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48 条记录 (耗时 0.034 秒)

    Fluorescent stokes shift probes for polynucleotide hybridization
    43.
    发明授权
    Fluorescent stokes shift probes for polynucleotide hybridization 失效
    荧光斯托克斯移位探针用于多核苷酸杂交

    公开(公告)号：US4996143A

    公开(公告)日：1991-02-26

    申请号：US511834

    申请日：1990-04-13

    申请人： Michael J. Heller ,  Edward J. Jablonski

    发明人： Michael J. Heller ,  Edward J. Jablonski

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/533

    CPC分类号： G01N33/533 ,  C12Q1/6818 ,  C12Q1/705 ,  Y10S436/80

    摘要： Fluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.

    摘要翻译： 设计用于多核苷酸杂交测定的荧光斯托克斯移位探针以在供体和受体荧光团之间提供预定的核苷酸碱基单位间隔。 当探针与目标多核苷酸杂交时，配对用于非辐射能量转移的荧光团被分离2至7个核苷酸碱基单位。 荧光团通过长度为4至30埃的连接臂连接到DNA或RNA探针。 荧光素是与德克萨斯红受体一起使用的优选供体。

    Assay procedures
    44.
    发明授权
    Assay procedures 失效
    测定程序

    公开(公告)号：US4804625A

    公开(公告)日：1989-02-14

    申请号：US656011

    申请日：1984-09-27

    申请人： Larry E. Morrison ,  Garfield P. Royer ,  Michael J. Heller

    发明人： Larry E. Morrison ,  Garfield P. Royer ,  Michael J. Heller

    IPC分类号： G01N33/536 ,  C12Q1/28 ,  C12Q1/37 ,  C12Q1/42 ,  G01N33/535 ,  G01N33/543 ,  G01N33/563

    CPC分类号： G01N33/535 ,  Y10S435/81 ,  Y10S436/805

    摘要： Binding assay methods involving determining the presence of analytes in samples through enzymatic formation of detectable substances in amounts related to the amount of analyte present in the sample and monitoring for the presence of the substances in distinct phases. Methods according to the invention include use of labelled materials which associate with the analyte to be determined or compete with the analyte for association with an added binder. The labelled materials employed include label portions which enzymatically form substances from substrates provided in or existing as a first phase, or, upon enzymatic treatment in a first phase, disassociate into substances capable of existing in or as a second distinct phase. Formation of the detectable substances is monitored by determining the transfer of the substance to a second distinct phase in contact with the first phase or by determining formation of a second distinct phase. The assays are useful in determining human IgG protein in blood samples and other constituents of blood or other biological samples without elaborate instrumentation, allowing for practice outside the clinical laboratory.

    摘要翻译： 结合测定方法包括通过以与样品中存在的分析物的量相关的量的酶形成可检测物质来测定样品中分析物的存在并监测不同阶段中物质的存在。 根据本发明的方法包括使用与待测定的分析物相关联的标记材料或与分析物竞争与所添加的粘合剂缔合。 所使用的标记材料包括标记部分，其从在第一阶段中提供或存在的底物酶促形成物质，或者在第一阶段的酶处理中，分解成能够存在于或作为第二不同相的物质。 通过确定物质与第一相接触的第二不同相的转移或通过确定第二不同相的形成来监测可检测物质的形成。 该测定法可用于测定血液样本中血液或其他生物样品中其他成分的人类IgG蛋白质，而无需精心设计，可在临床实验室外进行实践。

    Apparatus for active biological sample preparation
    46.
    发明授权
    Apparatus for active biological sample preparation 失效

    公开(公告)号：US06824740B1

    公开(公告)日：2004-11-30

    申请号：US09561318

    申请日：2000-04-28

    申请人： Edward L. Sheldon, III ,  Thomas R. Jackson ,  Paul D. Swanson ,  Bradley S. Scott ,  Michael J. Heller

    发明人： Edward L. Sheldon, III ,  Thomas R. Jackson ,  Paul D. Swanson ,  Bradley S. Scott ,  Michael J. Heller

    IPC分类号： B01L1100

    CPC分类号： B01D57/02 ,  B01J19/0093 ,  B01L3/502753 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  C12N13/00 ,  G01N27/44743 ,  H01L29/6656

    摘要： Systems and methods for the electronic sample preparation of biological materials utilize the differential charge-to-mass ratio and/or the differential affinity of sample constituents to separation materials for sample preparation. An integrated system is provided for performing some or all of the processes of: receipt of biological materials, cell selection, sample purification, sample concentration, buffer exchange, complexity reduction and/or diagnosis and analysis. In one embodiment, one or more sample chambers adapted to receive a buffer solution are formed adjacent to a spacer region which may include a trap or other affinity material, electrophoretic motion of the materials to be prepared being effected through operation of electrodes. In another aspect of this invention, a transporter or dipstick serves to collect and permit transport of materials, such as nucleic acids, most preferably DNA and/or RNA. In one embodiment, a membrane or trap is held in a frame which is adapted to mate with a channel formed in the spacer region. In another aspect of this invention, an electrophoretic system for biological sample preparation is operated in a manner so as to utilize the differential charge-to-mass ratio so as to control the migration of materials within the solution. In one aspect, bunching of selected materials is achieved by operation of two electrodes in a manner so as to reduce the spatial dispersion of those materials. In another aspect of this invention, a vertically disposed sample preparation unit includes an upper reservoir including and a collection chamber. A sample is preferably pre-prepared and densified, applied to the conductive polymer, electrophoresed so as to move nucleic acids into the conductive polymer and move undesired material away from the conductive polymer. Integrated systems are described in which cell separation, purification, complexity reduction and diagnosis may be performed together. In the preferred embodiment, cell separation and sample purification are performed in a first region, the steps of denaturation, complexity reduction and diagnosis being performed in a second region.