公开(公告)号：US10202571B2

公开(公告)日：2019-02-12

申请号：US14746494

申请日：2015-06-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James Q. Boedicker ,  Rustem F. Ismagilov ,  Hyun Jung Kim

IPC: C12M1/42 ,  C12M1/14 ,  C12N1/20 ,  C12N1/38 ,  C12N11/04 ,  C12P39/00 ,  C12Q1/18 ,  G01N33/542 ,  G01N33/543 ,  C12M1/00 ,  C12M1/12

Abstract: This invention provides devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.

公开(公告)号：US10196684B2

公开(公告)日：2019-02-05

申请号：US15030202

申请日：2014-10-16

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Bing Sun ,  Jesus Rodriguez Manzano ,  Eugenia Khorosheva ,  Matthew S. Curtis

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q1/70

Abstract: The present invention relates to assays, including amplification assays, conducted in the presence of modulators. These assays can be used to detect the presence of particular nucleic acid sequences. In particular, these assays can allow for genotyping or other genetic analysis.

公开(公告)号：US20160362734A1

公开(公告)日：2016-12-15

申请号：US15247795

申请日：2016-08-25

Applicant: California Institute of Technology ,  University of Chicago

Inventor： Rustem F. Ismagilov ,  Feng Shen ,  Jason E. Kreutz ,  Bing Sun ,  Wenbin Du

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: C12Q1/6851 ,  B01F13/0094 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2300/0864 ,  B01L2300/0896 ,  C12Q2531/119 ,  C12Q2537/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Provided are devices and methods for effecting processing of samples, including essentially isothermal amplification of nucleic acids, at multiple reaction locations in a single device. In some embodiments, the disclosed devices and methods provide for effecting parallel sample processing in several hundred locations on a single device.

Abstract translation: 提供了用于在单个装置中的多个反应位置处理样品的装置和方法，包括基本上等温的核酸扩增。 在一些实施例中，所公开的设备和方法提供了在单个设备上在几百个位置中实现并行样本处理。

公开(公告)号：US20150247190A1

公开(公告)日：2015-09-03

申请号：US14433602

申请日：2013-10-04

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  David A. Selck ,  Mikhail Karymov ,  Bing Sun

IPC: C12Q1/68 ,  G01N21/64 ,  C12Q1/70

CPC classification number: C12Q1/6851 ,  C12Q1/703 ,  C12Q2600/16 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/061 ,  G01N2201/062 ,  G01N2201/12 ,  G16B15/00 ,  C12Q2527/101 ,  C12Q2565/619 ,  C12Q2565/628

Abstract: Disclosed herein are methods and devices for assessing sample for the presence of a disease or organism using images from devices such as a consumer cell phones.

Abstract translation: 本文公开了使用来自诸如消费者手机的装置的图像评估疾病或生物体存在的样品的方法和装置。

公开(公告)号：US11760698B2

公开(公告)日：2023-09-19

申请号：US17560956

申请日：2021-12-23

Applicant: California Institute of Technology

Inventor： Katherine T. Faber ,  Julia A. Kornfield ,  Noriaki Arai ,  Orland Bateman ,  Rustem F. Ismagilov

IPC: C04B38/06 ,  B01D67/00 ,  B01D69/02 ,  B01D71/02 ,  B01J35/04 ,  B01J35/10 ,  C04B38/00 ,  H01B1/14 ,  H01B1/16

CPC classification number: C04B38/0605 ,  B01D67/0051 ,  B01D69/02 ,  B01D71/024 ,  B01J35/04 ,  B01J35/1071 ,  B01J35/1076 ,  C04B38/007 ,  C04B38/0038 ,  C04B38/0054 ,  C04B38/0093 ,  H01B1/14 ,  H01B1/16 ,  B01D2325/021 ,  B01D2325/26

