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    Methods and systems for molecular fingerprinting
    63.
    发明申请
    Methods and systems for molecular fingerprinting 审中-公开
    分子指纹图谱的方法和系统

    公开(公告)号：US20070141599A1

    公开(公告)日：2007-06-21

    申请号：US11582838

    申请日：2006-10-17

    Applicant: Stephen Quake ,  Hou-Pu Chou

    Inventor： Stephen Quake ,  Hou-Pu Chou

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0484 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0633 ,  C12Q1/6816 ,  C12Q1/683 ,  G01N2015/149 ,  C12Q2565/629

    Abstract: This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacterial typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.

    Abstract translation: 本发明一般涉及分子指纹图谱的方法。 该方法可用于法医鉴定（例如DNA指纹图谱，特别是VNTR），细菌分型和人/动物病原体诊断。 更具体地，可以按照限制性片段长度多态性分析多核苷酸的微制造装置中的大小来评估或分类分子，例如多核苷酸（例如DNA）。 在根据本发明的微制造装置中，通过测量例如与多核苷酸片段相关的可光学检测（例如，荧光）报告基因的信号，可以使用相对小的样品快速和精确地分型DNA片段或其他分子。

    Combinational array for nucleic acid analysis
    68.
    发明申请
    Combinational array for nucleic acid analysis 审中-公开
    用于核酸分析的组合阵列

    公开(公告)号：US20050196785A1

    公开(公告)日：2005-09-08

    申请号：US11030620

    申请日：2005-01-05

    Applicant: Stephen Quake ,  Robert Van Dam

    Inventor： Stephen Quake ,  Robert Van Dam

    IPC: C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

    CPC classification number: C12Q1/6832 ,  G16B25/00 ,  G16B40/00 ,  C12Q2525/204 ,  C12Q2565/514

    Abstract: This invention relates to an array, including a universal micro-array, for the analysis of nucleic acids, such as DNA. The devices and methods of the invention can be used for identifying gene expression patterns in any organism. More specifically, all possible oligonucleotides (n-mers) necessary for the identification of gene expression patterns are synthesized. According to the invention, n is large enough to give the specificity to uniquely identify the expression pattern of each gene in an organism of interest, and is small enough that the method and device can be easily and efficiently practiced and made. The invention provides a method of analyzing molecules, such as polynucleotides (e.g., DNA), by measuring the signal of an optically-detectable (e.g., fluorescent, ultraviolet, radioactive or color change) reporter associated with the molecules. In a polynucleotide analysis device according to the invention, levels of gene expression are correlated to a signal from an optically-detectable (e.g. fluorescent) reporter associated with a hybridized polynucleotide. The invention includes an algorithm and method to interpret data derived from a micro-array or other device, including techniques to decode or deconvolve potentially ambiguous signals into unambiguous or reliable gene expression data.

    Abstract translation: 本发明涉及用于分析核酸如DNA的包括通用微阵列的阵列。 本发明的装置和方法可用于鉴定任何生物体中的基因表达模式。 更具体地，合成鉴定基因表达模式所需的所有可能的寡核苷酸（n-mers）。 根据本发明，n足够大以给出唯一地识别感兴趣的生物体中每个基因的表达模式的特异性，并且足够小以使得该方法和装置可以容易且有效地实施和制造。 本发明提供了通过测量与分子相关的光学可检测（例如荧光，紫外线，放射性或颜色变化）报告基因的信号来分析分子的方法，例如多核苷酸（例如DNA）。 在根据本发明的多核苷酸分析装置中，基因表达水平与来自与杂交多核苷酸相关的光学可检测（例如荧光）报告基因的信号相关。 本发明包括用于解释从微阵列或其他设备导出的数据的算法和方法，包括将潜在的模糊信号解码或解卷解为明确或可靠的基因表达数据的技术。

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