公开(公告)号：US20250109428A1

公开(公告)日：2025-04-03

申请号：US18899460

申请日：2024-09-27

Applicant: Illumina, Inc.

Inventor： Olivia Ghazinejad ,  Fei Shen ,  Kyle Bentz ,  Rohit Subramanian ,  Eric Brustad ,  Lena Storms ,  Xiaoyu Ma ,  Mathieu Lessard-Viger ,  Jeffrey Brodin

IPC: C12Q1/6806 ,  B01L3/00 ,  C12Q1/6844

Abstract: In some examples, a hybrid particle for use in capturing a polynucleotide may include a scaffold molecule including a seeding primer and a plurality of first moieties, and a nanoparticle including a plurality of second moieties. The scaffold molecule is coupled to the nanoparticle via interactions between the plurality of first moieties and the plurality of second moieties.

公开(公告)号：US20240425907A1

公开(公告)日：2024-12-26

申请号：US18749217

申请日：2024-06-20

Applicant: ILLUMINA, INC.

Inventor： Niall Gormley ,  Anurag Agrawal ,  Mathieu Lessard-Viger ,  Jonathan Boutell ,  Eli Carrami

IPC: C12Q1/6841 ,  C12Q1/6874

Abstract: The present disclosure is generally directed to strategies for spatial multiomics via target nucleic acid capture and amplification.

公开(公告)号：US20240132956A1

公开(公告)日：2024-04-25

申请号：US18476151

申请日：2023-09-27

Applicant: ILLUMINA, INC.

Inventor： Justin Robbins ,  Mathieu Lessard-Viger

IPC: C12Q1/6874 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0896 ,  B01L2300/12

Abstract: An example of a nucleic acid sequencing component includes a support. A glycolipid bi-layer is attached to at least a portion of the support. First and second primers are respectively attached to the glycolipid bi-layer. In one example, the support is a substrate of a flow cell. In another example, the support is a core nanostructure that can be introduced into a flow cell.

公开(公告)号：US20240011080A1

公开(公告)日：2024-01-11

申请号：US18000663

申请日：2021-12-20

Applicant: Illumina, Inc.

Inventor： Hayden Black ,  Esteban Armijo ,  Mathieu Lessard-Viger

IPC: C12Q1/6837

CPC classification number: C12Q1/6837

Abstract: A composition for capturing target polynucleotides at a surface of a substrate is provided. The composition may include a plurality of capture primers coupled to the surface of the substrate and including modified nucleic acids; and a plurality of orthogonal capture primers coupled to the surface of the substrate and including modified nucleic acids. The modified nucleic acids of the capture primers may include locked nucleic acid (LNA), peptide nucleic acid (PNA), or super T. The modified nucleic acids of the orthogonal capture primers may include locked nucleic acid (LNA), peptide nucleic acid (PNA), or super T.

公开(公告)号：US11787930B2

公开(公告)日：2023-10-17

申请号：US16721451

申请日：2019-12-19

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C12Q1/6869 ,  C12Q1/6853 ,  C08L33/26

CPC classification number: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US20200224020A1

公开(公告)日：2020-07-16

申请号：US16721451

申请日：2019-12-19

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US20250084477A1

公开(公告)日：2025-03-13

申请号：US18575479

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Esteban Armijo ,  Jonathan Mark Boutell ,  Jeffrey Brodin ,  Eli M. Carrami ,  Maria Candelaria Rogert Bacigalupo ,  Randall Smith ,  Mathieu Lessard-Viger ,  Ludovic Vincent

IPC: C12Q1/6876

Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3′ end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3′ end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.

公开(公告)号：US12172156B2

公开(公告)日：2024-12-24

申请号：US18233289

申请日：2023-08-11

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01L3/00 ,  B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  C40B50/18 ,  C08L37/00 ,  G01N21/05

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

公开(公告)号：US12152237B2

公开(公告)日：2024-11-26

申请号：US17525553

申请日：2021-11-12

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Gianluca Andrea Artioli ,  Mathieu Lessard-Viger ,  Brian D. Mather ,  Xavier von Hatten

IPC: C12N15/10 ,  C08F265/10 ,  C08J3/075

Abstract: An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.

公开(公告)号：US20230381733A1

公开(公告)日：2023-11-30

申请号：US18233289

申请日：2023-08-11

Applicant: ILLUMINA, INC.

Inventor： Lewis J. Kraft ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Arnaud Rival ,  Justin Fullerton ,  M. Shane Bowen ,  Hui Han ,  Jeffrey S. Fisher ,  Yasaman Farshchi ,  Mathieu Lessard-Viger

IPC: B01J19/00 ,  C12N15/10 ,  C40B40/08 ,  B01L3/00 ,  C40B50/18

CPC classification number: B01J19/0046 ,  C12N15/1065 ,  C40B40/08 ,  C08L37/00 ,  C40B50/18 ,  B01L3/502707 ,  B01L3/5027

Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.