公开(公告)号：US20060194307A1

公开(公告)日：2006-08-31

申请号：US11317004

申请日：2005-12-27

申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

IPC分类号： C12M1/34 ,  B07C5/00

CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

摘要： The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

摘要翻译： 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。

公开(公告)号：US20060141619A1

公开(公告)日：2006-06-29

申请号：US10544799

申请日：2004-02-19

申请人： Akihiro Hattori ,  Kenji Yasuda

发明人： Akihiro Hattori ,  Kenji Yasuda

IPC分类号： C12N5/06 ,  C12M1/00

CPC分类号： C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

摘要： There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, said absorption layer having absorbency in visible and infrared regions, and said gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and said light source having a monochromatic light in said specific wave length, wherein said light source is disposed such that it irradiates on said absorption layer and/or said gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on said transparent base plate.

摘要翻译： 提供了具有期望的培养空间的用于细胞培养的微室的处理装置和处理方法。 本发明的细胞培养用微细胞处理装置，具有不超过一个吸收层和至少一层凝胶层的微室，依次层叠在透明基板上，在可见光和红外线中不具有明显的吸收性 区域和至少一个光源，所述吸收层在可见光和红外区域具有吸收性，所述凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物 在具有特定波长的可见光和红外区域具有吸收能力，并且所述光源具有所述特定波长的单色光，其中所述光源被设置成使得其照射在所述吸收层和/或 所述凝胶层除了没有设置吸收层之外，将由凝胶状材料构成的至少两层层合在所述透明体b上 灰板

公开(公告)号：US20050170347A1

公开(公告)日：2005-08-04

申请号：US10499005

申请日：2001-12-19

申请人： Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

发明人： Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

IPC分类号： B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N27/414 ,  H01L29/06 ,  H01L29/66 ,  C12M1/34

CPC分类号： B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/5085 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  G01N27/4145 ,  C12Q2565/501 ,  C12Q2565/607

摘要： A DNA microarray system whereby measurement can be performed at a low running cost, a low price and yet a high accuracy. A nucleic acid probe (3) is immobilized on the surface of a gate insulator of an electric field effect transistor and then hybridized with a target gene on the surface of the gate insulator. A change in the surface electric charge density thus arising is detected by using the electric effect.

摘要翻译： 一种DNA微阵列系统，其可以以低运行成本，低价格和高精度进行测量。 将核酸探针（3）固定在电场效应晶体管的栅极绝缘体的表面上，然后与栅极绝缘体的表面上的目标基因杂交。 通过使用电效应来检测由此产生的表面电荷密度的变化。

公开(公告)号：US06716584B2

公开(公告)日：2004-04-06

申请号：US10086713

申请日：2002-03-04

申请人： Kenji Yasuda ,  Kazunori Okano ,  Hirokazu Kato

发明人： Kenji Yasuda ,  Kazunori Okano ,  Hirokazu Kato

IPC分类号： C12Q168

CPC分类号： C12Q1/6806 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5027 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/0877 ,  B01L2300/1822 ,  B01L2400/0421 ,  B01L2400/0487 ,  C12Q1/6837 ,  C40B40/06

摘要： Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.

公开(公告)号：US06168669A

公开(公告)日：2001-01-02

申请号：US09250394

申请日：1999-02-16

申请人： Kenji Yasuda ,  Naruaki Iida

发明人： Kenji Yasuda ,  Naruaki Iida

IPC分类号： C23C1600

CPC分类号： H01L21/68707

摘要： Supporting members are provided at three places, for example, on a frame section of a pair of tweezers. Each supporting member has a tapered face as an inclined guide for allowing a rim portion of a substrate to slide down to be guided to a predetermined position. A vertical wall which is formed continuing from the tapered face and being nearly perpendicular to a supported face of a wafer is provided at an upper end of the tapered face. Even when the rim portion of the wafer rises along the tapered face with the movement of the tweezers, the rim portion stops by hitting against the vertical wall, thereby preventing the wafer from falling from the tweezers.

