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公开(公告)号：US06495369B1
公开(公告)日：2002-12-17
申请号：US09545241
申请日：2000-04-07
Applicant: Joseph E. Kercso , Steven A. Sundberg , Jeffrey A. Wolk , Andrew W. Toth , Calvin Y. H. Chow , J. Wallace Parce
Inventor： Joseph E. Kercso , Steven A. Sundberg , Jeffrey A. Wolk , Andrew W. Toth , Calvin Y. H. Chow , J. Wallace Parce
IPC: G01N3502
CPC classification number: G01N35/028 , G01N2035/00148 , Y10T436/11 , Y10T436/113332 , Y10T436/119163 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multi-well microtiter plates or other array structures. The multi-well plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multi-well plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and/or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and/or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.
Abstract translation: 本发明提供用于分析标准多孔微量滴定板或其它阵列结构中所含的大量样品化合物的改进的系统，装置和方法。 多孔板沿着输送系统行进到具有微流体装置的测试台。 在测试台处，每个板从输送机中取出，多孔板的孔依次与微流体装置的输入端对准。 在每个样品的至少一部分已经被输入到微流体通道系统中之后，板返回到输送系统。 可以沿输送机系统布置前后测试站，并且使用XYZ机器人臂和新的板支撑支架允许孔中的每个样品通过固定在微流体上的探针输入微流体网络 芯片。 具有铰接盖的蛤壳结构可以可释放地支撑芯片，同时提供和/或容纳微流体装置和周围系统之间的电，光学，结构和其它界面连接。
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32.发明授权Microfabricated structures for facilitating fluid introduction into microfluidic devices 有权用于促进流体引入微流体装置的微型结构公开(公告)号：US06451188B1
公开(公告)日：2002-09-17
申请号：US09539671
申请日：2000-03-30
Applicant: Steven A. Sundberg , J. Wallace Parce , Calvin Y. H. Chow
Inventor： Steven A. Sundberg , J. Wallace Parce , Calvin Y. H. Chow
IPC: G01N2726
CPC classification number: G01N27/44791 , B01J19/0093 , B01J2219/00783 , B01J2219/00826 , B01J2219/00831 , B01J2219/00833 , B01J2219/00853 , B01J2219/00891 , B01J2219/00909 , B01L3/0244 , B01L3/0262 , B01L3/5025 , B01L3/50255 , B01L3/5027 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502753 , B01L2200/027 , B01L2300/0681 , B01L2300/0816 , B01L2300/0819 , B01L2300/0864 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0688 , G01N27/44743 , G01N2035/00237 , G01N2035/1037 , Y10T436/25125 , Y10T436/25375 , Y10T436/2575
Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.
Abstract translation: 通过使用完全延伸通过微流体衬底的端口来促进流体引入。 可以使用毛细管力将流体保持在端口内，并且可以通过单个端口引入一系列样品或其它流体，通过依次将流体通过基底吹出，并用替代流体代替去除的流体，或者通过置换 流体部分附加流体。 在另一方面，微流体衬底具有在横截面尺寸上变化的通道，使得毛细管作用仅在通道网络的有限部分内扩散流体。 在另一方面，引入端口可以包括从端口引导到流体通道的多个非常小的通道，以便过滤除了通道和引入端口之间的连接处可能阻挡通道的颗粒或其它污染物 。
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公开(公告)号：US6149787A
公开(公告)日：2000-11-21
申请号：US173469
申请日：1998-10-14
Applicant: Andrea W. Chow , Robert S. Dubrow , J. Wallace Parce , Steven A. Sundberg , Jeffrey A. Wolk
Inventor： Andrea W. Chow , Robert S. Dubrow , J. Wallace Parce , Steven A. Sundberg , Jeffrey A. Wolk
CPC classification number: B01L3/502715 , B01L3/502746 , B01L2200/027 , B01L2300/0816 , B01L2300/0838 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , B01L3/50273 , G01N2035/00237 , G01N2035/1062 , Y10S366/02 , Y10T436/2575
Abstract: Methods, apparatus and systems are provided for introducing large numbers of different materials into a microfluidic analytical device rapidly, efficiently and reproducibly. In particular, improved integrated pipettor chip configurations, e.g. sippers or electropipettors, are described which are capable of sampling extremely small amounts of material for which analysis is desired, transporting material into a microfluidic analytical channel network, and performing the desired analysis on the material.
