公开(公告)号：US20240238789A1

公开(公告)日：2024-07-18

申请号：US18545690

申请日：2023-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Zergiebel

IPC: B01L3/00 ,  B01L3/02 ,  B01L9/00

CPC classification number: B01L3/527 ,  B01L3/0293 ,  B01L3/50855 ,  B01L3/523 ,  B01L9/543 ,  B01L2300/0825 ,  B01L2300/0858

Abstract: A reagent container includes a base defining a plurality of wells having openings exposed at an upper surface of the base and a tube receptacle. The plurality of wells include a first set of wells and a second set of wells. Each well of the first set of wells has an opening to a well bore and a channel in communication with the well bore. The opening has a first portion disposed over the well bore and a second portion disposed over the channel. The first portion has a larger area than the second portion. An angle defined by tangents to the inner surface of the first and second portions at a junction between the first and second portions is at least 100° and not greater than 180°. The reagent container further includes a top coupled over the top surface of the base and defining windows providing access to the openings of the plurality of wells and the tube receptacle.

公开(公告)号：US20240228785A1

公开(公告)日：2024-07-11

申请号：US18419897

申请日：2024-01-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Aimei CHEN ,  Daniel BEACHAM ,  Chrisgen VONNEGUT ,  Krishnamurthy NACHARAJU

IPC: C09B11/24 ,  G01N21/64

CPC classification number: C09B11/24 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure provides for compounds of Formula (I),




its corresponding compounds of Formula (II)




or salts thereof and their use as fluorogenic pH sensors.

公开(公告)号：US12031916B2

公开(公告)日：2024-07-09

申请号：US17962123

申请日：2022-10-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mingsong Chen ,  Mauro Aguanno ,  Kuan Moon Boo ,  Woon Liang Terence Soh ,  Jacob K. Freudenthal ,  Jeffrey A. Marks

IPC: G01N21/64 ,  G01N21/03 ,  G01N21/27

CPC classification number: G01N21/6486 ,  G01N21/0332 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2201/061 ,  G01N2201/0624 ,  G01N2201/0627 ,  G01N2201/121

Abstract: An instrument for processing and/or measuring a biological process comprises a sample processing system and an excitation source exhibiting a spectral function of output power or intensity verses wavelength of output power or intensity. The spectral function has a minima wavelength corresponding to a local minima value of the output power or intensity; a first maxima wavelength corresponding to a first local maxima of output power or intensity, the output power or intensity at the first local maxima being greater than the output power or intensity at any wavelength less than the minima wavelength; a second maxima wavelength corresponding to a second local maxima of output power or intensity, the output power or intensity at the second local maxima being greater than the output at any wavelength greater than the minima wavelength; the minima wavelength is between the first maxima wavelength and the second maxima wavelength.

公开(公告)号：US20240219388A1

公开(公告)日：2024-07-04

申请号：US18391779

申请日：2023-12-21

Applicant: Pierce Biotechnology, Inc. ,  Life Technologies Corporation ,  Bender MedSystems GmbH

Inventor： Paul Haney ,  Surbhi Desai ,  Marie Murakami ,  Penny Jensen ,  Brian Steer ,  Kelli Feather-Henigan ,  Syrus Jaffe ,  Brian Webb ,  Korin Pathammavong ,  Ricarda Heintz ,  Jordan Thompson ,  Jeremy Weaver ,  Carina Wimer ,  Andrew Westergren ,  Kai Chun Chen

IPC: G01N33/573 ,  C12N15/10 ,  G01N1/34 ,  G01N33/68

CPC classification number: G01N33/573 ,  C12N15/101 ,  G01N1/34 ,  G01N33/68 ,  G01N2333/90203 ,  G01N2333/98

Abstract: Methods for fractionating a sample of biomolecules of the disclosure comprise (a) introducing a sample into a separating column; (b) separating biomolecules in the sample by molecular weight into a plurality of fractions along the separating column; and (c) placing the separating column into successive engagement with a plurality of wells and advancing into each of the wells one or more of the corresponding plurality of fractions. Methods of the disclosure further comprise detecting and identifying biomolecules in the fractions using microparticles to bind to the fractions, and novel approaches to pool the fractions followed by assays—such as immunoassays—that allow high throughput and automation capabilities.

公开(公告)号：US20240201126A1

公开(公告)日：2024-06-20

申请号：US18536131

申请日：2023-12-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James BUSTILLO ,  Mark J. MILGREW ,  Wolfgang HINZ ,  John LEAMON ,  John DAVIDSON ,  Martin HUBER ,  Antoine M. VAN OIJEN ,  Jonathan ROTHBERG

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6874 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11998910B2

公开(公告)日：2024-06-04

申请号：US17412376

申请日：2021-08-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  C12Q1/6874 ,  B01L2200/026 ,  B01L2200/06 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0848

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

公开(公告)号：US11996169B2

公开(公告)日：2024-05-28

申请号：US17808880

申请日：2022-06-24

Applicant: Life Technologies Corporation

Inventor： Nivedita Sumi Majumdar

IPC: G16B45/00 ,  G06F16/16 ,  G06F16/26 ,  G06T11/20 ,  G16B20/00 ,  G16B40/00

CPC classification number: G16B45/00 ,  G06F16/168 ,  G06F16/26 ,  G06T11/206 ,  G16B20/00 ,  G16B40/00

Abstract: In one exemplary embodiment, a method computer-implemented method of generating an outlier wheel data visualization for a graphical user interface (GUI) is provided. The method includes receiving emission data from a plurality of amplification reactions. The method further includes generating an outlier wheel data visualization using the emission data including a plurality of lines, where each line included in the outlier wheel data visualization represents emission data from an amplification reaction of the plurality of amplification reactions, and each line having a length and a visual indicator. The length of each line represents growth of intensity of fluorescence of the emission data and the visual indicator indicates the associated cycle number of the emission data. Further, each line is associated with an angular position so that the plurality of lines is configured in a circular shape. The method includes displaying the outlier wheel data visualization on a GUI.

公开(公告)号：US20240159702A1

公开(公告)日：2024-05-16

申请号：US18353017

申请日：2023-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  Kim L. JOHNSON ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N33/543 ,  H01L27/088 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/78 ,  H01L29/42324 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US11982620B2

公开(公告)日：2024-05-14

申请号：US17441258

申请日：2020-03-17

Applicant: Life Technologies Corporation

Inventor： Shaohong Wang

IPC: G01N21/64 ,  G01N21/17 ,  G01N21/33

CPC classification number: G01N21/645 ,  G01N21/33 ,  G01N2021/174 ,  G01N2021/6463

Abstract: An optical detection system for a capillary electrophoresis instrument is disclosed. The system includes an ultraviolet (UV) source and an absorption measurement optical path. In an embodiment, the optical path comprises a first plurality of optical elements arranged to obtain a plurality of respective UV beamlets from a UV beam emitted by the UV source and to direct the respective UV beamlets transversely through respective capillaries of a plurality of capillaries and to an absorption detector positioned to detect respective signals for use in obtaining respective UV absorption measurements corresponding to the respective capillaries.

公开(公告)号：US11959074B2

公开(公告)日：2024-04-16

申请号：US17525737

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Kylan Szeto ,  Shanti Shankar ,  Mark Beauchemin

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.