公开(公告)号：US20180346881A1

公开(公告)日：2018-12-06

申请号：US15562288

申请日：2016-03-31

申请人： BOEHRINGER INGELHEIM INTERNATIONAL GMBH

发明人： Christoph CLEMENS ,  Jochen SCHAUB ,  Marie LINK ,  Peter SCHORN ,  Torsten SCHULZ

IPC分类号： C12N5/071 ,  C07K16/28

CPC分类号： C12N5/0031 ,  C12N2500/02 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2511/00

摘要： The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.

公开(公告)号：US10017740B2

公开(公告)日：2018-07-10

申请号：US14664441

申请日：2015-03-20

申请人： PLASTICELL LIMITED

发明人： Marina Tarunina ,  Yen Choo ,  Mylvaganam Jeyakumar ,  Thomas Watson ,  Meritxell Rosell ,  Lilian Hook ,  Diana Hernandez

IPC分类号： C12N5/00 ,  C12N5/077 ,  A61K35/35

CPC分类号： C12N5/0653 ,  A61K35/35 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2501/01 ,  C12N2501/04 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2502/1352 ,  C12N2506/1384

摘要： The present invention relates to brown adipose tissue (BAT) cells derived from adult stern or progenitor cells, derived from adult white fat tissue (WAT), as well as to methods for deriving such cells.

公开(公告)号：US20180051250A1

公开(公告)日：2018-02-22

申请号：US15718574

申请日：2017-09-28

申请人： AJINOMOTO CO., INC.

发明人： Takuya MATSUMOTO ,  Sho SENDA ,  Tsuyoshi KOBAYASHI ,  Akihiro ARAKAWA

IPC分类号： C12N5/0797

CPC分类号： C12N5/0623 ,  A61K35/30 ,  A61L27/00 ,  C12N5/10 ,  C12N2500/24 ,  C12N2500/46 ,  C12N2501/115 ,  C12N2506/03 ,  C12N2506/45

摘要： Culture media, which contain chelated iron, promote cell proliferation of neural stem cells and/or neural progenitor cells while maintaining undifferentiated state and multipotency.

公开(公告)号：US20170369836A1

公开(公告)日：2017-12-28

申请号：US15619515

申请日：2017-06-11

申请人： Zhaoqing Dahuanong Biological Medicine Co., Ltd ,  East China University of Science and Technology ,  Guangdong Wens Dahuanong Biotechnology Co., Ltd.

发明人： RUIAI CHEN ,  Hanzhang Lai ,  Wensong Tan ,  Xuanzi Zhan ,  Xuping Liu ,  Kangcong MAI ,  Weilan Pan ,  Xiaofen Wang ,  Huajian Chen ,  Peijun Chen ,  Jiahua Xu ,  Qin Tang ,  Wenyan Zhang ,  Yan Gao

IPC分类号： C12N5/00

CPC分类号： C12N5/00 ,  C12N5/0037 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/76 ,  C12N2500/90

摘要： The present invention discloses a serum-free medium for full suspension culture of MDCK cells and a preparation method of the serum-free medium. The serum-free medium for full suspension culture of the MDCK cells comprises basic metabolic nutrients, nucleotide, vitamins, inorganic salts, a shear force protective agent, a cell clustering resisting agent, a pH buffer agent, a pH indicator, an influenza virus proliferation accelerant and other additives. The preparation method of the serum-free medium for the full suspension culture of the MDCK cells comprises the following steps: 1) preparing a mixed solution: dissolving and mixing raw materials; and 2) regulating pH: regulating the pH of the mixed solution to 6.3 to 6.7, and setting a constant volume to obtain the serum-free medium for the full suspension culture of the MDCK cells. The medium supports the high-density full-suspension culture of the MDCK single cells, greatly shortens a time for educating the MDCK cells from adherent cells into the serum-free full suspension cells, and is applicable to the mass production of biological products, and particularly veterinary biological products.

公开(公告)号：US09650604B2

公开(公告)日：2017-05-16

申请号：US14359974

申请日：2012-11-22

申请人： Kang Stem Biotech, Co., LTD.

发明人： Kyung Sun Kang ,  Min Soo Seo ,  Sang Bum Park

IPC分类号： A61K35/50 ,  C12N5/074 ,  C12N5/0775

CPC分类号： C12N5/0607 ,  A61K35/50 ,  C12N5/0668 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/00 ,  C12N2501/33 ,  C12N2506/03

摘要： The present invention relates to equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs) and a preparation method thereof. More specifically, the present invention relates to equine amniotic membrane-derived mesenchymal stem cells, which show negative immunological responses to all of the human markers CD19, CD20, CD28, CD31, CD34, CD38, CD41a, CD62L, CD62P and CD200, and positive immunological responses to all of the human markers CD44, CD90 and CD105, and have the ability to be maintained in an undifferentiated state for 14 passages or more and the ability to differentiate into ectoderm, mesoderm and endoderm-derived cells.

