公开(公告)号：US07820427B2

公开(公告)日：2010-10-26

申请号：US11531147

申请日：2006-09-12

Applicant: Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Wienecke ,  Geoffrey Facer

IPC: C12M1/38

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：US20100184202A1

公开(公告)日：2010-07-22

申请号：US12579347

申请日：2009-10-14

Applicant: Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

Inventor： Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

IPC: C12M1/34

CPC classification number: C12Q1/68 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/686 ,  G01N27/453

Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US07704735B2

公开(公告)日：2010-04-27

申请号：US11740735

申请日：2007-04-26

Applicant: Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Philip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

Inventor： Geoffrey Facer ,  Robert Grossman ,  Marc Unger ,  Philip Lam ,  Hou-Pu Chou ,  Jake Kimball ,  Martin Pieprzyk ,  Antoine Daridon

IPC: C12M1/00 ,  C12M3/00

CPC classification number: C12M1/00 ,  B01J2219/00317 ,  B01J2219/00495 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T29/49826 ,  Y10T436/25

Abstract: Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.

Abstract translation: 提供方法和系统用于在选定的温度或时间范围内进行反应。 提供阵列装置。 阵列装置包含单独的反应室，并且由多层形成为弹性体块。 至少一个层具有至少一个凹部，该凹部具有至少一个与该凹部一体的可偏转膜。 阵列装置具有靠近至少一个反应室的热转印装置。 热转印装置形成为接触热控制源。 用于进行所需反应的试剂被引入阵列装置。 阵列装置与热控制装置接触，使得热控制装置与热控制源热连通，使得至少一个反应室中的反应温度由于温度变化而改变 的热控制源。

公开(公告)号：US07691333B2

公开(公告)日：2010-04-06

申请号：US10819088

申请日：2004-04-05

Applicant: Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer ,  Yong Yi

Inventor： Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer ,  Yong Yi

IPC: B01L3/00

CPC classification number: B01L3/00 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/6844 ,  C40B60/08 ,  C40B60/14 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T436/25 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US07604965B2

公开(公告)日：2009-10-20

申请号：US11084357

申请日：2005-03-18

Applicant: Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

Inventor： Lincoln McBride ,  Geoffrey Facer ,  Marc Unger ,  Michael Lucero ,  Hany Ramez Nassef

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/68 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/686 ,  G01N27/453

Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的MxN矩阵微流体装置，所述装置具有多个反应池，其通过形成在所述装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US20090176230A1

公开(公告)日：2009-07-09

申请号：US12291319

申请日：2008-11-06

Applicant: Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

Inventor： Lincoln McBride ,  Michael Lucero ,  Marc Unger ,  Hany Ramez Nassef ,  Geoffrey Facer

IPC: C12Q1/68 ,  B01J19/00 ,  G01N33/00

CPC classification number: B01L3/00 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/6844 ,  C40B60/08 ,  C40B60/14 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T436/25 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：US20090061428A1

公开(公告)日：2009-03-05

申请号：US11929436

申请日：2007-10-30

Applicant: Lincoln McBride ,  Geoffrey Facer ,  Marc Unger

Inventor： Lincoln McBride ,  Geoffrey Facer ,  Marc Unger

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L3/0248 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/686 ,  G01N27/453

Abstract: An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

Abstract translation: 一种用于执行反应矩阵的M次.N基质微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。

公开(公告)号：US20080088952A1

公开(公告)日：2008-04-17

申请号：US11953538

申请日：2007-12-10

Applicant: Marc Unger ,  Geoffrey Facer ,  Barry Clerkson ,  Christopher Cesar ,  Neil Switz

Inventor： Marc Unger ,  Geoffrey Facer ,  Barry Clerkson ,  Christopher Cesar ,  Neil Switz

IPC: G02B27/02 ,  G01N21/76 ,  G02B7/02

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

公开(公告)号：US07216671B2

公开(公告)日：2007-05-15

申请号：US11056451

申请日：2005-02-10

Applicant: Marc Unger ,  Hou-Pu Chou ,  Todd Thorsen ,  Axel Scherer ,  Stephen Quake ,  Markus Enzelberger ,  Mark Adams ,  Carl Hansen

Inventor： Marc Unger ,  Hou-Pu Chou ,  Todd Thorsen ,  Axel Scherer ,  Stephen Quake ,  Markus Enzelberger ,  Mark Adams ,  Carl Hansen

IPC: F15C1/06

CPC classification number: B05D3/04 ,  B01J2219/00355 ,  B01J2219/00378 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00439 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/06 ,  B01L2400/0655 ,  B01L2400/0688 ,  B32B2037/1081 ,  B65B31/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  C12Q1/6832 ,  C12Q1/6874 ,  C30B29/54 ,  F04B43/043 ,  F15C1/06 ,  F15C3/00 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0324 ,  Y10T137/0379 ,  Y10T137/0424 ,  Y10T137/206 ,  Y10T137/2082 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2174 ,  Y10T137/2202 ,  Y10T137/2224 ,  Y10T137/3084 ,  Y10T137/7837 ,  Y10T137/86027 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24612 ,  C12Q2535/125

Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

公开(公告)号：US07118910B2

公开(公告)日：2006-10-10

申请号：US10306798

申请日：2002-11-27

Applicant: Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Weinecke ,  Geoffrey Facer

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Weinecke ,  Geoffrey Facer

IPC: F16K11/20

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.