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公开(公告)号：US06358387B1
公开(公告)日：2002-03-19
申请号：US09536274
申请日：2000-03-27
申请人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
IPC分类号： C02F140
CPC分类号： G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
摘要翻译： 分析系统和方法使用模块化接口结构来提供样品基底和分析单元之间的界面，其中分析单元通常具有用于实现各种分析和控制功能的特定界面布置。 使用模块化接口结构的每个模块的许多变体有利地提供了使用具有特定分析和控制系统接口布置的特定基板或类别的基板执行大量测试的成本有效和有效的方式。 还提供了用于同时分析多个并行微通道中的反应或条件的改进的光学照明和检测系统。 通过使用包括多个激发频率的激发束同时照射非法线入射的多个平行微通道并且同时在垂直于衬底的方向上检测来自微通道中的物质的发射，本发明提供了增加的吞吐量和改进的排放物检测 使用具有多个检测器的检测模块。
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22.发明授权Apparatus and methods for correcting for variable velocity in microfluidic systems 有权用于校正微流体系统中可变速度的装置和方法公开(公告)号：US06703205B2
公开(公告)日：2004-03-09
申请号：US10102149
申请日：2002-03-19
申请人： Anne R. Kopf-Sill , Andrea W. Chow , Claudia B. Cohen , Steven A. Sundberg , John Wallace Parce
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Claudia B. Cohen , Steven A. Sundberg , John Wallace Parce
IPC分类号： G01N33558
CPC分类号： G01N33/557 , B01J19/0093 , B01J2219/0095 , B01J2219/0097 , B01J2219/00995 , B01L3/5027 , B01L3/50273 , B01L3/502746 , B01L3/563 , B01L9/527 , B01L2200/14 , B01L2200/143 , B01L2300/0627 , B01L2300/0816 , B01L2300/0867 , B01L2400/0415 , G01N27/447 , G01N27/44726 , G01N27/44791 , Y10S435/81 , Y10S436/805 , Y10S436/809
摘要： Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
摘要翻译： 提供了具有用于校正电动效果的计算机的电动装置。 还提供了通过在电动微流体装置中确定反应物和产物在反应中的速度来校正电动效应的方法。 这些微流体装置可以具有用于移动，混合和监测精确量的分析物流体的通道，凹陷和/或孔的基底。
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公开(公告)号：US06511853B1
公开(公告)日：2003-01-28
申请号：US09630866
申请日：2000-08-02
申请人： Anne R. Kopf-Sill , Andrea W. Chow
发明人： Anne R. Kopf-Sill , Andrea W. Chow
IPC分类号： G01N33558
CPC分类号： B01F13/0071 , B01F13/0093 , C12Q1/6813 , G01N27/44743 , Y10S366/02 , C12Q2565/629
摘要： Throughput rates for microfluidic serial analysis systems are optimized by maximizing the proximity and speed with which multiple different samples may be serially introduced into a microfluidic channel network. Devices are included that include optimized parameters based upon desired throughput rates for a given set of reagents, reaction times and the like.
摘要翻译： 微流控串行分析系统的吞吐率通过最大化接近度和速度来优化，通过该接近度和速度可以将多个不同的样品串行引入微流体通道网络。 包括装置，其包括针对给定的一组试剂，反应时间等的期望的通过速率的优化参数。
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公开(公告)号：US06506609B1
公开(公告)日：2003-01-14
申请号：US09569747
申请日：2000-05-11
申请人： H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow
发明人： H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow
IPC分类号： G01N700
CPC分类号： B01F13/0062 , B01J19/0093 , B01J2219/00889 , B01J2219/00891 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2400/0406 , B01L2400/0487 , G01N15/1404 , G01N27/44704 , G01N27/44791 , G01N2015/1409 , G01N2015/149 , G01N2333/4718 , Y10T436/117497 , Y10T436/2575
摘要： Methods and systems for particle focusing to increase assay throughput in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.
摘要翻译： 提供了用于增加微尺度系统中测定通过量的粒子聚焦的方法和系统。 本发明包括为微流体装置中的流动颗粒提供基本均匀的流速的方法。 还提供了分选颗粒群体成员的方法，例如细胞和各种亚细胞组分。 另外包括其中聚焦和/或分选颗粒的集成系统。
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公开(公告)号：US07192559B2
公开(公告)日：2007-03-20
申请号：US09922224
申请日：2001-08-02
CPC分类号： B01D61/18 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L2200/027 , B01L2300/0861 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0457 , Y10T137/2496 , Y10T137/2499 , Y10T137/2501 , Y10T137/2514 , Y10T137/2516 , Y10T436/11 , Y10T436/2575
摘要： Methods and devices for delivering fluids into microfluidic device body structures are described. The methods and devices include the use of fluid manifolds which are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.
摘要翻译： 描述了将流体输送到微流体装置体结构中的方法和装置。 所述方法和装置包括使用与装置主体结构集成或可互换的流体歧管。 还提供了制造歧管的方法。
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26.发明授权Microfluidic devices, systems and methods for performing integrated reactions and separations 失效用于执行集成反应和分离的微流体装置，系统和方法公开(公告)号：US06551836B1
公开(公告)日：2003-04-22
申请号：US09442073
申请日：1999-11-15
IPC分类号： G01N27447
CPC分类号： B01L3/50273 , B01F13/0059 , B01L3/502753 , B01L3/502784 , B01L2200/0605 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , G01N27/44743 , G01N27/44791 , G01N2001/4038 , Y10T436/117497 , Y10T436/25375 , Y10T436/2575
摘要： Microfluidic devices for performing integrated reaction and separation operations. The devices have a planar substrate having a first surface with an integrated channel network disposed therein. The reaction region in the integrated microscale channel network has a mixture of at least first and second reactants located therein, wherein the mixture interacts to produce one or more products. The reaction region is configured to maintain contact between the first and second reactants contained within it. The device also includes a separation region in the integrated channel network, where the separation region is configured to separate the first reactant from the product, when the first reactant and product are flowing through the separation region.