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公开(公告)号：US5451683A
公开(公告)日：1995-09-19
申请号：US53124
申请日：1993-04-23
Applicant: Ronald W. Barrett , Michael C. Pirrung , Lubert Stryer , Christopher P. Holmes , Steven A. Sundberg
Inventor： Ronald W. Barrett , Michael C. Pirrung , Lubert Stryer , Christopher P. Holmes , Steven A. Sundberg
IPC: C07D233/38 , C07D491/048 , C07D495/04 , C40B40/06 , G01N33/543 , G01N33/552 , A61K31/415
CPC classification number: C07D495/04 , C07D233/38 , G01N33/54306 , G01N33/54353 , G01N33/552 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , Y02P20/55
Abstract: Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.
Abstract translation: 描述了用于在固体底物的表面上固定抗配体如抗体或抗原，激素或激素受体，寡核苷酸和多糖的方法和组合物用于各种用途。 这些方法提供了覆盖有笼式结合构件的表面，其包括在施加合适的能量源时能够被去除的保护基团。 表面上预定区域的空间照射辐射允许在表面上的活化区域上固定抗配体。 表面不同区域的照射周期和不同抗配体的固定允许在表面上限定的位点形成固定的抗配体基团。 抗配体的固定化基质允许对基质的某些抗配体具有高亲和力的配体的液体样品的同时筛选。 本发明的优选实施方案涉及将可光活化的生物素衍生物连接到表面。 生物素衍生物的光解活化形成对抗生物素蛋白具有强结合亲和力的生物素类似物。 生物素化的抗配体可以固定在先前用抗生物素蛋白处理的表面的活化区上。
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35.发明授权Method and apparatus for generating thermal melting curves in a microfluidic device 有权用于在微流体装置中产生热解曲线的方法和装置公开(公告)号：US08900811B2
公开(公告)日：2014-12-02
申请号：US11352452
申请日：2006-02-09
Applicant: Steven A. Sundberg , Michael R. Knapp , Ivor T. Knight , Deborah J. Boles , Aaron J. Rulison , Wesley B. Dong , Andrew G. Fabans , Allen R. Boronkay , Edward P. Donlon , Robert J. Moti , Michael Slater
Inventor： Steven A. Sundberg , Michael R. Knapp , Ivor T. Knight , Deborah J. Boles , Aaron J. Rulison , Wesley B. Dong , Andrew G. Fabans , Allen R. Boronkay , Edward P. Donlon , Robert J. Moti , Michael Slater
CPC classification number: G01N25/04 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L7/525 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/14 , B01L2300/18 , B01L2300/1833 , B01L2400/0409 , B01L2400/0415 , B01L2400/0487 , C12Q1/68 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/33 , G01N21/6428 , Y10T436/143333 , C12Q2565/629 , C12Q2565/101 , C12Q2527/107 , C12Q2527/101
Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
Abstract translation: 本发明提供了采用微流体技术产生分子熔融曲线的新颖方法和装置。 特别地，根据本发明的装置和方法可用于提供PCR扩增产物的分析。
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Patent Agency Ranking
36.发明授权公开(公告)号：US08070928B2
公开(公告)日：2011-12-06
申请号：US11967657
申请日：2007-12-31
Applicant: Steven A. Sundberg , Xing Su , Grace Credo
Inventor： Steven A. Sundberg , Xing Su , Grace Credo
CPC classification number: C12N15/1006
Abstract: Embodiments of the invention provide devices and methods for extracting nucleic acid molecules from solution using electric fields. The structures and methods of embodiments of the invention are suited to incorporation into micro and nano fluidic devices, such as lab-on-a-chip devices and micro total analysis systems.
Abstract translation: 本发明的实施方案提供了使用电场从溶液中提取核酸分子的装置和方法。 本发明的实施例的结构和方法适合于并入微纳米流体装置，例如片上实验室装置和微总体分析系统。
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37.发明申请公开(公告)号：US20090166205A1
公开(公告)日：2009-07-02
申请号：US11967657
申请日：2007-12-31
Applicant: Steven A. Sundberg , Xing Su , Grace Credo
Inventor： Steven A. Sundberg , Xing Su , Grace Credo
CPC classification number: C12N15/1006
Abstract: Embodiments of the invention provide devices and methods for extracting nucleic acid molecules from solution using electric fields. The structures and methods of embodiments of the invention are suited to incorporation into micro and nano fluidic devices, such as lab-on-a-chip devices and micro total analysis systems.
