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公开(公告)号：US20160016172A1
公开(公告)日：2016-01-21
申请号：US14837133
申请日：2015-08-27
Applicant: ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY
Inventor： Michael G. Pollack , Philip Paik , Vamsee K. Pamula
IPC: B01L7/00 , G01N27/447
CPC classification number: C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.
Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。
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公开(公告)号：US09139865B2
公开(公告)日：2015-09-22
申请号：US13680979
申请日：2012-11-19
Applicant: Advanced Liquid Logic, Inc. , Duke University
Inventor： Michael G. Pollack , Philip Paik , Vamsee K. Pamula
IPC: G05D5/06 , C12Q1/68 , G01N27/447
CPC classification number: C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
Abstract: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.
Abstract translation: 本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由控制器执行指令的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。
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公开(公告)号：US20150072900A1
公开(公告)日：2015-03-12
申请号：US14498378
申请日：2014-09-26
Applicant: ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY
Inventor： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack
CPC classification number: B01L3/502784 , B01J19/0046 , B01J2219/00466 , B01J2219/00495 , B01J2219/00587 , B01J2219/00725 , B01L3/502761 , B01L2200/0642 , B01L2200/0647 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/0887 , B01L2300/0893 , B01L2300/18 , B01L2400/0427 , B01L2400/043 , C07K1/1136 , C30B7/00 , C30B29/58 , Y10T436/2575
Abstract: Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
Abstract translation: 提供样品处理液滴致动器，系统和方法。 根据一个实施例，提供了一种包括液滴微致动器的冲压装置，其包括：（a）包括用于在其表面上输送液滴的控制电极的路径或网络的第一板; （b）相对于第一板以基本上平行的方向安装的第二板，提供板之间的内部容积，第二板包括一个或多个冲压端口，用于将一些部分或全部液滴从内部容积输送到 外部位置; （c）用于将流体引入板之间的内部容积的端口; 和（d）对应于控制电极的路径或网络的参考电极的路径或网络。 还提供了包括冲压装置在内的相关系统和方法。
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公开(公告)号：US10585090B2
公开(公告)日：2020-03-10
申请号：US16132175
申请日：2018-09-14
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Ramakrishna Sista , Arjun Sudarsan
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N35/00 , G01N1/00 , G01N1/10 , B01L3/00 , G01N33/543 , B01F13/00 , B03C1/02 , B03C1/28 , B03C1/30 , B03C5/00 , C12N15/10 , G01N33/561 , B01F11/00 , B01L7/00 , G01N35/10
Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
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公开(公告)号：US09630180B2
公开(公告)日：2017-04-25
申请号：US14590470
申请日：2015-01-06
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Y. Paik
CPC classification number: B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration may include one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
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Patent Agency Ranking
公开(公告)号：US20160216231A1
公开(公告)日：2016-07-28
申请号：US15087020
申请日：2016-03-31
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan
IPC: G01N27/447 , G01N33/543 , G01N15/10 , C12Q1/68
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
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公开(公告)号：US09358551B2
公开(公告)日：2016-06-07
申请号：US14928096
申请日：2015-10-30
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Prasanna Thwar , Vamsee K. Pamula , Arjun Sudarsan , Ramakrishna Sista
CPC classification number: B03C7/026 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0887 , B01L2400/0424 , B01L2400/0427 , B03C1/01 , B03C1/288 , B03C5/00 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N33/54326
Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
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公开(公告)号：US20150330943A1
公开(公告)日：2015-11-19
申请号：US14805532
申请日：2015-07-22
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Michael G. Pollack , Vamsee K. Pamula , Vijay Srinivasan
IPC: G01N27/447
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.
Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了一种提供包括单个电池的液滴的方法，其中所述方法包括提供液滴，其包含由油介质包围的电润湿液滴致动器上的悬浮液，从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池，并且基于使用电润湿介导的液滴操作的确定步骤的结果，将分配的液滴分配在电润湿液滴致动器上。
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公开(公告)号：US09081007B2
公开(公告)日：2015-07-14
申请号：US14466193
申请日：2014-08-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N33/561 , B01F11/00 , B01F13/00 , B01L3/00 , B03C1/02 , B03C1/30 , B03C5/00 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
Abstract translation: 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。
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公开(公告)号：US20230128722A1
公开(公告)日：2023-04-27
申请号：US18063874
申请日：2022-12-09
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Ramakrishna Sista , Arjun Sudarsan
IPC: G01N33/543 , B01L3/00 , B03C1/02 , B03C1/28 , B03C1/30 , B03C5/00 , C12N15/10 , G01N33/561 , B01F31/65 , B01F33/302 , B01F33/3031
Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
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