公开(公告)号：US10533209B2

公开(公告)日：2020-01-14

申请号：US15884902

申请日：2018-01-31

Applicant: Waters Technologies Corporation

Inventor： Joomi Ahn ,  Moon Chul Jung ,  Kevin D. Wyndham

IPC: C12Q1/37 ,  C12N11/06 ,  C12N11/14 ,  C12M1/40 ,  C12M1/34 ,  C12Q1/48

Abstract: An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.

公开(公告)号：US09488551B2

公开(公告)日：2016-11-08

申请号：US14375337

申请日：2013-02-01

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Pamela C. Iraneta ,  Kevin D. Wyndham ,  Moon Chul Jung

IPC: G01N1/00 ,  G01N1/40 ,  G01N33/49 ,  G01N1/10 ,  B01L3/00 ,  G01N1/28 ,  G01N35/00

CPC classification number: G01N1/00 ,  B01L3/5025 ,  B01L3/5635 ,  B01L2300/069 ,  B01L2300/0851 ,  B01L2300/087 ,  B01L2300/12 ,  B01L2400/0677 ,  G01N1/10 ,  G01N1/28 ,  G01N1/4055 ,  G01N33/49 ,  G01N2001/288 ,  G01N2001/4061 ,  G01N2035/00108

Abstract: Described are sample carriers and methods of extracting a fluid sample from a dried sample on a sample carrier. The sample carrier has a first carrier portion that receives a fluid sample, and includes a material that adsorbs the fluid sample and dissolves upon application of a solvent. A second carrier portion is attached to the first carrier portion and includes a material that is indissolvable in the solvent. The first carrier portion having a dried sample is dissolved into a solution upon application of the solvent. The solution can be analyzed to determine constituents in the extracted sample. The sample carrier can conveniently be in the form of a card or a dipstick. In alternative embodiments, a carrier portion that includes the dried sample is indissolvable in the solvent but is released from another carrier portion through application of a solvent to an intervening dissolvable carrier portion.

Abstract translation: 描述了样品载体和从样品载体上的干燥样品中提取流体样品的方法。 样品载体具有接收流体样品的第一载体部分，并且包括吸附流体样品并在施加溶剂时溶解的材料。 第二载体部分附接到第一载体部分并且包括在溶剂中不溶解的材料。 具有干燥样品的第一载体部分在施加溶剂时溶解于溶液中。 可以分析溶液以确定提取的样品中的成分。 样品载体可以方便地以卡片或量油尺的形式。 在替代实施例中，包括干燥样品的载体部分在溶剂中是可溶解的，但是通过将溶剂施加到中间可溶解载体部分而从另一个载体部分释放。

公开(公告)号：US09482603B2

公开(公告)日：2016-11-01

申请号：US14345009

申请日：2012-09-18

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Pamela C. Iraneta ,  Erin E. Chambers ,  Moon Chul Jung

IPC: B01L3/00 ,  G01N1/40 ,  B01L7/00 ,  B01L9/06 ,  B01L9/00 ,  G01N1/28 ,  B01L3/02

CPC classification number: G01N1/405 ,  B01L3/0217 ,  B01L3/5021 ,  B01L3/5023 ,  B01L3/5085 ,  B01L3/523 ,  B01L7/52 ,  B01L9/06 ,  B01L9/52 ,  B01L2200/028 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/082 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0677 ,  G01N1/4055 ,  G01N2001/288 ,  G01N2001/4061

Abstract: Described is a device for solid phase extraction of a dried sample of a biological fluid. The device includes a support structure such as a plate or support block that has a first side to receive a dried sample card. The support structure also has a second side that is opposite the first side and a number of apertures that extend between the first and second sides. The device also includes a plurality of wells each containing a sample preparation material and having an inlet end that communicates with a respective aperture at the second side of the support structure. Each dried sample on the dried sample card is aligned with one of the apertures. Extraction samples in each well pass through the sample preparation material, such as a chromatographic sorbent, in the well to remove one or more constituents of the extraction sample.

Abstract translation: 描述了用于固相萃取生物流体干燥样品的装置。 该装置包括支撑结构，例如板或支撑块，其具有接收干燥样品卡的第一侧。 支撑结构还具有与第一侧相对的第二侧和在第一和第二侧之间延伸的多个孔。 该装置还包括多个孔，每个孔各自包含样品制备材料，并且具有与支撑结构的第二侧处的相应孔连通的入口端。 干燥的样品卡上的每个干燥的样品与其中一个孔对准。 每个孔中的提取样品通过孔中的样品制备材料，例如色谱吸附剂，以除去萃取样品中的一种或多种组分。

公开(公告)号：US11905548B2

公开(公告)日：2024-02-20

申请号：US17881175

申请日：2022-08-04

Applicant: Waters Technologies Corporation

Inventor： Joomi Ahn ,  Moon Chul Jung ,  Kevin D. Wyndham

IPC: C12Q1/37 ,  C12N11/06 ,  C12N11/14 ,  C12M1/40 ,  C12M1/34 ,  C12Q1/48

CPC classification number: C12Q1/37 ,  C12M21/18 ,  C12M41/40 ,  C12N11/06 ,  C12N11/14 ,  C12Q1/48

Abstract: An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in a fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.