摘要翻译： 支撑构件设置在三个位置，例如，在一对镊子的框架部分上。 每个支撑构件具有锥形面作为倾斜引导件，用于允许基板的边缘部分向下滑动以被引导到预定位置。 在锥面的上端设置有从锥形表面连续并且几乎垂直于晶片的支撑面的垂直壁。 即使当晶片的边缘部分随着镊子的移动而沿着锥形表面上升时，边缘部分通过撞击垂直壁而停止，从而防止晶片从镊子落下。

公开(公告)号：US6162356A

公开(公告)日：2000-12-19

申请号：US272353

申请日：1999-03-19

申请人： Yukiko Ikeda ,  Yoshishige Endo ,  Yasuhiro Yoshimura ,  Takao Terayama ,  Kenji Yasuda ,  Toshinari Sakurai ,  Tetsuo Yokoyama ,  Takanori Aono

发明人： Yukiko Ikeda ,  Yoshishige Endo ,  Yasuhiro Yoshimura ,  Takao Terayama ,  Kenji Yasuda ,  Toshinari Sakurai ,  Tetsuo Yokoyama ,  Takanori Aono

IPC分类号： B01D15/08 ,  C03C11/00 ,  C07H1/08

CPC分类号： B01J20/2803 ,  B01J20/103 ,  B01J20/28026 ,  B01J20/283 ,  B01J20/3234

摘要： Composite particles formed from resin particles and silica particles (amount of silica particles: 0.2 to 10 wt. % on the basis of the resin particles) in an apparatus for formation of composite particles are placed in a PTFE mold and heat treated to weld the resin particles to one another, thereby forming a structural element, which is dipped in an ethyl silicate solution containing monodispersed silica particles, then taken out of the solution and subjected to further heat treatment to form an ultimate structural element, which is sealed in a tip case, whereby a tip can be produced at a low cost and used to separate nucleic acid from a liquid sample for a short time at a high recovery rate without any defects such as breaking, etc.

摘要翻译： 在复合颗粒形成装置中由树脂颗粒和二氧化硅颗粒形成的复合颗粒（二氧化硅颗粒的数量（基于树脂颗粒的0.2至10重量％））放置在PTFE模具中并进行热处理以使树脂 从而形成结构元件，将其浸入含有单分散二氧化硅颗粒的硅酸乙酯溶液中，然后从溶液中取出并进行进一步的热处理以形成最终结构元件，其密封在尖端壳体 从而可以以低成本生产尖端，并且用于以高回收率将液体样品中的核酸与液体样品分离短时间而没有诸如破裂等缺陷。

公开(公告)号：US6140017A

公开(公告)日：2000-10-31

申请号：US37538

申请日：1998-03-10

申请人： Katsuo Koshimura ,  Tadaaki Tanaka ,  Noboru Shimada ,  Kenji Yasuda

发明人： Katsuo Koshimura ,  Tadaaki Tanaka ,  Noboru Shimada ,  Kenji Yasuda

IPC分类号： G03F7/033 ,  G03C1/73

CPC分类号： G03F7/033 ,  Y10S430/107 ,  Y10S430/108

摘要： A water-developable photosensitive resin composition which comprises:(1) a particulate copolymer comprising, as recurring units in the copolymer, (a) 10 to 95 mole % of an aliphatic conjugated diene unit, (b) 0.1 to 30 mole % of a monomer unit having at least one polymerizable unsaturated group and at least one functional group selected from the group consisting of carboxyl group, amino group, hydroxyl group, epoxy group, sulfonic acid group and phosphoric acid group, and (c) 0.1 to 20 mole % of a monomer unit having at least two polymerizable unsaturated groups;(2) at least one copolymer selected from the group consisting of (i) a block-like copolymer composed of two or more polymeric segments having a sulfonic acid group in at least one of the polymeric segments, (ii) a block-like copolymer composed of two or more polymeric segments having a polyurethane segment in at least one of the polymeric segments, and (iii) a copolymer of ethylene and an .alpha.-olefin having 3 to 20 carbon atoms;(3) a photopolymerizable unsaturated compound; and(4) a photopolymerization initiator.

摘要翻译： 一种水显影性感光性树脂组合物，其特征在于，含有：（1）作为共聚物中的重复单元的（a）10〜95摩尔％的脂肪族共轭二烯单元，（b）0.1〜30摩尔％ 具有至少一个可聚合不饱和基团和至少一个选自羧基，氨基，羟基，环氧基，磺酸基和磷酸基的官能团的单体单元，和（c）0.1-20摩尔％ 具有至少两个可聚合不饱和基团的单体单元; （2）至少一种共聚物，其选自（i）由两个或更多个在至少一个聚合物链段中具有磺酸基的聚合物链段组成的嵌段共聚物，（ii）嵌段共聚物 由两个或更多个在至少一个聚合物链段中具有聚氨酯链段的聚合物链段组成，和（iii）乙烯和具有3-20个碳原子的α-烯烃的共聚物; （3）光聚合性不饱和化合物; 和（4）光聚合引发剂。

