-
公开(公告)号：US10585090B2
公开(公告)日：2020-03-10
申请号：US16132175
申请日：2018-09-14
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Ramakrishna Sista , Arjun Sudarsan
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N35/00 , G01N1/00 , G01N1/10 , B01L3/00 , G01N33/543 , B01F13/00 , B03C1/02 , B03C1/28 , B03C1/30 , B03C5/00 , C12N15/10 , G01N33/561 , B01F11/00 , B01L7/00 , G01N35/10
Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
-
112.发明申请公开(公告)号：US20190060923A1
公开(公告)日：2019-02-28
申请号：US16176329
申请日：2018-10-31
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
CPC classification number: B05B5/0255 , B05B12/082 , G01F23/22 , G01F23/24 , G01F23/26
Abstract: The present invention is directed to methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
-
公开(公告)号：US09910010B2
公开(公告)日：2018-03-06
申请号：US14987352
申请日：2016-01-04
Applicant: Advanced Liquid Logic, Inc.
Inventor： William Craig Bauer
IPC: G01N27/447 , B01L7/00 , B01L3/00 , B01F13/00
CPC classification number: G01N27/44734 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/525 , B01L7/54 , B01L2200/027 , B01L2200/06 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0887 , B01L2300/12 , B01L2300/18 , B01L2300/1805 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , B01L2400/0677 , B01L2400/0683 , G01N27/44791
Abstract: The invention relates to a droplet actuator device and methods for integrated sample preparation and analysis of a biological sample. A droplet actuator device is provided for conducting droplet operations. The droplet actuator device may include a bottom substrate and a top substrate separated from each other to form a gap therebetween; an arrangement of droplet operations electrodes arranged on one or both of the bottom and/or top substrates for conducting droplet operations thereon; a reagent storage layer comprising one or more compartments bound to the top substrate; and one or more openings arranged to provide a fluidic path from the one or more compartments into the gap, upon breach of a breachable seal separating the one or more compartments and openings.
-
公开(公告)号：US09863913B2
公开(公告)日：2018-01-09
申请号：US14428219
申请日：2013-10-14
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Michael G. Pollack
IPC: G01N27/447 , B01L3/00 , B01L3/02
CPC classification number: G01N27/44791 , B01L3/0241 , B01L3/502792 , B01L2300/0816 , B01L2300/0867 , B01L2300/088 , B01L2300/0883 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , B01L2400/0457 , G01N27/44743
Abstract: A liquid handling system for supplying liquids to a flow cell (FC). The system may include a droplet actuator cartridge, wherein the droplet actuator and a flow cell are fluidly coupled to, or situated within, a droplet operations gap of the droplet actuator.
-
公开(公告)号：US09630180B2
公开(公告)日：2017-04-25
申请号：US14590470
申请日：2015-01-06
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Y. Paik
CPC classification number: B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration may include one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
-
Patent Agency Ranking
公开(公告)号：US20170080428A1
公开(公告)日：2017-03-23
申请号：US15367046
申请日：2016-12-01
Applicant: Advanced Liquid Logic, Inc. , Duke University
Inventor： Alexander D. Shenderov , Michael G. Pollack
IPC: B01L7/00
CPC classification number: B01L7/525 , B01L3/502792 , B01L2200/0673 , B01L2300/0654 , B01L2300/0864 , B01L2300/0887 , B01L2300/089 , B01L2300/1816 , B01L2300/1827 , B01L2300/1872 , B01L2400/0427
Abstract: Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.
-
公开(公告)号：US09545641B2
公开(公告)日：2017-01-17
申请号：US14870433
申请日：2015-09-30
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Theodore Winger
CPC classification number: B05B5/087 , B41J2/14 , B41J2/1606 , B41J2002/14322 , B41J2002/14395 , Y10T428/31504 , Y10T428/3154 , Y10T428/31855
Abstract: A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.
Abstract translation: 一种微流体装置，其具有使用在疏水层下面的导电油墨层制成的导电元件的基板。
-
公开(公告)号：US09545640B2
公开(公告)日：2017-01-17
申请号：US14580407
申请日：2014-12-23
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Theodore Winger
CPC classification number: B05B5/087 , B41J2/14 , B41J2/1606 , B41J2002/14322 , B41J2002/14395 , Y10T428/31504 , Y10T428/3154 , Y10T428/31855
Abstract: A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.
Abstract translation: 一种微流体装置，其具有使用在疏水层下面的导电油墨层制成的导电元件的基板。
-
公开(公告)号：US09476856B2
公开(公告)日：2016-10-25
申请号：US13890715
申请日：2013-05-09
Applicant: Advanced Liquid Logic Inc. , Duke University
Inventor： Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair
IPC: G01N33/543 , G01N27/72 , G01N27/74
CPC classification number: G01N27/72 , G01N27/745 , G01N33/54326
Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.
Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。
-
公开(公告)号：US20160216231A1
公开(公告)日：2016-07-28
申请号：US15087020
申请日：2016-03-31
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan
IPC: G01N27/447 , G01N33/543 , G01N15/10 , C12Q1/68
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
-
-
-
-
-
-
-
-
-
Search Results
129
records
(took 0.073 seconds)
