the desired cleavage is also disclosed, using a test compound similar in structure to the intended substrate but which contains both a fluorescence donor group and a fluorescence quenching group, situated on opposite sides of the cleavage site. Cleavage by active antibodies results in separation of the groups and a resulting termination of the quenching effect.

    摘要翻译： 各种手性化合物的水解通过由化合物的过渡态的类似物产生的抗体以立体特异性方式进行。 对这种水解敏感的化合物包括酯，硫酯和酰胺。 在本发明的某些实施方案中，相对于手性底物，对于两种对映异构体之一具有强选择性的抗体是由对映异构体混合物的半抗原产生的。 还公开了筛选针对对所需切割有活性的抗体的抗体的方法，其使用与预期底物相似结构的测试化合物，但同时含有荧光供体基团和荧光猝灭基团，位于 切割位点。 通过活性抗体切割导致基团的分离和终止猝灭效应。

    COMPOSITIONS AND METHODS FOR INDUCING CELL DEDIFFERENTIATION
    108.
    发明申请
    COMPOSITIONS AND METHODS FOR INDUCING CELL DEDIFFERENTIATION 有权
    用于诱导细胞分化的组合物和方法

    公开(公告)号：US20130196989A1

    公开(公告)日：2013-08-01

    申请号：US13594524

    申请日：2012-08-24

    申请人： Shuibing Chen ,  Sheng Ding ,  Peter G. Schultz

    发明人： Shuibing Chen ,  Sheng Ding ,  Peter G. Schultz

    IPC分类号： C12N5/071 ,  C07D473/16

    CPC分类号： C12N5/0696 ,  C07D473/16 ,  C07D473/24

    摘要： The present invention provides compounds, compositions and methods for dedifferentiating lineage committed mammalian cells into stem cells. The present invention also provides methods of inducing dedifferentiation of lineage committed mammalian cells into stem cells, which can be further differentiated into various lineage committed cells. Methods of identifying additional compounds useful for inducing dedifferentiation of lineage committed cells into stem cells are also provided.

    摘要翻译： 本发明提供用于将分裂的哺乳动物细胞去分化为干细胞的化合物，组合物和方法。 本发明还提供了诱导分泌哺乳动物细胞去干细胞到干细胞中的方法，其可进一步分化成各种谱系确定的细胞。 还提供了鉴定另外的化合物的方法，该化合物可用于诱导谱系分化为干细胞的分化。

    Genetically programmed expression of selectively sulfated proteins in Eubacteria
    109.
    发明授权
    Genetically programmed expression of selectively sulfated proteins in Eubacteria 有权
    在细菌中选择性硫酸化蛋白质的遗传程序表达

    公开(公告)号：US08361770B2

    公开(公告)日：2013-01-29

    申请号：US13326143

    申请日：2011-12-14

    申请人： Chang C. Liu ,  Peter G. Schultz

    发明人： Chang C. Liu ,  Peter G. Schultz

    IPC分类号： C12P21/02 ,  C12N9/00 ,  C12N1/21

    CPC分类号： C12N9/93 ,  C07K14/815 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid sulfotyrosine and translation systems.

    摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸磺基酪氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交氨酰-tRNA合成酶，编码新合成酶分子的多核苷酸，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸磺酰酪氨酸的蛋白质的翻译方法和翻译系统。

    Systems for the expression of orthogonal translation components in eubacterial host cells
    110.
    发明授权
    Systems for the expression of orthogonal translation components in eubacterial host cells 有权
    在细胞宿主细胞中表达正交翻译组分的系统

    公开(公告)号：US08357512B2

    公开(公告)日：2013-01-22

    申请号：US13468736

    申请日：2012-05-10

    申请人： Youngha Ryu ,  Peter G. Schultz

    发明人： Youngha Ryu ,  Peter G. Schultz

    IPC分类号： C12N15/70 ,  C12N1/00 ,  C07H21/00

    CPC分类号： C07K14/245 ,  C12N15/70 ,  C12P21/02

    摘要： The invention related to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural acids at genetically-programmed positions.

    摘要翻译： 本发明涉及用于体内产生包含一种或多种非天然氨基酸的多肽的组合物和方法。 具体地，本发明提供了用于在遗传程序位置上有效地表达包含一种或多种非天然酸的多肽的多肽的质粒系统。