公开(公告)号：US20170267970A1

公开(公告)日：2017-09-21

申请号：US15444413

申请日：2017-02-28

申请人： Whitehead Institute for Biomedical Research

发明人： Piyush GUPTA ,  Daniel MILLER ,  Ethan SOKOL

IPC分类号： C12N5/00 ,  A61L27/26 ,  A61L27/54 ,  A01K67/027 ,  C12N5/071 ,  G01N33/50 ,  C12N5/09 ,  C12N5/0793 ,  A61L27/52 ,  A61L27/38

CPC分类号： A01K67/0271 ,  A01K2207/12 ,  A01K2267/03 ,  A61L27/26 ,  A61L27/28 ,  A61L27/3633 ,  A61L27/3813 ,  A61L27/383 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/414 ,  A61L2300/416 ,  C08L5/08 ,  C12N5/0068 ,  C12N5/0619 ,  C12N5/0631 ,  C12N5/0693 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/999 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80 ,  G01N33/5011 ,  G01N33/5082 ,  C08L89/06

摘要： The presently disclosed subject matter provides hydrogel precursor compositions (e.g., solutions) for forming three-dimensional hydrogels that support growth of physiologically relevant tissue when at least one cell is cultured in the three-dimensional hydrogel, kits comprising the hydrogel precursor composition, three-dimensional hydrogels, methods of forming the three-dimensional hydrogels, methods of growing the physiologically relevant tissue using the three-dimensional hydrogels, physiologically relevant tissue grown in the three-dimensional hydrogels, methods of producing hormone-responsive tissue (e.g., milk-producing mammary tissue and related methods of producing milk), methods of screening for candidate agents useful for modulating hormonal responses (e.g., modulating milk production), method of screening for candidate therapeutic agents using the physiologically relevant tissue grown in the three-dimensional hydrogels (e.g., personalized cancer treatments), and related methods of treatment (e.g., administering agents identified using the methods herein, transplanting physiologically relevant tissue produced using the methods, etc.).

公开(公告)号：US09732329B2

公开(公告)日：2017-08-15

申请号：US15053992

申请日：2016-02-25

申请人： University of Technology, Sydney

发明人： Ann Margaret Simpson ,  Chang Tao

IPC分类号： C12N5/071 ,  A61K35/407 ,  A61K38/45 ,  C12N9/12 ,  A61K38/28 ,  C07K14/62 ,  C12N15/85 ,  A61K35/12 ,  A61K48/00

CPC分类号： C12N9/1205 ,  A61K35/12 ,  A61K35/407 ,  A61K38/28 ,  A61K38/45 ,  A61K48/00 ,  C07K14/62 ,  C12N5/067 ,  C12N15/85 ,  C12N2501/33 ,  C12N2510/00 ,  C12N2830/20 ,  C12Y207/01002

摘要： The present invention relates generally to a population of cells genetically modified to produce insulin in a glucose responsive manner and uses thereof. More particularly, the present invention relates to a population of cells genetically modified to produce insulin in response to physiologically relevant levels of glucose and uses thereof. The cells of the present invention are useful in a wide variety of applications, in particular in the context of therapeutic and prophylactic regimes directed to the treatment of diabetes and/or the amelioration of symptoms associated with diabetes, based on the transplantation of the cells of the present invention into mammals requiring treatment. Also facilitated is the design of in vitro based screening systems for testing the therapeutic effectiveness and/or toxicity of potential adjunctive treatment regimes.

公开(公告)号：US09708584B2

公开(公告)日：2017-07-18

申请号：US14360621

申请日：2012-12-03

申请人： R. BIO CO., LTD.

发明人： Sung Keun Kang ,  Jeong Chan Ra ,  Hyeong Geun Park ,  Hang Young Lee

IPC分类号： C12N5/077 ,  C12N5/0775

CPC分类号： C12N5/0667 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/39

摘要： The present invention relates to a medium composition for transforming stem cells from an aged person into young stem cells, and more particularly to a medium composition for culturing stem cells, which is used to rejuvenate stem cells from an aged person so as to have characteristics similar to those of the stem cells of young people, and to a method for rejuvenating stem cells, which comprises culturing stem cells from an aged person in the medium composition. According to the present invention, even mesenchymal stem cells collected from over 60 years old patients can be transformed into young mesenchymal stem cells having high differentiation ability, high telomerase activity, and high ability to express stem cell markers. Thus, the present invention can significantly increase the efficacy of cell therapy employing mesenchymal stem cells.

