中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

67 records (took 0.033 seconds)

    Spatially-addressable immobilization of anti-ligands on surfaces
    65.
    发明授权
    Spatially-addressable immobilization of anti-ligands on surfaces 失效
    表面上抗配体的空间可寻址固定

    公开(公告)号：US5252743A

    公开(公告)日：1993-10-12

    申请号：US612671

    申请日：1990-11-13

    Applicant: Ronald W. Barrett ,  Michael C. Pirrung ,  Lubert Stryer ,  Christopher P. Holmes ,  Steven A. Sundberg

    Inventor： Ronald W. Barrett ,  Michael C. Pirrung ,  Lubert Stryer ,  Christopher P. Holmes ,  Steven A. Sundberg

    IPC: C07D233/38 ,  C07D491/048 ,  C07D495/04 ,  C40B40/06 ,  G01N33/543 ,  G01N33/552 ,  C07D235/02

    CPC classification number: C07D495/04 ,  C07D233/38 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/552 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  Y02P20/55

    Abstract: Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.

    Abstract translation: 描述了用于在固体底物的表面上固定抗配体如抗体或抗原，激素或激素受体，寡核苷酸和多糖的方法和组合物用于各种用途。 这些方法提供了覆盖有笼式结合构件的表面，其包括在施加合适的能量源时能够被去除的保护基团。 表面上预定区域的空间照射辐射允许在表面上的活化区域上固定抗配体。 表面不同区域的照射周期和不同抗配体的固定允许在表面上限定的位点形成固定的抗配体基团。 抗配体的固定化基质允许对基质的某些抗配体具有高亲和力的配体的液体样品的同时筛选。 本发明的优选实施方案涉及将可光活化的生物素衍生物连接到表面。 生物素衍生物的光解活化形成对抗生物素蛋白具有强结合亲和力的生物素类似物。 生物素化的抗配体可以固定在先前用抗生物素蛋白处理的表面的活化区上。

    Fluorescent conjugates for analysis of molecules and cells
    66.
    发明授权
    Fluorescent conjugates for analysis of molecules and cells 失效
    用于分析和细胞分析的荧光偶联物

    公开(公告)号：US5055556A

    公开(公告)日：1991-10-08

    申请号：US791248

    申请日：1985-10-25

    Applicant: Lubert Stryer ,  Alexander N. Glazer ,  Vernon T. Oi

    Inventor： Lubert Stryer ,  Alexander N. Glazer ,  Vernon T. Oi

    IPC: G01N33/533

    CPC classification number: G01N33/533 ,  Y10S436/80 ,  Y10S436/805

    Abstract: Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.

    Abstract translation: 通过使用具有胆汁假体基团的荧光蛋白作为标记来提供这种技术的敏感检测技术和组合物。 含有蛋白质的蛋白质可以与配体或受体结合，用于涉及配体 - 受体结合的系统，用于配体和受体的分析，检测或分离。 特别地，一种或多种含胆红蛋白的蛋白质可以用作彼此结合的标记或用于定义天然存在的聚集子集的其它荧光剂。 细胞。

    Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor
    67.
    发明授权
    Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor 失效
    使用藻胆蛋白标记的配体或受体进行荧光免疫测定

    公开(公告)号：US4520110A

    公开(公告)日：1985-05-28

    申请号：US454768

    申请日：1982-12-30

    Applicant: Lubert Stryer ,  Alexander N. Glazer ,  Vernon T. Oi

    Inventor： Lubert Stryer ,  Alexander N. Glazer ,  Vernon T. Oi

    IPC: G01N33/533 ,  G01N33/54 ,  G01N31/00

    CPC classification number: G01N33/533 ,  Y10S436/80 ,  Y10S530/825

    Abstract: Sensitive detection techniques and compositions for such techniques employing fluorescent proteins having bilin prosthetic groups as labels i.e. phycobiliprotein. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjuction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.

    Abstract translation: 敏感检测技术和使用具有胆汁假体基团的荧光蛋白质的技术的组合物，即藻胆蛋白。 含有蛋白质的蛋白质可以与配体或受体结合，用于涉及配体 - 受体结合的系统，用于配体和受体的分析，检测或分离。 特别地，一种或多种含胆红蛋白的蛋白质可以用作彼此结合的标记或用于定义天然存在的聚集子集的其它荧光剂。 细胞。

Patent Agency Ranking