公开(公告)号：US09958390B2

公开(公告)日：2018-05-01

申请号：US15362259

申请日：2016-11-28

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Yann Astier ,  Stephen W. Bedell ,  Ning Li ,  Devendra K. Sadana ,  William T. Spratt ,  Chao Wang

IPC: G01N21/64 ,  H01L31/0216 ,  H01L31/0232 ,  H01L31/0304 ,  H01L25/16 ,  H01L31/18 ,  H01L31/036 ,  G01N15/14 ,  G01N21/05 ,  B01L3/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N21/6428 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/12 ,  B01L2300/168 ,  G01N15/1436 ,  G01N15/1459 ,  G01N21/05 ,  G01N21/645 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2201/08 ,  H01L25/167 ,  H01L31/02162 ,  H01L31/02164 ,  H01L31/02322 ,  H01L31/03044 ,  H01L31/036 ,  H01L31/1856

Abstract: A semiconductor device used for fluorescent-based molecule detection and a method for manufacturing the same are provided. The semiconductor device has a fluid channel layer defining a fluid channel through which a sample stream flows. A target cell coupled with a fluorescent source is contained by the sample stream. The semiconductor device also has an excitation light source for generating excitation light that reaches the target cell coupled with the fluorescent source to generate fluorescent light. The semiconductor device also has a light filter layer for permitting the fluorescent light to pass through and to block the excitation light and a light detection layer for detecting the fluorescent light. The functional components of the device are highly integrated. Leakage of the excitation light and background noise into the light detection component can be minimized to improve the quality of detection.

公开(公告)号：US20160144405A1

公开(公告)日：2016-05-26

申请号：US14697072

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Benjamin H. Wunsch

IPC: B07B13/04 ,  G01N15/10

CPC classification number: B07B13/04 ,  B07B1/4609 ,  B82Y30/00 ,  G01N15/02 ,  G01N2015/0065 ,  G01N2015/0288 ,  Y10S977/84

Abstract: A technique relates sorting entities. The entities are introduced into a nanopillar array. The entities include a first population and a second population, and the nanopillar array includes nanopillars arranged to have a gap separating one from another. The nanopillars are ordered to have an array angle relative to a fluid flow direction. The entities are sorted through the nanopillar array by transporting the first population of the entities less than a predetermined size in a first direction and by transporting the second population of the entities at least the predetermined size in a second direction different from the first direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the entities having a sub-100 nanometer size.

公开(公告)号：US09346052B2

公开(公告)日：2016-05-24

申请号：US13971166

申请日：2013-08-20

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/487

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N27/44791 ,  Y10T29/49002

Abstract: A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.

公开(公告)号：US20150252414A1

公开(公告)日：2015-09-10

申请号：US14199248

申请日：2014-03-06

Applicant: International Business Machines Corporation

Inventor： Jingwei Bai ,  Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: C12Q1/68

CPC classification number: B01L3/502707 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0896 ,  B01L2300/12 ,  B01L2400/0415 ,  B01L2400/086 ,  B81B2201/058 ,  B81B2203/0338 ,  B81B2203/0392 ,  B81C1/00103 ,  B82Y40/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N33/48721

Abstract: A technique includes forming a gradient channel with width and depth gradients. A mask is disposed on top of a substrate. The mask is patterned with at least one elongated channel pattern having different elongated channel pattern widths. A channel is etched in the substrate in a single etching step, the channel having a width gradient and a corresponding depth gradient both simultaneously etched in the single etching step according to the different elongated channel pattern widths in the mask.

Abstract translation: 一种技术包括形成具有宽度和深度梯度的梯度通道。 掩模设置在基板的顶部。 掩模被图案化为具有不同细长通道图案宽度的至少一个细长通道图案。 在单个蚀刻步骤中在衬底中蚀刻沟道，根据掩模中不同的细长沟道图案宽度，通道具有在单个蚀刻步骤中同时蚀刻的宽度梯度和相应的深度梯度。

公开(公告)号：US20140151228A1

公开(公告)日：2014-06-05

申请号：US13969997

申请日：2013-08-19

Applicant: International Business Machines Corporation

Inventor： Ajay K. Royyuru ,  Chao Wang

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/414 ,  G01N27/447 ,  G01N33/48721 ,  G01N33/68 ,  Y10S977/733 ,  Y10S977/832 ,  Y10S977/853 ,  Y10S977/962

Abstract: A mechanism is provided for manipulating a molecule. The molecule is driven into a nanochannel filed with electrically conductive fluid. A first vertical electric field is created inside the nanochannel to slow down the molecule and/or immobilize the molecule. The molecule is stretched into non-folded linear chains by the first vertical electric field and a horizontal electric field. Monomers of the molecule are sequentially read.