公开(公告)号：US20170130191A1

公开(公告)日：2017-05-11

申请号：US15187425

申请日：2016-06-20

申请人： AbbVie Inc.

发明人： Itzcoatl A. Pla ,  Joseph G. Matuck ,  John C. Fann ,  Christof Schulz ,  Nichole A. Roy ,  David F. Bruton ,  James McIntire ,  Yu-hsiang D. Chang ,  Thomas Seewoester

IPC分类号： C12N5/00

CPC分类号： C12N5/0037 ,  C07K16/00 ,  C07K16/241 ,  C07K16/244 ,  C07K16/2869 ,  C07K2317/14 ,  C07K2317/21 ,  C12N5/0031 ,  C12N5/0603 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/60 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2501/10 ,  C12N2501/33 ,  C12N2510/02 ,  C12N2511/00

摘要： The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.

公开(公告)号：US20160281060A1

公开(公告)日：2016-09-29

申请号：US15166965

申请日：2016-05-27

申请人： StemRD, Inc.

发明人： Songzhu An ,  Yanan Zhu

IPC分类号： C12N5/0775 ,  C12N5/077 ,  A61K35/32 ,  C12N5/00 ,  A61K35/28

CPC分类号： C12N5/0662 ,  A61K35/28 ,  A61K35/32 ,  C12N5/0037 ,  C12N5/0655 ,  C12N5/0663 ,  C12N5/0665 ,  C12N5/0667 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/825 ,  C12N2506/1346

摘要： Embodiments of chemically defined cell culture media containing nutrients and growth factors free of any serum for culturing cells such as mesenchymal stem cells and methods of using embodiments of the cell culture medium for expanding cell populations such as mesenchymal stem cells while maintaining a pluripotent phenotype and methods of inducing chondrogenesis and osteogenesis of mesenchymal stem cells by admixing differentiation factors into embodiments of the cell culture medium.

公开(公告)号：US09434922B2

公开(公告)日：2016-09-06

申请号：US14639454

申请日：2015-03-05

申请人： Washington State University

发明人： Jon Michael Oatley ,  Melissa Joan Oatley

IPC分类号： A61B17/43 ,  C12N5/00 ,  C12N5/076 ,  C12N5/073 ,  C12N5/071 ,  C12M1/00 ,  A61K35/52 ,  C12N5/077 ,  C12N15/873 ,  A01K67/027 ,  A61B17/425 ,  A61D19/00

CPC分类号： C12N5/061 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/101 ,  A01K2227/108 ,  A01K2267/02 ,  A61B17/425 ,  A61D19/00 ,  A61K35/52 ,  C12M21/00 ,  C12N5/0602 ,  C12N5/0603 ,  C12N5/0656 ,  C12N5/0683 ,  C12N15/873 ,  C12N2500/05 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/21 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/246 ,  C12N2510/04 ,  C12N2533/90

摘要： The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

公开(公告)号：US09346869B2

公开(公告)日：2016-05-24

申请号：US14219157

申请日：2014-03-19

申请人： Yu-Hua Tseng ,  C. Ronald Kahn

发明人： Yu-Hua Tseng ,  C. Ronald Kahn

IPC分类号： A61K48/00 ,  C07K14/51 ,  C12N15/861 ,  C07K14/495 ,  C12N5/077 ,  C12N5/0775 ,  A61K35/12

CPC分类号： C07K14/495 ,  A61K35/12 ,  C12N5/0653 ,  C12N5/0667 ,  C12N2500/24 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/02 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2510/00

摘要： Methods and compositions for treating obesity and related disorders. The methods include the use of stem cells treated with BMP-2, -4, -5, -6 and/or -7.

摘要翻译： 用于治疗肥胖症和相关疾病的方法和组合物。 所述方法包括使用用BMP-2，-4，-5，-6和/或-7处理的干细胞。

公开(公告)号：US09295702B2

公开(公告)日：2016-03-29

申请号：US14580366

申请日：2014-12-23

申请人： TRANSGENE S.A.

发明人： Daniel Malarme ,  Yves Cordier ,  Claude Sene

IPC分类号： A61K35/76 ,  A61K39/12 ,  C07K14/82 ,  C12N7/00 ,  C12N15/86 ,  C12N7/02 ,  A61K48/00 ,  A61K39/00

CPC分类号： A61K35/76 ,  A61K39/0011 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/55533 ,  A61K2039/585 ,  C07K14/82 ,  C07K2319/01 ,  C12N7/00 ,  C12N7/02 ,  C12N15/86 ,  C12N2500/24 ,  C12N2501/11 ,  C12N2510/02 ,  C12N2710/20034 ,  C12N2710/24143 ,  C12N2710/24151

摘要： The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.