Abstract: Provided herein are methods for making a freeze-cast material having a internal structure, the methods comprising steps of: determining the internal structure of the material, the internal structure having a plurality of pores, wherein: each of the plurality of pores has directionality; and the step of determining comprises: selecting a temperature gradient and a freezing front velocity to obtain the determined internal structure based on the selected temperature gradient and the selected freezing front velocity; directionally freezing a liquid formulation to form a frozen solid, the step of directionally freezing comprising: controlling the temperature gradient and the freezing front velocity to match the selected temperature gradient and the selected freezing front velocity during directionally freezing; wherein the liquid formulation comprises at least one solvent and at least one dispersed species; and subliming the at least one solvent out of the frozen solid to form the material.

公开(公告)号：US11624062B2

公开(公告)日：2023-04-11

申请号：US16141901

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Matthew S. Curtis ,  Mary Arrastia ,  David A. Selck ,  Mitchell Guttman

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806 ,  C40B30/04 ,  C40B40/08 ,  C40B70/00

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20180105859A1

公开(公告)日：2018-04-19

申请号：US15715086

申请日：2017-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Nathan G. Schoepp ,  Travis S. Schlappi ,  Matthew S. Curtis

IPC: C12Q1/18 ,  G01N33/58 ,  C12Q1/6851 ,  G01N33/569

CPC classification number: C12Q1/18 ,  C12Q1/10 ,  C12Q1/6851 ,  G01N33/56938 ,  G01N33/582 ,  C12Q2527/101 ,  C12Q2537/165

Abstract: Disclosed herein are methods and devices for using digital isothermal amplification to detect subtle responses to environmental stimuli, such as detecting antibiotic susceptibility using digital quantification of DNA replication and/or chromosome segregation.

公开(公告)号：US09822356B2

公开(公告)日：2017-11-21

申请号：US13868028

申请日：2013-04-22

Applicant: SLIPCHIP CORPORATION ,  CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Feng Shen ,  Liang Li ,  Yu-Hsiang Hsu ,  Stefano Begolo ,  Mikhail Karymov ,  David Selck ,  Stephanie McCalla ,  Philip James Homewood

IPC: G01N21/75 ,  C12N15/10 ,  B01L3/00 ,  G01N33/52 ,  B01L7/00

CPC classification number: C12N15/1003 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502784 ,  B01L3/52 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/0678 ,  B01L2300/045 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/065 ,  G01N33/525

Abstract: The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preparing and/or analyzing samples (e.g., biospecimen samples) by using one or more of capture regions and/or automated analysis.

公开(公告)号：US09803237B2

公开(公告)日：2017-10-31

申请号：US13869856

申请日：2013-04-24

Applicant: California Institute of Technology ,  SlipChip Corporation

Inventor： Rustem F. Ismagilov ,  Feng Shen

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/065

Abstract: The present invention relates to fluidic devices for compartmentalizing samples. In particular, the devices and related systems and methods allow for compartmentalization by using one or more first chambers connect by a first channel (e.g., where the cross-sectional dimension of the first channel is less than the cross-sectional dimension of at least one first chamber).

公开(公告)号：US20170299483A1

公开(公告)日：2017-10-19

申请号：US15485431

申请日：2017-04-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Erik Jue ,  Nathan Schoepp

IPC: G01N1/38 ,  B33Y10/00 ,  C12Q1/68 ,  B33Y80/00 ,  B01L3/00

CPC classification number: G01N1/38 ,  B01F5/0619 ,  B01L3/502 ,  B01L2200/026 ,  B01L2200/0621 ,  B01L2200/12 ,  B01L2400/0478 ,  B01L2400/0633 ,  B29C64/00 ,  B33Y10/00 ,  B33Y80/00 ,  C12Q1/6806 ,  G01N2001/382

Abstract: Devices and methods for biological sample preparation are provided herein. Components of such devices include a plurality of plungers that can be actuated for metering and/or mixing one or more agents. Methods of manufacturing such devices using 3D printing are also provided.