Abstract translation: 提供了用于操纵分子的机制。 该分子被驱动到具有导电流体的纳米通道中。 在纳米通道内形成第一垂直电场，以减缓分子和/或固定分子。 通过第一垂直电场和水平电场将分子拉伸成非折叠线性链。 顺序读取分子的单体。

公开(公告)号：US20140151227A1

公开(公告)日：2014-06-05

申请号：US13690149

申请日：2012-11-30

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Ajay K. Royyuru ,  Chao Wang

IPC: G01N27/447

CPC classification number: G01N27/44791 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/414 ,  G01N27/447 ,  G01N33/48721 ,  G01N33/68 ,  Y10S977/733 ,  Y10S977/832 ,  Y10S977/853 ,  Y10S977/962

Abstract: A mechanism is provided for manipulating a molecule. The molecule is driven into a nanochannel filed with electrically conductive fluid. A first vertical electric field is created inside the nanochannel to slow down the molecule and/or immobilize the molecule. The molecule is stretched into non-folded linear chains by the first vertical electric field and a horizontal electric field. Monomers of the molecule are sequentially read.

公开(公告)号：US10464061B2

公开(公告)日：2019-11-05

申请号：US15096933

申请日：2016-04-12

Applicant: International Business Machines Corporation

Inventor： Jingwei Bai ,  Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: B81C1/00 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487 ,  C12Q1/6874 ,  B82Y40/00

Abstract: A technique includes forming a gradient channel with width and depth gradients. A mask is disposed on top of a substrate. The mask is patterned with at least one elongated channel pattern having different elongated channel pattern widths. A channel is etched in the substrate in a single etching step, the channel having a width gradient and a corresponding depth gradient both simultaneously etched in the single etching step according to the different elongated channel pattern widths in the mask.

公开(公告)号：US10330597B2

公开(公告)日：2019-06-25

申请号：US15498569

申请日：2017-04-27

Applicant: International Business Machines Corporation

Inventor： Yann Andre Nicolas Astier ,  Ning Li ,  Devendra K. Sadana ,  Chao Wang

IPC: G01N21/05 ,  G01N21/64

Abstract: Apparatus for enhancing on-chip fluorescence detection. For example, an apparatus comprises a microfluidic channel, an excitation signal enhancing structure formed on a first side of the microfluidic channel and a photodetector structure formed on a second side of the microfluidic channel. For example, the excitation signal enhancing structure enhances an excitation signal and the enhanced excitation signal excites one or more samples in the microfluidic channel to emit signals at a fluorescence wavelength at a higher rate.

公开(公告)号：US09983190B2

公开(公告)日：2018-05-29

申请号：US15085394

申请日：2016-03-30

Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION

Inventor： Qinghuang Lin ,  Gustavo A. Stolovitzky ,  Chao Wang ,  Deqiang Wang

IPC: G01N33/487 ,  B01L3/00

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/12 ,  B01L2300/042 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N27/44791 ,  Y10T29/49002

Abstract: A device for passing a biopolymer molecule includes a nanochannel formed between a surface relief structure, a patterned layer forming sidewalls of the nanochannel and a sealing layer formed over the patterned layer to encapsulate the nanochannel. The surface relief structure includes a three-dimensionally rounded surface that reduces a channel dimension of the nanochannel at a portion of nanochannel and gradually increases the dimension along the nanochannel toward an opening position, which is configured to receive a biopolymer.

公开(公告)号：US09636675B2

公开(公告)日：2017-05-02

申请号：US14697095

申请日：2015-04-27

Applicant: International Business Machines Corporation

Inventor： Yann A. Astier ,  Robert L. Bruce ,  Joshua T. Smith ,  Chao Wang ,  Benjamin H. Wunsch

IPC: B81C1/00 ,  B01L3/00 ,  B03B5/48 ,  B81B1/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/10 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2400/086 ,  B03B5/48 ,  B81B1/006 ,  B81B2201/058 ,  B81B2203/0361 ,  B81C1/00111 ,  B81C2201/0132 ,  B82B3/0014 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G03F7/0002 ,  G03F7/2037

Abstract: A technique related to sorting entities is provided. An inlet is configured to receive a fluid, and an outlet is configured to exit the fluid. A nanopillar array, connected to the inlet and the outlet, is configured to allow the fluid to flow from the inlet to the outlet. The nanopillar array includes nanopillars arranged to separate entities by size. The nanopillars are arranged to have a gap separating one nanopillar from another nanopillar. The gap is constructed to be in a nanoscale range.