公开(公告)号：US5597451A

公开(公告)日：1997-01-28

申请号：US490872

申请日：1995-06-15

申请人： Kenichi Nagai ,  Kenji Yasuda ,  Toshio Hama ,  Yoshitoshi Sekiguchi ,  Toshio Tachibana ,  Osamu Nakanishi ,  Tadashi Moriyama

发明人： Kenichi Nagai ,  Kenji Yasuda ,  Toshio Hama ,  Yoshitoshi Sekiguchi ,  Toshio Tachibana ,  Osamu Nakanishi ,  Tadashi Moriyama

IPC分类号： C10B53/07 ,  C10G1/10 ,  B01D45/00 ,  C10B27/00

CPC分类号： C10B53/07 ,  C10G1/10 ,  Y02P20/143

摘要： A thermal decomposition apparatus for plastics wherein plastics are melted and thermally decomposed, and the resulting decomposition gas is cooled for condensation and recovered in the form of a thermal decomposition oil. The apparatus comprises a thermal decomposition reactor for melting and thermally decomposing the plastics therein, an extraneous matter discharge duct having one end opened in a melt of plastics within the reactor, an extraneous matter collecting container connected to the other end of the discharge duct, and an aspirator for aspirating extraneous matter within the reactor together with the melt through the discharge duct into the container.

摘要翻译： 一种用于塑料的热分解装置，其中塑料被熔化和热分解，并将所得的分解气体冷却冷凝并以热分解油的形式回收。 该装置包括用于熔化和热分解其中的塑料的热分解反应器，一个外部物质排放管道，其一端在反应器内的塑料熔体中开口，一个连接到排放管道另一端的外部物质收集容器，以及 用于将反应器内的外来物质与熔体一起通过排放管吸入容器中的抽吸器。

公开(公告)号：US5213873A

公开(公告)日：1993-05-25

申请号：US600378

申请日：1990-10-19

申请人： Kenji Yasuda ,  Kazuaki Ohshima ,  Susumu Hasegawa

发明人： Kenji Yasuda ,  Kazuaki Ohshima ,  Susumu Hasegawa

IPC分类号： B41M5/52

CPC分类号： B41M5/5245 ,  Y10S428/913 ,  Y10S428/914 ,  Y10T428/259

摘要： An aqueous ink-jet recording sheet capable of recording clear, brilliant color images, and having a high water resistance, storage durability and resistance to curling and undulations, comprises a neutral paper substrate sheet having a Stoechigt sizing degree of 1 to 15 seconds; and an aqueous ink image-receiving layer in a basis weight of 0.5 to 10 g/m.sup.2 and comprising (a) fine, oil absorbing silica particles, (b) polyvinyl alcohol binder and (c) a water resisting agent comprising a cationic, water-soluble acrylic copolymer having side chains attached to a vinyl backbone chain and each having at least two cationic radicals.

公开(公告)号：US5143904A

公开(公告)日：1992-09-01

申请号：US552270

申请日：1990-07-13

申请人： Toshihiro Minato ,  Masaru Kato ,  Kenji Yasuda ,  Norio Yamamura ,  Masahiro Kamiya

发明人： Toshihiro Minato ,  Masaru Kato ,  Kenji Yasuda ,  Norio Yamamura ,  Masahiro Kamiya

IPC分类号： B41M5/00 ,  B41M5/41 ,  B41M5/52

CPC分类号： B41M5/41 ,  B41M2205/32 ,  B41M2205/38 ,  B41M5/44 ,  B41M5/52 ,  Y10S428/913 ,  Y10S428/914 ,  Y10T428/24942 ,  Y10T428/24967 ,  Y10T428/24975 ,  Y10T428/31 ,  Y10T428/31775 ,  Y10T428/31895 ,  Y10T428/31902 ,  Y10T428/31906

摘要： A dye image-receiving sheet for thermal transfer printing systems, comprising a substrtate sheet composed of a support paper sheet, a front coated layer comprising a thermoplastic resin, and optionally, a back coated layer comprising a thermoplastic resin; and a dye image-receiving layer comprising a resinous material capable of being dyed with a sublimating dye, and characterized in that the front coating layer has a Bekk smoothness of 100 seconds or more and the substrate sheet has a rigidity of 700 mgf or less.