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公开(公告)号：US20050277125A1
公开(公告)日：2005-12-15
申请号：US10976168
申请日：2004-10-27
申请人： James Benn , Mats Cooper , Todd Thorsen
发明人： James Benn , Mats Cooper , Todd Thorsen
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00369 , B01J2219/00527 , B01J2219/00563 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00657 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , B01L3/021 , B01L3/0293 , B01L3/5025 , B01L3/502715 , B01L3/563 , B01L9/543 , B01L2200/021 , B01L2200/025 , B01L2200/027 , B01L2300/0819 , B01L2300/0829 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0487 , G01N35/1011 , G01N2035/00158 , G01N2035/1034
摘要： Methods and devices for performing multiple simultaneous reactions on a reaction surface are disclosed. Methods and devices for simultaneously interrogating multiple patient samples with multiple diagnostic reagents are disclosed.
摘要翻译： 公开了在反应表面上进行多次同时反应的方法和装置。 公开了用多个诊断试剂同时询问多个患者样品的方法和装置。
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公开(公告)号：US08592215B2
公开(公告)日：2013-11-26
申请号：US13249212
申请日：2011-09-29
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
IPC分类号： G01N35/08 , G01N33/483
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： This invention provides microfabricated devices and methods for detecting, analyzing and sorting biological materials and particles. Droplets containing the particles are provided in an extrusion fluid, passed through a detection region, and then directed into a branch channel according to predetermined characteristics. For example, cells or viral particles contained in droplets of aqueous solvent are flowed past a detector in the nonpolar extrusion fluid decane, and routed into a selected branch channel for subsequent analysis or use.
摘要翻译： 本发明提供用于检测，分析和分选生物材料和颗粒的微制造装置和方法。 将含有颗粒的液滴设置在挤出流体中，通过检测区域，然后根据预定特性被引导到分支通道中。 例如，包含在水性溶剂的液滴中的细胞或病毒颗粒流过非极性挤压流体癸烷中的检测器，并且路由到选择的分支通道用于随后的分析或使用。
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公开(公告)号：US20120276543A1
公开(公告)日：2012-11-01
申请号：US13475868
申请日：2012-05-18
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
摘要翻译： 提供微流体装置用于分析或分选生物材料，例如多核苷酸，多肽，蛋白质，酶，病毒和细胞。 本发明可用于高通量或组合筛选。 该装置包括在液滴挤出区域连通的主通道和入口通道，使得溶液液滴沉积在主通道中的不混溶溶剂中。 此后可以根据在每个液滴中检测到的生物材料对水滴进行分类。
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公开(公告)号：US07294503B2
公开(公告)日：2007-11-13
申请号：US09953103
申请日：2001-09-14
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.
摘要翻译： 提供了用于分析和/或分选生物材料的微流体装置（例如分子，例如多核苷酸和多肽，包括蛋白质和酶;病毒和细胞）及其使用方法。 本发明的装置和方法可用于分选颗粒，例如 病毒粒子。 本发明也可用于高通量筛选，例如 组合筛选 微流体装置包括在液滴挤出区域处与主通道连通的主通道和入口区域。 含有生物材料的溶液的液滴通过液滴挤出区沉积到主通道中。 与含有生物材料的溶液不同且不相容的流体流过主通道，使得含有生物材料的液滴不会扩散或混合。 可以通过检测每个液滴中的生物样品的预定特征并相应地分选液滴来分析和/或分选液滴内的生物材料。
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公开(公告)号：US07216671B2
公开(公告)日：2007-05-15
申请号：US11056451
申请日：2005-02-10
申请人： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake , Markus Enzelberger , Mark Adams , Carl Hansen
发明人： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake , Markus Enzelberger , Mark Adams , Carl Hansen
IPC分类号： F15C1/06
CPC分类号： B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译： 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。
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公开(公告)号：US20120231444A1
公开(公告)日：2012-09-13
申请号：US13249212
申请日：2011-09-29
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： This invention provides microfabricated devices and methods for detecting, analyzing and sorting biological materials and particles. Droplets containing the particles are provided in an extrusion fluid, passed through a detection region, and then directed into a branch channel according to predetermined characteristics. For example, cells or viral particles contained in droplets of aqueous solvent are flowed past a detector in the nonpolar extrusion fluid decane, and routed into a selected branch channel for subsequent analysis or use.
摘要翻译： 本发明提供用于检测，分析和分选生物材料和颗粒的微制造装置和方法。 将含有颗粒的液滴设置在挤出流体中，通过检测区域，然后根据预定特性被引导到分支通道中。 例如，包含在水性溶剂的液滴中的细胞或病毒颗粒流过非极性挤压流体癸烷中的检测器，并且路由到选择的分支通道用于随后的分析或使用。
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公开(公告)号：US20090035838A1
公开(公告)日：2009-02-05
申请号：US11868942
申请日：2007-10-08
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.
摘要翻译： 提供了用于分析和/或分选生物材料的微流体装置（例如分子，例如多核苷酸和多肽，包括蛋白质和酶;病毒和细胞）及其使用方法。 本发明的装置和方法可用于分选颗粒，例如 病毒粒子。 本发明也可用于高通量筛选，例如 组合筛选 微流体装置包括在液滴挤出区域处与主通道连通的主通道和入口区域。 含有生物材料的溶液的液滴通过液滴挤出区沉积到主通道中。 与含有生物材料的溶液不同且不相容的流体流过主通道，使得含有生物材料的液滴不会扩散或混合。 可以通过检测每个液滴中的生物样品的预定特征并相应地分选液滴来分析和/或分选液滴内的生物材料。
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公开(公告)号：US20050112882A1
公开(公告)日：2005-05-26
申请号：US10945737
申请日：2004-09-20
申请人： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake
发明人： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake
IPC分类号： G03F7/20 , B01L3/00 , B81B1/00 , B81B3/00 , B81B7/00 , B81C1/00 , B81C99/00 , C12Q1/68 , F04B17/00 , F04B35/04 , F04B43/04 , F15C5/00 , F16K7/00 , F16K17/02 , F16K99/00 , H01L21/302 , H01L21/461
CPC分类号： B29C51/00 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/046 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B81B5/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , B81C2201/034 , C12Q1/6874 , F04B43/043 , F15C1/06 , F15C5/00 , F16K11/20 , F16K13/00 , F16K31/126 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0048 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0094 , Y10T137/0491 , Y10T137/0497 , Y10T137/2224 , Y10T137/87249 , Y10T156/10 , Y10T428/24479 , Y10T428/24744 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
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公开(公告)号：US08658368B2
公开(公告)日：2014-02-25
申请号：US13475879
申请日：2012-05-18
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
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公开(公告)号：US20120276544A1
公开(公告)日：2012-11-01
申请号：US13475879
申请日：2012-05-18
申请人： Stephen R. Quake , Todd Thorsen
发明人： Stephen R. Quake , Todd Thorsen
CPC分类号： C12Q1/68 , B01L3/502707 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/12 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/12 , B01L2300/123 , B01L2400/0418 , B01L2400/0421 , B01L2400/0454 , B01L2400/0481 , B01L2400/0484 , B01L2400/0487 , B01L2400/0638 , C12M1/34 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N33/54366 , G01N35/08 , G01N2015/0065 , G01N2015/0088 , G01N2015/1006 , G01N2015/1081 , G01N2015/1411 , G01N2015/1481 , G01N2015/149 , Y10T436/118339 , Y10T436/143333
摘要： A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
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