公开(公告)号：US09694107B2

公开(公告)日：2017-07-04

申请号：US15166059

申请日：2016-05-26

申请人： TWO CELLS, CO., LTD.

发明人： Norimasa Nakamura ,  Hideki Yoshikawa ,  Wataru Ando

IPC分类号： C12N5/00 ,  A61L27/36 ,  A61L27/38 ,  C07K14/47 ,  C12N5/077 ,  A61L27/24 ,  A61L27/50 ,  A61K35/12

CPC分类号： A61L27/3612 ,  A61K35/12 ,  A61L27/24 ,  A61L27/3633 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61L27/50 ,  A61L2430/06 ,  C07K14/47 ,  C12N5/0654 ,  C12N5/0655 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/20 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/999 ,  C12N2502/1311 ,  C12N2506/1384

摘要： The present invention can be used for actual implantation surgery without a scaffold. The present invention provides a synthetic tissue or complex which can be produced by culture and has a high level of differentiation ability. The present invention also provides a therapy and medicament for repairing and/or regenerating tissue using replacement and covering. By culturing cells under specific culture conditions such that medium contains an extracellular matrix synthesis promoting agent, the cells are organized and are easily detached from a culture dish. The present invention was achieved by finding such a phenomenon. In addition, the self contraction of the tissue can be regulated by culturing the tissue in a suspended manner. Therefore, it is possible to regulate the three-dimensional shape of the tissue. The present invention also provides a method for producing an implantable synthetic tissue which does not require a plurality of monolayer cell sheets assembled to form a three-dimensionally structured synthetic tissue. The present invention is characterized by richness in adhesion molecules, nonnecessity of additional fixation at an implantation site, and good biological integration.

公开(公告)号：US09683989B2

公开(公告)日：2017-06-20

申请号：US14512557

申请日：2014-10-13

申请人： Technion Research & Development Foundation Limited

发明人： Shy Shoham ,  Anat Marom ,  Sanjeev Kumar Mahto

IPC分类号： C12M1/34 ,  G01N33/50 ,  A61N5/06 ,  A61K35/30 ,  C12M1/00 ,  C12N5/0793 ,  A61B17/11 ,  A61L27/38 ,  A61L27/52

CPC分类号： G01N33/5058 ,  A61B17/1128 ,  A61F2230/0063 ,  A61K35/30 ,  A61L27/383 ,  A61L27/52 ,  A61N5/0622 ,  C12M31/00 ,  C12N5/0619 ,  C12N5/062 ,  C12N2501/13 ,  C12N2501/33 ,  C12N2503/04 ,  C12N2510/00 ,  C12N2513/00 ,  C12N2533/90

摘要： A neural network is disclosed. The neural network comprises a plurality of optogenetically modified neural cells being three-dimensionally distributed in a hydrogel medium and being disconnected from any solid support having a shear modulus above 1 GPa.

公开(公告)号：US20170152472A1

公开(公告)日：2017-06-01

申请号：US15205908

申请日：2016-07-08

申请人： University of North Carolina at Chapel Hill

发明人： Hiroshi KUBOTA ,  Lola M. REID

IPC分类号： C12N5/00 ,  C12N5/071

CPC分类号： C12N5/0018 ,  C12N5/067 ,  C12N5/0672 ,  C12N2500/00 ,  C12N2500/05 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/00 ,  C12N2501/11 ,  C12N2501/235 ,  C12N2501/33 ,  G01N33/56966

摘要： The present invention relates to precursor cells to hepatic stellate cells, compositions comprising same and methods of isolating same. The surface antigenic profile of the precursors is MHC class Ia negative, ICAM-1+, VCAM-1+, β3-integrin+. In addition to expression of these surface markers, the cells also express the intracellular markers desmin, vimentin, smooth muscle α-actin, nestin, hepatocyte growth factor, stromal derived factor-1α and Hlx homeobox transcriptional factor.