Abstract translation: 本发明的实施方案提供了使用电场从溶液中提取核酸分子的装置和方法。 本发明的实施例的结构和方法适合于并入微纳米流体装置，例如片上实验室装置和微总体分析系统。
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38.发明授权Microfabricated structures for facilitating fluid introduction into microfluidic devices 有权用于促进流体引入微流体装置的微型结构公开(公告)号：US07364703B2
公开(公告)日：2008-04-29
申请号：US11493996
申请日：2006-07-27
Applicant: Steven A. Sundberg , J. Wallace Parce , Calvin Y. H. Chow
Inventor： Steven A. Sundberg , J. Wallace Parce , Calvin Y. H. Chow
CPC classification number: G01N27/44791 , B01J19/0093 , B01J2219/00783 , B01J2219/00826 , B01J2219/00831 , B01J2219/00833 , B01J2219/00853 , B01J2219/00891 , B01J2219/00909 , B01L3/0244 , B01L3/0262 , B01L3/5025 , B01L3/50255 , B01L3/5027 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502753 , B01L2200/027 , B01L2300/0681 , B01L2300/0816 , B01L2300/0819 , B01L2300/0864 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0688 , G01N27/44743 , G01N2035/00237 , G01N2035/1037 , Y10T436/25125 , Y10T436/25375 , Y10T436/2575
Abstract: Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.
Abstract translation: 通过使用完全延伸通过微流体衬底的端口来促进流体引入。 可以使用毛细管力将流体保持在端口内，并且可以通过单个端口引入一系列样品或其它流体，通过依次将流体通过基底吹出，并用替代流体代替去除的流体，或者通过置换 流体部分附加流体。 在另一方面，微流体衬底具有在横截面尺寸上变化的通道，使得毛细管作用仅在通道网络的有限部分内扩散流体。 在另一方面，引入端口可以包括从端口引导到流体通道的多个非常小的通道，以便过滤除了通道和引入端口之间的连接处可能阻挡通道的颗粒或其它污染物 。
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39.发明授权Apparatus and methods for correcting for variable velocity in microfluidic systems 有权用于校正微流体系统中可变速度的装置和方法公开(公告)号：US06703205B2
公开(公告)日：2004-03-09
申请号：US10102149
申请日：2002-03-19
Applicant: Anne R. Kopf-Sill , Andrea W. Chow , Claudia B. Cohen , Steven A. Sundberg , John Wallace Parce
Inventor： Anne R. Kopf-Sill , Andrea W. Chow , Claudia B. Cohen , Steven A. Sundberg , John Wallace Parce
IPC: G01N33558
CPC classification number: G01N33/557 , B01J19/0093 , B01J2219/0095 , B01J2219/0097 , B01J2219/00995 , B01L3/5027 , B01L3/50273 , B01L3/502746 , B01L3/563 , B01L9/527 , B01L2200/14 , B01L2200/143 , B01L2300/0627 , B01L2300/0816 , B01L2300/0867 , B01L2400/0415 , G01N27/447 , G01N27/44726 , G01N27/44791 , Y10S435/81 , Y10S436/805 , Y10S436/809
Abstract: Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
Abstract translation: 提供了具有用于校正电动效果的计算机的电动装置。 还提供了通过在电动微流体装置中确定反应物和产物在反应中的速度来校正电动效应的方法。 这些微流体装置可以具有用于移动，混合和监测精确量的分析物流体的通道，凹陷和/或孔的基底。
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公开(公告)号：US5482867A
公开(公告)日：1996-01-09
申请号：US54121
申请日：1993-04-23
Applicant: Ronald W. Barrett , Michael C. Pirrung , Lubert Stryer , Christopher P. Holmes , Steven A. Sundberg
Inventor： Ronald W. Barrett , Michael C. Pirrung , Lubert Stryer , Christopher P. Holmes , Steven A. Sundberg
IPC: C07D233/38 , C07D491/048 , C07D495/04 , C40B40/06 , G01N33/543 , G01N33/552
CPC classification number: C07D495/04 , C07D233/38 , G01N33/54306 , G01N33/54353 , G01N33/552 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00659 , B01J2219/00722 , C40B40/06 , Y02P20/55
Abstract: Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.
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