公开(公告)号：US20190277816A1

公开(公告)日：2019-09-12

申请号：US16292610

申请日：2019-03-05

Applicant: Waters Technologies Corporation

Inventor： Abhijit Tarafder ,  Moon Chul Jung ,  Edouard S.P. Bouvier

IPC: G01N30/60 ,  B01L3/00

Abstract: A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel.

公开(公告)号：US20180161770A1

公开(公告)日：2018-06-14

申请号：US15892863

申请日：2018-02-09

Applicant: Waters Technologies Corporation

Inventor： Pamela J. Longenbach ,  Moon Chul Jung ,  Frank J. Marszalkowski, JR. ,  Geoff Gerhardt

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L3/5453 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2400/0478 ,  G01N1/38 ,  G01N2001/288

Abstract: Provided are devices and methods for sample collection and processing. A tubular body has a chamber closed at a first end by a thin penetrable barrier and open at a second end opposite the first end. The tubular body further includes a partition that divides the chamber into two adjacent compartments, wherein a first compartment of the two adjacent compartments has the partition facing opposite the open end of the chamber and a second compartment of the two adjacent compartments has the partition facing opposite the closed end of the chamber. The partition has one or more holes to allow passage of fluid from the first compartment into the second compartment. A tracking identification may be permanently affixed to the first end of the tubular body.

公开(公告)号：US09927331B2

公开(公告)日：2018-03-27

申请号：US14388274

申请日：2013-03-04

Applicant: Waters Technologies Corporation

Inventor： Xin Zhang ,  Pamela C. Iraneta ,  Moon Chul Jung ,  Pamela J. Longenbach ,  Frank Marszalkowski, Jr.

IPC: B01L3/00 ,  G01N1/30 ,  G01N33/543 ,  G01N33/96 ,  G01N1/10

CPC classification number: G01N1/30 ,  B01L3/5088 ,  B01L2300/126 ,  B01L2300/161 ,  G01N1/10 ,  G01N33/5436 ,  G01N33/96 ,  G01N2291/02466

Abstract: Described is a biological sample carrier based on a rigid hydrophilic object fabricated from a non-absorbing inert porous material. A biological fluid sample applied to the surface of the object is absorbed into the pores and constituents of the biological fluid sample remain in the pores after drying. Unlike a disc punched from a conventional dried blood spot (DBS) card, the object is easily handled. In contrast to DBS cards containing glass fiber structures that interact with basic analytes and other DBS card having adsorbing fibers that tend to separate blood constituents as the sample spreads through the fibers, the biological sample carrier is substantially inert to drug analytes.

公开(公告)号：US20150266019A1

公开(公告)日：2015-09-24

申请号：US14434316

申请日：2013-09-25

Applicant: Waters Technologies Corporation

Inventor： Pamela J. Longenbach ,  Moon Chul Jung ,  Frank John Marszalkowski, JR.

IPC: B01L3/00

CPC classification number: B01L3/5082 ,  B01L3/502 ,  B01L2200/0631 ,  B01L2300/047 ,  B01L2300/0609 ,  B01L2300/0618 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/087 ,  B01L2300/105 ,  B01L2400/0457 ,  B01L2400/0478 ,  G01N1/4055 ,  G01N30/06 ,  G01N2001/4061 ,  G01N2030/062

Abstract: Described is a matrix spot processing device comprising a tubular apparatus. An absorbent media is located either separate from, or attached to, the tubular apparatus. A sample is spotted on the absorbent media. A reservoir is in the tubular apparatus. A first region of the reservoir is constructed and arranged to have an extraction solvent. A second region of the reservoir, which may or may not be the same as the first region, is constructed and arranged to receive an extract generated from an interaction between the extraction solvent and the spotted sample at the absorbent media.

Abstract translation: 描述了一种包括管状装置的矩阵点处理装置。 吸收介质与管状设备分开或连接到管状设备上。 样品被点在吸收介质上。 管状设备中有一个储存器。 储存器的第一区域被构造和布置成具有提取溶剂。 储存器的第二区域可以或可以不与第一区域相同，被构造和布置成接收由吸收介质上的提取溶剂和点样样品之间的相互作用产生的提取物。

公开(公告)号：US20220203360A1

公开(公告)日：2022-06-30

申请号：US17335373

申请日：2021-06-01

Applicant: Waters Technologies Corporation

Inventor： Moon Chul Jung

IPC: B01L3/00

Abstract: A vial cap for a sample vial includes a cylindrical cap body defining a first circular opening at a first end. The vial cap also includes a cap lid extending radially inward from a second end of the cylindrical cap body. The cap lid defines a second circular opening. The vial cap also includes a septum located within the cylindrical cap body and in contact with the cap lid. The septum spans the second circular opening defined by the cap lid portion. The vial cap also includes a number of flexible threads extending from an internal surface of the cylindrical cap body.

公开(公告)号：US20220082532A1

公开(公告)日：2022-03-17

申请号：US17471449

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Susan Rzewuski ,  Thomas H. Walter ,  Matthew A. Lauber ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/16 ,  G01N30/86 ,  G01N30/72 ,  G01N30/74

Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.