摘要翻译： 用于进行集成反应和分离操作的微流体装置。 这些装置具有平面基板，其具有设置在其中的集成通道网络的第一表面。 集成微量通道网络中的反应区域具有位于其中的至少第一和第二反应物的混合物，其中混合物相互作用以产生一种或多种产物。 反应区域被配置为保持包含在其中的第一和第二反应物之间的接触。 该装置还包括在集成通道网络中的分离区域，其中当第一反应物和产物流过分离区域时，分离区域被配置为将第一反应物与产物分离。
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公开(公告)号：US06500323B1
公开(公告)日：2002-12-31
申请号：US09277367
申请日：1999-03-26
IPC分类号： G01N27447
CPC分类号： G01N27/44791 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L2300/0816 , B01L2300/0838 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084
摘要： Methods and systems for designing optimized fluidic channel networks for performing different analytical operations, which include the steps of selecting a driving force, identifying at least a first reaction parameter, and designing the channel network by determining channel lengths and cross-sectional dimensions that are optimized for the reaction requirements in view of the selected driving force. Preferred methods are used to design integrated microscale fluidic systems.
摘要翻译： 用于设计用于执行不同分析操作的优化流体通道网络的方法和系统，其包括选择驱动力，识别至少第一反应参数以及通过确定经优化的通道长度和横截面尺寸来设计通道网络的步骤 鉴于所选驱动力的反应要求。 优选的方法用于设计集成微量流体系统。
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公开(公告)号：US6150119A
公开(公告)日：2000-11-21
申请号：US233700
申请日：1999-01-19
申请人： Anne R. Kopf-Sill , Andrea W. Chow
发明人： Anne R. Kopf-Sill , Andrea W. Chow
IPC分类号： G01N31/20 , C12Q1/25 , C12Q1/68 , G01N27/447 , G01N33/53 , G01N33/566 , G01N35/08
CPC分类号： B01F13/0071 , B01F13/0093 , C12Q1/6813 , G01N27/44743 , Y10S366/02
摘要： Throughput rates for microfluidic serial analysis systems are optimized by maximizing the proximity and speed with which multiple different samples may be serially introduced into a microfluidic channel network. Devices are included that include optimized parameters based upon desired throughput rates for a given set of reagents, reaction times and the like.
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公开(公告)号：US06858185B1
公开(公告)日：2005-02-22
申请号：US09645104
申请日：2000-08-23
CPC分类号： B01L3/5027 , B01F13/0059 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2400/0415 , B01L2400/049 , B01L2400/084 , G01N1/38 , G01N27/44791 , Y10T436/117497 , Y10T436/118339 , Y10T436/25625 , Y10T436/2575
摘要： Flow rates in a microfluidic device are modulated after performing serial dilutions by flow reduction channels that draw fluid from the main channel, thus reducing the flow rate. The reduction in flow rate and/or use of smaller dimension channels allow reduced reagent consumption. In addition, multiple flow reduction channels are used for multiple concentration measurements and for performing multiple assays simultaneously on a single sample. Also included are microfluidic devices and integrated systems for performing assays using serial dilutions, single pressure sources, multiple concentration measurements, and reduced reagent consumption. Devices comprising flow reduction channels are also used to suppress pressure perturbations from spontaneous injection.
摘要翻译： 在通过从主通道抽取流体的流动减少通道进行连续稀释之后调节微流体装置中的流速，从而降低流速。 较小尺寸通道的流速和/或使用的减少允许减少试剂消耗。 此外，多个流量减少通道用于多个浓度测量，并且在单个样品上同时进行多个测定。 还包括微流体装置和用于使用连续稀释，单一压力源，多重浓度测量和减少的试剂消耗进行测定的集成系统。 包括流动减少通道的装置也用于抑制自发注射的压力扰动。
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公开(公告)号：US06613580B1
公开(公告)日：2003-09-02
申请号：US09609030
申请日：2000-06-30
IPC分类号： C01N110
CPC分类号： G01N27/44791 , B01J19/0093 , B01J2219/00317 , B01J2219/00702 , B01J2219/00889 , B01J2219/00891 , B01J2219/00952 , B01J2219/00995 , B01L3/5027 , C12Q1/00 , C12Q1/686 , C40B60/14 , G01N27/44726 , G01N2500/00 , Y10T436/25 , Y10T436/25625 , Y10T436/2575 , C12Q2565/629
摘要： Methods, systems, kits, and apparatus for calculating kinetic and concentration information in microscale systems are provided. Dwell times for access by a microfluidic system are varied and the resulting modulation of signal profile information used to provide a first order estimation of an activity of a potential activity modulator accessed by the system.
摘要翻译： 提供了用于计算微量系统中的动力学和浓度信息的方法，系统，试剂盒和装置。 由微流体系统访问的停留时间是变化的，并且所得到的信号分布信息的调制用于提供系统访问的潜在活动调制器的活动的一阶估计。
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