公开(公告)号：US20170081638A1

公开(公告)日：2017-03-23

申请号：US15120432

申请日：2015-02-27

申请人： FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

发明人： TENG MA

IPC分类号： C12N5/0775 ,  A61K35/28 ,  C12M3/04 ,  C12N5/00 ,  C12M3/06

CPC分类号： C12N5/0663 ,  A61K35/28 ,  C12M25/16 ,  C12M27/10 ,  C12M27/16 ,  C12M33/00 ,  C12N5/0062 ,  C12N5/0075 ,  C12N2500/02 ,  C12N2500/42 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2509/00 ,  C12N2513/00 ,  C12N2527/00 ,  C12N2531/00 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2539/10

摘要： The subject invention concerns novel and translatable materials and methods for expansion of stem cells, such as mesenchymal stem cells (MSC), that significantly improve translational success of the cells in the treatment of various conditions, such as stroke. The subject invention utilizes cell self-aggregation as a non-genetic means to enhance their therapeutic potency in a microcarrier bioreactor. The subject invention integrates a cell aggregation process in a scalable bioreactor system. In one embodiment of the method, thermally responsive microcarriers (TRMs) are utilized in conjunction with a bioreactor system. Cells are cultured in a container or vessel in the presence of the TRMs wherein cells adhere to the surface of the TRMs. Once cells are adhered to the TRMs they can be cultured at a suitable temperature for cell growth and expansion, e.g., at about 37° C. After a period of time sufficient for cell growth and expansion on the TRMs, the cell culture temperature is reduced so that the cells detach from the TRMs. The detached cells are allowed to form cell clusters that are then cultured under conditions such that the clusters aggregate to form 3D aggregates. The 3D aggregates can be collected and treated to dissociate the cells (e.g., using enzymatic treatment, such as trypsinization). Dissociated cells can then be used for transplantation in methods of treatment or for in vitro characterization and study.

公开(公告)号：US20170081634A1

公开(公告)日：2017-03-23

申请号：US15368359

申请日：2016-12-02

申请人： Janssen Biotech, Inc.

发明人： Alireza Rezania

IPC分类号： C12N5/071

CPC分类号： C12N5/0608 ,  C12N5/0676 ,  C12N5/0678 ,  C12N2500/90 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02

摘要： The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 85% of the cells in the population express markers characteristic of the definitive endoderm lineage.

公开(公告)号：US20160355787A1

公开(公告)日：2016-12-08

申请号：US15173316

申请日：2016-06-03

申请人： ViaCyte, Inc.

发明人： Kevin A. D'Amour ,  Anne Bang ,  Emmanuel E. Baetge

IPC分类号： C12N5/071 ,  A61K35/39

CPC分类号： C12N5/0676 ,  A61K35/39 ,  C12N5/0678 ,  C12N2500/38 ,  C12N2501/10 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/58 ,  C12N2501/734 ,  C12N2501/999 ,  C12N2506/02

摘要： Disclosed herein are cell cultures and enriched cell populations of endocrine precursor cells, immature pancreatic hormone-expressing cells and mature pancreatic hormone-expressing cells. Also disclosed herein are methods of producing such cell cultures and cell populations.

摘要翻译： 本文公开了内分泌前体细胞，未成熟的表达胰腺激素的细胞和成熟的胰腺激素表达细胞的细胞培养物和富集的细胞群。 本文还公开了产生这种细胞培养物和细胞群体的方法。

公开(公告)号：US20160289643A1

公开(公告)日：2016-10-06

申请号：US15040770

申请日：2016-02-10

申请人： Georgetown University

发明人： Richard Schlegel ,  Xuefeng Liu

IPC分类号： C12N5/071

CPC分类号： C12N5/0683 ,  C12N5/0625 ,  C12N5/0631 ,  C12N5/067 ,  C12N5/0688 ,  C12N2500/05 ,  C12N2500/40 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2503/02 ,  C12N2510/04 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/5091

摘要： The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.